Voltage-gated calcium channels regulate K+ transport in the Malpighian tubules of the larval cabbage looper, Trichoplusia ni

Kolosov, Dennis; Leonard, Erin M.; O’Donnell, Michael J.

doi:10.1016/j.jinsphys.2021.104230

Cited by 6 publications

(8 citation statements)

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“…The expression of CaV1 genes has been detected in Drosophila Malpighian tubules, where their function is related to intracellular signalling, Ca 2+ transport and diuresis. 73 Kolosov et al 74 observed that CaV subunits are transcriptionally regulated in the Malpighian tubules of larval Trichoplusia ni depending on the potassium ion content in the diet, concluding that the voltage-gated calcium channels regulate K + transport in the gut. Given that cucumber plants are a rich source of potassium, an intriguing yet untested possibility is that P. persimilis struggles to maintain homeostasis while feeding on a high K + content diet, which necessitates differential expression of CaV genes.…”

Section: Discussionmentioning

confidence: 99%

Adaptation of an arthropod predator to a challenging environment is associated with a loss of a genome‐wide plastic transcriptional response

Bajda,

Wybouw,

Nguyễn

et al. 2024

Pest Management Science

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BackgroundStructural and chemical plant defense traits may reduce the efficacy of biological control agents in integrated pest management. Breeding programmes have shown arthropod predators' potential to acclimate to challenging host plants. However, whether and how these predators adapt to novel plant environments remains unclear. Using the predatory mite Phytoseiulus persimilis – herbivorous mite Tetranychus urticae system in an experimental evolution set‐up, we studied the adaptation mechanisms to tomato and cucumber, plants that possess a distinct repertoire of defensive traits.ResultsExperimental evolution experiments on whole plants revealed that allowing P. persimilis to adapt to tomatoes led to an approx. 100% larger population size. Independent feeding assays showed that tomato‐ and cucumber‐adapted prey reduced predator fecundity. The deleterious effect of ingesting low‐quality prey persisted after the adaptation of the predator to both cucumber and tomato. We demonstrated that JA‐dependent defences reduce prey quality by evaluating predator performance on prey fed on jasmonic acid (JA) defence‐deficient tomato plants. Transcriptomic profiling of the replicated P. persimilis lines showed that long‐term propagation on tomato and cucumber plants produces distinctive gene expression profiles. Predator adaptation to tomatoes results in the loss of a large transcriptional response, where predicted cuticle‐building rather than detoxification pathways are affected.ConclusionWe showed that the adaptation of predatory arthropods to novel, challenging plant does not necessarily occur via the prey but rather through the physical environment of the plant. We provided first insights into the underlying molecular mechanisms.This article is protected by copyright. All rights reserved.

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Section: Discussionmentioning

confidence: 99%

Adaptation of an arthropod predator to a challenging environment is associated with a loss of a genome‐wide plastic transcriptional response

Bajda,

Wybouw,

Nguyễn

et al. 2024

Pest Management Science

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“…4). A recent study has shown that Ca 2+ entry through voltage-gated calcium channels enables robust ion secretion by the distal ileac plexus (Kolosov et al, 2021) (Fig. 4A).…”

Section: Controlling the Direction Of Vectorial Ion Transport: Voltag...mentioning

confidence: 94%

“…In addition, transcript abundance of the voltagesensing subunit of a voltage-gated Ca 2+ channel is downregulated in the distal ileac plexus of larvae reared on a K + -rich diet, and this change, together with other transcriptional changes, enables the switch to ion reabsorption. In vitro, pharmacological inhibition of the voltage-gated Ca 2+ channel Ca V 1 reduces intracellular Ca 2+ concentration and reverses the direction of K + transport from secretion to reabsorption (Kolosov et al, 2021). Although Ca 2+ entry through voltage-gated channels is clearly required for ion secretion by the tubule, an important question remains: as the tubules are non-excitable, how do voltage-gated calcium channels receive the depolarizing stimulus necessary to open and support Ca 2+ influx in the absence of an action potential?…”

Section: Controlling the Direction Of Vectorial Ion Transport: Voltag...mentioning

confidence: 99%

“…For instance, pharmacological closing of gap junctions (Kolosov et al, 2018a) and inhibition of HCN channels (Kolosov and O'Donnell, 2019c) induce switching from ion secretion to ion reabsorption in the distal ileac plexus. In contrast, pharmacological inhibition of Ca V 1 (Kolosov et al, 2021), application of HK-1 and stimulation of ligand-gated ion channels O'Donnell, 2019c, 2020) regulate transport of specific ions without switching the distal ileac plexus between ion secretion and ion reabsorption. There are several potential reasons for this complexity.…”

Section: Controlling the Direction Of Vectorial Ion Transport: Voltag...mentioning

confidence: 99%

“…As discussed below, recent work has indicated a role for voltagedependent ion channels in regulating the switch (Kolosov et al, 2021;Kolosov and O'Donnell, 2019c).…”

mentioning

confidence: 99%

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Blending physiology and RNAseq to provide new insights into regulation of epithelial transport: switching between ion secretion and reabsorption

Kolosov

O’Donnell

2022

Journal of Experimental Biology

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This Review addresses the means by which epithelia change the direction of vectorial ion transport. Recent studies have revealed that insect Malpighian (renal) tubules can switch from secreting to reabsorbing K+. When the gut of larval lepidopterans is empty (during the moult cycle) or when the larvae are reared on K+-deficient diet, the distal ileac plexus segment of the tubule secretes K+ from the haemolymph into the tubule lumen. By contrast, in larvae reared on K+-rich diet, ions and fluid are reabsorbed from the rectal lumen into the perinephric space surrounding the cryptonephridial tubules of the rectal complex. Ions and fluid are then transported from the perinephric space into the lumen of the cryptonephridial tubules, thus supplying the free segments of the tubule downstream. Under these conditions, some of the K+ and water in the tubule lumen is reabsorbed across the cells of the distal ileac plexus, allowing for expansion of haemolymph volume in the rapidly growing larvae, as well as recycling of K+ and base equivalents. RNA sequencing data reveal large-scale changes in gene transcription that are associated with the switch between ion secretion and ion reabsorption by the distal ileac plexus. An unexpected finding is the presence of voltage-gated, ligand-gated and mechanosensitive ion channels, normally seen in excitable cells, in Malpighian tubules. Transcriptomic surveys indicate that these types of channels are also present in multiple other types of vertebrate and invertebrate epithelia, suggesting that they may play novel roles in epithelial cell signalling and regulation of epithelial ion transport.

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RNAi knockdown of CAPAr gene affects survival and fecundity of Bemisia tabaci (Gennadius)

Thakur

Jindal

2022

Journal of Asia-Pacific Entomology

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Voltage-gated calcium channels regulate K+ transport in the Malpighian tubules of the larval cabbage looper, Trichoplusia ni

Cited by 6 publications

References 52 publications

Adaptation of an arthropod predator to a challenging environment is associated with a loss of a genome‐wide plastic transcriptional response

Adaptation of an arthropod predator to a challenging environment is associated with a loss of a genome‐wide plastic transcriptional response

Blending physiology and RNAseq to provide new insights into regulation of epithelial transport: switching between ion secretion and reabsorption

RNAi knockdown of CAPAr gene affects survival and fecundity of Bemisia tabaci (Gennadius)

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