Aquiflexum lacus sp. nov., isolated from a lake sediment sample

Huang, Jiangen; Han, Ming-Xian; Fang, Bao‐Zhu; Yang, Jian; Xiao, Haiyi; Zhang, Xiying; Han, Jibin; Yu, Dongmei; Jiang, Hongchen; Li, Wenjun

doi:10.1007/s00203-021-02280-z

Cited by 9 publications

(11 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Strain A. lacus CUG 91378 T was isolated from Qinghai Lake which is the largest inland saltwater lake in China [4].…”

Section: Resultsmentioning

confidence: 99%

“…Strains LQ15W T and AIY15W T also contained MK-8 and MK-7 as their major respiratory quinone, which was a little different from the respiratory quinone profiles of A. aquatile Z0201 T , A. balticum DSM 16537 T , A. lacus CUG 91378 T and M. saccharolyticus AK6 T [2][3][4]. The major fatty acids of the tested strains were iso-C 15 : 0 (16.6 −22.9 %), summed feature 3 (5.1−9.0 %) and summed feature 9 (6.4−7.8%).…”

Section: Chemotaxonomic Analysismentioning

confidence: 92%

“…Strains LQ15W T , AIY15W T and A. aquatile Z0201 T were isolated from freshwater lakes, but A. balticum DSM 16537 T and A. lacus CUG 91378 T were isolated from saltwater environments. Strain A. lacus CUG 91378 T was isolated from Qinghai Lake which is the largest inland saltwater lake in China [4].…”

Section: Characteristics Of Genomes and Phylogenomic Analysismentioning

confidence: 99%

“…Since this genus was proposed, only two species were reported. Aquiflexum aquatile was isolated from lake water [3] and Aquiflexum lacus was isolated from a lake sediment sample [4]. In addition to the cultivated strains, many uncultured bacterium clone sequences of the genus Aquiflexum have been found in Tibetan lakes and aerosol, snow and soil samples from remote glaciated areas (Antarctica, Alps and Andes) [5, 6].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Proposal to reclassify Aquiflexum aquatile into a novel genus as Cognataquiflexum aquatile gen. nov., comb. nov., and description of Cognataquiflexum nitidum sp. nov. and Cognataquiflexum rubidum sp. nov., isolated from freshwater lakes on the Tibetan Plateau

Gao

Lü

Phurbu³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Thousands of lakes harbouring different characteristics (pH, salinity, temperature) are located on the Tibetan Plateau, and the mining of microbial resources inhabited in these lakes has great value. Two Gram-stain-negative, aerobic, rod-shaped, non-motile strains (LQ15WT and AIY15WT) were isolated from freshwater lakes on the Tibetan Plateau. Comparisons based on the 16S rRNA gene sequences showed that both strains LQ15WT and AIY15WT share 16S rRNA gene sequence similarities 98.4 % with Aquiflexum aquatile Z0201T, but only about 95.0 % with Aquiflexum balticum DSM 16537T. The 16S rRNA gene sequence similarity between strains LQ15WT and AIY15WT was 98.9 %. The phylogenetic tree reconstructed based on 16S rRNA gene sequences also showed that strains LQ15WT and AIY15WT take A. aquatile Z0201T as their closest neighbour and these three strains form a tight cluster. In the phylogenomic tree, the genus Aquiflexum was splited into two clusters by Mariniradius saccharolyticus . Strains LQ15WT, AIY15WT and A. aquatile Z0201T still formed a close cluster, and A. balticum DSM 16537T and Aquiflexum lacus CUG 91378T formed another cluster. The calculated OrthoANIu, average amino acid identity and digital DNA–DNA hybridization values among strains LQ15WT, AIY15WT, A. aquatile Z0201T, A. balticum DSM 16537T and A. lacus CUG 91378T were less than 91.0, 92.9 and 42.1 %, respectively. The major respiratory quinones of both strains LQ15WT and AIY15WT were MK-7 (32 %) and MK-8 (68 %), and their major fatty acids were iso-C15 : 0, C18 : 1 ω9c, summed feature 3 and summed feature 9. The predominant polar lipids of both strains were phosphatidylethanolamine, unidentified aminophospholipids, unidentified phospholipids and lipids. Strain AIY15WT also contained phosphatidylglycerol and unidentified glycolipid. Considering the distinct phylogenetic relationships and chemotaxonomic characteristics between strains A. aquatile Z0201T and A. balticum DSM 16537T, it is proposed to reclassify A. aquatile into a novel genus Cognataquiflexum gen. nov. as Cognataquiflexum aquatile comb. nov., and strains LQ15WT and AIY15WT should represent two independent novel species of the genus Cognataquiflexum, for which the names Cognataquiflexum nitidum sp. nov. (type strain: LQ15WT=CICC 24711T=JCM 34222T) and Cognataquiflexum rubidum sp. nov. (type strain: AIY15WT=CICC 24708T=JCM 34612T) are proposed.

