microRNA-mediated translation repression through GYF-1 and IFE-4 in <i>C. elegans</i> development

Mayya, Vinay K.; Flamand, Mathieu N.; Lambert, Alice M; Jafarnejad, Seyed Mehdi; Wohlschlegel, James A.; Sonenberg, Nahum

doi:10.1093/nar/gkab162

Cited by 34 publications

(40 citation statements)

References 64 publications

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“…To exert their regulatory roles, miRNAs are loaded into Argonaute (AGO) proteins to form a miRNA Induced Silencing Complex (miRISC), which ultimately associates with an effector protein GW182. miRISC binding to the target mRNA via partial sequence complementarity between a miRNA and 3′ UTR of mRNA triggers a series of gene silencing mechanisms including translation inhibition, decapping, and mRNA decay 3 , 4 .…”

Section: Introductionmentioning

confidence: 99%

Functional identification of microRNA-centered complexes in C. elegans

Hebbar

Panzade

Vashisht

et al. 2022

Sci Rep

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AbstractmicroRNAs (miRNAs) are crucial for normal development and physiology. To identify factors that might coordinate with miRNAs to regulate gene expression, we used 2′O-methylated oligonucleotides to precipitate Caenorhabditis elegans let-7, miR-58, and miR-2 miRNAs and the associated proteins. A total of 211 proteins were identified through mass-spectrometry analysis of miRNA co-precipitates, which included previously identified interactors of key miRNA pathway components. Gene ontology analysis of the identified interactors revealed an enrichment for RNA binding proteins, suggesting that we captured proteins that may be involved in mRNA lifecycle. To determine which miRNA interactors are important for miRNA activity, we used RNAi to deplete putative miRNA co-factors in animals with compromised miRNA activity and looked for alterations of the miRNA mutant phenotypes. Depletion of 25 of 39 tested genes modified the miRNA mutant phenotypes in three sensitized backgrounds. Modulators of miRNA phenotypes ranged from RNA binding proteins RBD-1 and CEY-1 to metabolic factors such as DLST-1 and ECH-5, among others. The observed functional interactions suggest widespread coordination of these proteins with miRNAs to ultimately regulate gene expression. This study provides a foundation for future investigations aimed at deciphering the molecular mechanisms of miRNA-mediated gene regulation.

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Section: Introductionmentioning

confidence: 99%

Functional identification of microRNA-centered complexes in C. elegans

Hebbar

Panzade

Vashisht

et al. 2022

Sci Rep

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“…In 2000, Reinhart et al found another important miRNA: let-7 posttranscriptional regulation: let-7 in wireworms. The discovery of lin-4 and let-7 kicked off the research on miRNA [ 5 , 6 ]. With the improvement of research methods and the increasing attention paid to miRNA, more and more miRNA molecules are constantly revealed in a variety of organisms and become biomarkers for the diagnosis of diseases and tumors.…”

Section: Introductionmentioning

confidence: 99%

Relationship between miR-338-3p and Clinicopathological Parameters, Prognosis, and STAT3 mRNA Expression in Nasopharyngeal Carcinoma

Wang

Ren

Pan

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Objective. To investigate the expression of miR-338-3p in nasopharyngeal carcinoma (NPC) and its relationship with STAT3 mRNA expression as well as their relationship with clinical pathological parameters and prognosis of patients. Methods. From September 2016 to September 2018, 71 patients with NPC were selected as the NPC group, and 71 samples of NPC tissues were collected during the operation. A total of 23 patients who underwent biopsy due to chronic nasopharyngitis were selected as the control group and 23 nasopharyngeal mucosal tissues were collected. The expressions of miR-338-3p and STAT3 mRNA in nasopharyngeal tissue of two groups were detected by real-time quantitative PCR, and the relationship between the two was analyzed. To collect clinical data of NPC patients and analyze the relationship between the expressions of miR-338-3p and STAT3 in NPC tissues and clinical pathological parameters of the patients, we followed up the patients with nasopharyngeal carcinoma for three years to observe the relationship between miR-338-3p, STAT3, and the prognosis of the patients. Results. The relative expression levels of miR-338-3p in nasopharyngeal tissues of the NPC group and the control group were 0.39 ± 0.05 and 1.01 ± 0.09, respectively ( P < 0.05). The relative expression levels of STAT3 mRNA in nasopharyngeal tissues of the NPC group and the control group were 3.82 ± 0.21 and 1.04 ± 0.11, respectively ( P > 0.05). miR-338-3p was negatively correlated with the relative expression of STAT3 mRNA in nasopharyngeal carcinoma (r = 0.038, P > 0.05). The expression of miR-338-3p was related to the age of the patient, clinical TNM stage, T stage, and distant metastasis (all P < 0.05). STAT3 expression was correlated with clinical TNM stage, T stage, and distant metastasis in our patient ( P < 0.05). The expressions of miR-338-3p and STAT3 in nasopharyngeal carcinoma tissues from different gender, histological type, N stage, M stage, and degree of differentiation showed no statistical differences ( P > 0.05). The survival rate of the group with low miR-338-3p expression was significantly lower than that of the group with high miR-338-3p expression ( P > 0.05). The survival rate of patients with the high STAT3 expression group was significantly lower than that of patients with the low STAT3 expression group ( P > 0.05). Conclusion. There is a negative correlation between the low expression of miR-338-3p and the high expression of STAT3 in NPC, which are all related to the TNM stage, T stage, and prognosis of the patient.

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“…In Drosophila , PatL1 associates with the Ddx6 (Me31B) and GIGYF proteins to repress translation ( Peter et al, 2019 ). The same interaction between PatL1 (PATR-1) and GIGYF (GYF-1) has also been reported in C. elegans , where GYF-1 represses translation of some developmental miRNA targets ( Mayya et al, 2021 ). Thus, promotion of decapping and decay or translational repression by PatL1 seems to depend on its specific interactions and on environmental and developmental contexts.…”

Section: Decapping Activatorsmentioning

confidence: 52%

Eukaryotic mRNA Decapping Activation

Vidya

2022

Front. Genet.

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The 5′-terminal cap is a fundamental determinant of eukaryotic gene expression which facilitates cap-dependent translation and protects mRNAs from exonucleolytic degradation. Enzyme-directed hydrolysis of the cap (decapping) decisively affects mRNA expression and turnover, and is a heavily regulated event. Following the identification of the decapping holoenzyme (Dcp1/2) over two decades ago, numerous studies revealed the complexity of decapping regulation across species and cell types. A conserved set of Dcp1/2-associated proteins, implicated in decapping activation and molecular scaffolding, were identified through genetic and molecular interaction studies, and yet their exact mechanisms of action are only emerging. In this review, we discuss the prevailing models on the roles and assembly of decapping co-factors, with considerations of conservation across species and comparison across physiological contexts. We next discuss the functional convergences of decapping machineries with other RNA-protein complexes in cytoplasmic P bodies and compare current views on their impact on mRNA stability and translation. Lastly, we review the current models of decapping activation and highlight important gaps in our current understanding.

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microRNA-mediated translation repression through GYF-1 and IFE-4 in C. elegans development

Cited by 34 publications

References 64 publications

Functional identification of microRNA-centered complexes in C. elegans

Functional identification of microRNA-centered complexes in C. elegans

Relationship between miR-338-3p and Clinicopathological Parameters, Prognosis, and STAT3 mRNA Expression in Nasopharyngeal Carcinoma

Eukaryotic mRNA Decapping Activation

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