Morpho-physiological and molecular mechanisms of phenotypic root plasticity for rice adaptation to water stress conditions

Lucob-Agustin, Nonawin; Kawai, Tsubasa; Kano‐Nakata, Mana; Suralta, Roel Rodriguez; Niones, Jonathan M.; Hasegawa, Tomomi; Inari-Ikeda, Mayuko; Yamauchi, Akira; Inukai, Yoshiaki

doi:10.1270/jsbbs.20106

Cited by 13 publications

(14 citation statements)

References 84 publications

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“…S-type LRs largely contribute to the hydraulic conductivity of the whole root system (representing water uptake ability) ( Watanabe et al, 2020 ), while L-type LRs expand the root network in soils by producing higher-order LRs including both S- and L-types. The plastic development of LRs plays an important role for the adaptation to soil water fluctuation of rice ( Suralta et al, 2018 ; Lucob-Agustin et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Auxin Distribution in Lateral Root Primordium Development Affects the Size and Lateral Root Diameter of Rice

et al. 2022

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Lateral roots (LRs) occupy a large part of the root system and play a central role in plant water and nutrient uptake. Monocot plants, such as rice, produce two types of LRs: the S-type (short and thin) and the L-type (long, thick, and capable of further branching). Because of the ability to produce higher-order branches, the L-type LR formation contributes to efficient root system expansion. Auxin plays a major role in regulating the root system development, but its involvement in developing different types of LRs is largely unknown. Here, we show that auxin distribution is involved in regulating LR diameter. Dynamin-related protein (DRP) genes were isolated as causative genes of the mutants with increased L-type LR number and diameter than wild-type (WT). In the drp mutants, reduced endocytic activity was detected in rice protoplast and LRs with a decreased OsPIN1b-GFP endocytosis in the protoplast. Analysis of auxin distribution using auxin-responsive promoter DR5 revealed the upregulated auxin signaling in L-type LR primordia (LRP) of the WT and the mutants. The application of polar auxin transport inhibitors enhanced the effect of exogenous auxin to increase LR diameter with upregulated auxin signaling in the basal part of LRP. Inducible repression of auxin signaling in the mOsIAA3-GR system suppressed the increase in LR diameter after root tip excision, suggesting a positive role of auxin signaling in LR diameter increase. A positive regulator of LR diameter, OsWOX10, was auxin-inducible and upregulated in the drp mutants more than the WT, and revealed as a potential target of ARF transcriptional activator. Therefore, auxin signaling upregulation in LRP, especially at the basal part, induces OsWOX10 expression, increasing LR diameter.

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Section: Introductionmentioning

confidence: 99%

Auxin Distribution in Lateral Root Primordium Development Affects the Size and Lateral Root Diameter of Rice

et al. 2022

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“…The production of L-type LRs is important for expanding the root system due to their ability to produce higher-order LRs. Plastic development of LRs plays an important role for the adaptation to soil water fluctuation in rice ( Suralta et al, 2018 ; Lucob-Agustin et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Rice Genotypes Express Compensatory Root Growth With Altered Root Distributions in Response to Root Cutting

et al. 2022

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Root systems play a pivotal role in water and nutrient uptake from soil. Lateral root (LR) growth is promoted to compensate for inhibited main root growth. Compensatory LR growth contributes to maintaining total root length (TRL) and hence water and nutrient uptake in compacted soils. However, it remains unclear how shoot and root phenotypic traits change during the compensatory growth and whether there are genotypic variations in compensatory root growth. This study analyzed shoot and root morphological traits of 20 rice genotypes, which includes mutants with altered root morphology, during the vegetative stage using a semihydroponic phenotyping system. The phenotyping experiment detected large variation in root and shoot traits among the 20 genotypes. Morphological changes induced by root cutting were analyzed in six selected genotypes with contrasting root system architecture. Root cutting significantly affected root distribution along vertical sections and among diameter classes. After root cutting, more roots distributed at shallower depth and thicker LRs developed. Furthermore, genotypes with deeper root growth without root cutting allocated more compensatory roots to deeper sections even after root cutting than the genotypes with shallower rooting. Due to the compensatory LR growth, root cutting did not significantly affect TRL, root dry weight (RDW), or shoot dry weight (SDW). To analyze the interaction between crown root (CR) number and compensatory root growth, we removed half of the newly emerged CRs in two genotypes. TRL of YRL38 increased at depth with CR number manipulation (CRM) regardless of root tip excision, which was attributed to an increase in specific root length (SRL), despite no change in RDW. Taken together, the tested rice genotypes exhibited compensatory root growth by changing root distribution at depth and in diameter classes. Reducing CR number promoted root development and compensatory growth by improving the efficiency of root development [root length (RL) per resource investment].

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“…Thus, it should be considered by breeders in selection for improving rice varieties adaptable for these stressed-growing environments. Depending on the characteristics of the rainfed environment, however, diverse and functional root plasticity traits contributing to a well-developed root system are manifested [reviewed in Suralta et al (2018); Lucob-Agustin et al (2021)]. The rice root system is composed of the parental roots, seminal and nodal roots (also called crown or shootborne roots), and their associated lateral roots (LRs).…”

Section: Introductionmentioning

confidence: 99%

Exploration of Candidate Genes Derived from SNPs Associated with Phenotypic Root Plasticity Using Philippine Rice Germplasm for Drought Adaptation

Lucob-Agustin¹,

Cruz²,

Lipio³

et al. 2021

Philipp J Sci

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Phenotypic root plasticity is a crucial trait for stable growth, adaptability, and productivity under abiotic-stressed growing environments. However, the genetic determinants and mechanisms controlling the trait are largely unknown, thus limiting its breeding application. We previously found 17 single nucleotide polymorphism (SNPs) significantly associated with phenotypic root plasticity traits – particularly, total root length (TRL), total nodal root length (TNRL), and total lateral root length (TLRL) – under moderate and severe drought using selected Philippine rice germplasm by employing the genome-wide association studies (GWAS) approach. Here, we present around 92 candidate genes using these SNP data. The genes were derived from various functional categories and drought intensity dependent. These were further narrowed via Gene Ontology and gene expression profile at root apical regions using databases to those whose putative functions were likely to be associated with root development in response to drought stress. Sixteen (16) more interesting known or novel genes were selected and discussed. When these genes are validated, they can help improve rice adaptation to water-limited environments.

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Morpho-physiological and molecular mechanisms of phenotypic root plasticity for rice adaptation to water stress conditions

Cited by 13 publications

References 84 publications

Auxin Distribution in Lateral Root Primordium Development Affects the Size and Lateral Root Diameter of Rice

Auxin Distribution in Lateral Root Primordium Development Affects the Size and Lateral Root Diameter of Rice

Rice Genotypes Express Compensatory Root Growth With Altered Root Distributions in Response to Root Cutting

Exploration of Candidate Genes Derived from SNPs Associated with Phenotypic Root Plasticity Using Philippine Rice Germplasm for Drought Adaptation

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