show abstract

“…Strain A. lacus CUG 91378 T was isolated from Qinghai Lake which is the largest inland saltwater lake in China [4].…”

Section: Resultsmentioning

confidence: 99%

Section: Chemotaxonomic Analysismentioning

confidence: 92%

Section: Characteristics Of Genomes and Phylogenomic Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Proposal to reclassify Aquiflexum aquatile into a novel genus as Cognataquiflexum aquatile gen. nov., comb. nov., and description of Cognataquiflexum nitidum sp. nov. and Cognataquiflexum rubidum sp. nov., isolated from freshwater lakes on the Tibetan Plateau

Gao

Lü

Phurbu³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

“…[27]. Growth on MA, Reasoner’s 2A agar (R2A; BD), Luria–Bertani agar (LB), nutrient agar (NA; BD), tryptic soy agar (TSA; BD) and Oesterhelt–Stoeckenius medium (OSM) [8] was determined at 30 °C for 7 days. Growth in 2216 broth (MB; Difco) at different temperatures (4, 10, 20, 25, 30, 37, 45, 50 °C) and NaCl concentrations [0–2.5% (w/v) at 0.5% intervals and 3.0–10.0% at 1% intervals] was measured for 7 days.…”

Section: Phenotypic Characteristicsmentioning

confidence: 99%

Aquiflexum gelatinilyticum sp. nov., isolated from river water

Zhang

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Two Gram-stain-negative, strictly aerobic, rod-shaped, non-motile and non-gliding bacteria, designated as XJ19-10T and XJ19-11, were isolated from river water in Xinjiang Uygur Autonomous Region, PR China. Cells of these strains were catalase-, oxidase- and gelatinase-positive and contained carotenoids but no flexirubins. Growth occurred at 10–30 °C, pH 7.0–9.0 and with 0–2.5% (w/v) NaCl. On the basis of the results of 16S rRNA gene sequence and genome analyses, the two isolates represented members of the genus Aquiflexum , and the closest relative was Aquiflexum aquatile Z0201T with 16S rRNA gene sequence pairwise similarities of 97.9–98.1%. Furthermore, the average nucleotide identities and digital DNA–DNA hybridization identities between the two isolates and other relatives were all less than 82.9 and 28.2 %, respectively, all below the species delineation thresholds. The results of pan-genomic analysis indicated that the type strain XJ19-10T shared 2813 core gene clusters with other three type strains of members of the genus Aquiflexum , as well as having 623 strain-specific clusters. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine, an unidentified aminolipid and unidentified lipids. The predominant fatty acids (>10% of the total contents) were iso-C15 : 0, iso-C15 : 1G, iso-C17 : 0 3-OH and summed feature 9, and MK-7 was the respiratory quinone. On the basis of the results of phenotypic, physiological, chemotaxonomic and genotypic characterization, strains XJ19-10T and XJ19-11 are considered to represent a novel species, for which the name Aquiflexum gelatinilyticum sp. nov. is proposed. The type strain is XJ19-10T (=CGMCC 1.19385T =KCTC 92266T).

show abstract

Psychromicrobium Xiongbiense sp. nov., an Actinobacterium Isolated from Forest Soil

Zhu,

Wang,

et al. 2024

Curr Microbiol

View full text Add to dashboard Cite

Aquiflexum lacus sp. nov., isolated from a lake sediment sample

Cited by 9 publications

References 29 publications

Proposal to reclassify Aquiflexum aquatile into a novel genus as Cognataquiflexum aquatile gen. nov., comb. nov., and description of Cognataquiflexum nitidum sp. nov. and Cognataquiflexum rubidum sp. nov., isolated from freshwater lakes on the Tibetan Plateau

Proposal to reclassify Aquiflexum aquatile into a novel genus as Cognataquiflexum aquatile gen. nov., comb. nov., and description of Cognataquiflexum nitidum sp. nov. and Cognataquiflexum rubidum sp. nov., isolated from freshwater lakes on the Tibetan Plateau

Aquiflexum gelatinilyticum sp. nov., isolated from river water

Psychromicrobium Xiongbiense sp. nov., an Actinobacterium Isolated from Forest Soil

Contact Info

Product

Resources

About