Insulin signaling is an essential regulator of endometrial proliferation and implantation in mice

Sekulovski, Nikola; Whorton, Allison E; Shi, Mingxin; Hayashi, Kanako; MacLean, James A.

doi:10.1096/fj.202002448r

Cited by 24 publications

(19 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…INSR has been validated as a polycystic ovary syndrome risk locus using a very large, well-designed case-control GWAS ( Shi et al, 2012 ). Meanwhile, INSR has been confirmed to be necessary for optimal endometrial proliferation and implantation ( Sekulovski et al, 2021 ). Furthermore, INSR plays a crucial role in controlling adipose tissue development and adipocyte survival ( Boucher et al, 2012 ; Cignarelli et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic architecture and selection of Anhui autochthonous pig population revealed by whole genome resequencing

Zhang

Jiang

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

The genetic resources among pigs in Anhui Province are diverse, but their value and potential have yet to be discovered. To illustrate the genetic diversity and population structure of the Anhui pigs population, we resequenced the genome of 150 pigs from six representative Anhui pigs populations and analyzed this data together with the sequencing data from 40 Asian wild boars and commercial pigs. Our results showed that Anhui pigs were divided into two distinct types based on ancestral descent: Wannan Spotted pig (WSP) and Wannan Black pig (WBP) origins from the same ancestor and the other four populations origins from another ancestor. We also identified several potential selective sweep regions associated with domestication characteristics among Anhui pigs, including reproduction-associated genes (CABS1, INSL6, MAP3K12, IGF1R, INSR, LIMK2, PATZ1, MAPK1), lipid- and meat-related genes (SNX19, MSTN, MC5R, PRKG1, CREBBP, ADCY9), and ear size genes (MSRB3 and SOX5). Therefore, these findings expand the catalogue and how these genetic differences among pigs and this newly generated data will be a valuable resource for future genetic studies and for improving genome-assisted breeding of pigs and other domesticated animals.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic architecture and selection of Anhui autochthonous pig population revealed by whole genome resequencing

Zhang

Jiang

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

show abstract

“…Upregulated miR-328-3p is a biomarker in teratozoospermia and may decrease fertilization and embryo formation rate ( Corral-Vazquez et al, 2019 ). Moreover, for the three common DEmiRs between human and mouse sperm, it is reported that overexpression of miR-140-5p significantly inhibits cell proliferation and promotes cell apoptosis in chronic myeloid leukemia cells ( Geng et al, 2020 ; Jiao et al, 2020 ), while miR-30b-5p can promote cell proliferation and cell cycle progression in cancer ( Qin et al, 2017 ), and miR-148b-3p is correlated with fertility rate ( Chen et al, 2018 ; Sekulovski et al, 2021 ). More importantly, in our study, downregulation of miR-148b-3p, according to qRT-PCR analysis, in both human and mouse frozen-thawed sperm may still affect the expression of miR-148b-3p during subsequent embryonic development, leading to the significantly lower embryo formation rate.…”

Section: Discussionmentioning

confidence: 99%

Effect of Sperm Cryopreservation on miRNA Expression and Early Embryonic Development

Zhang

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Although sperm preservation is a common means of personal fertility preservation, its effects on embryonic development potential need further investigation. The purpose of this study was to identify key microRNA (miRNA) in cryopreserved sperm and determine the changes of these miRNAs and their target genes during embryonic development using cryopreserved sperm. Moreover, the embryonic development potential of cryopreserved sperm was estimated in assisted reproductive technology (ART), where key miRNAs and target genes were validated in sperm and subsequent embryos. Clinical data of embryonic development from cryopreserved sperm indicated a significant decrease in fertilization rate in both in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cases, as well as a reduction in blastocyst formation rate in ICSI cases. Meanwhile there was a significant increase in blocked embryo ratio of Day1, Day2, and Day3.5 embryos when frozen-thawed mouse sperm was used, compared with fresh mouse sperm, suggesting a potential negative effect of sperm cryopreservation on embryonic development. From frozen-thawed and fresh sperm in humans and mice, respectively, 21 and 95 differentially expressed miRNAs (DEmiRs) were detected. miR-148b-3p were downregulated in both human and mouse frozen-thawed sperm and were also decreased in embryos after fertilization using cryopreserved sperm. Target genes of miR-148b-3p, Pten, was identified in mouse embryos using quantitative real-time PCR (qRT-PCR) and Western blot (WB). In addition, common characters of cryopreservation of mouse oocytes compared with sperm were also detected; downregulation of miR-148b-3p was also confirmed in cryopreserved oocytes. In summary, our study suggested that cryopreservation of sperm could change the expression of miRNAs, especially the miR-148b-3p across humans and mice, and may further affect fertilization and embryo development by increasing the expression of Pten. Moreover, downregulation of miR-148b-3p induced by cryopreservation was conserved in mouse gametes.

show abstract

“…Furthermore, dual receptor ablation compromised decidualization, reduced endometrial thickness, and inhibited embryo implantation. Authors further observed decreased activation of ERK/MAPK signaling ( Sekulovski et al, 2021 ). Other pathological states, such as inflammation and obesity, cause insulin resistance by serine phosphorylation of the insulin receptor or insulin receptor substrate (IRS) proteins by TNF-α and PKCε ( Hotamisligil, 1999 ).…”

Section: Introductionmentioning

confidence: 93%

Endometrial Glucose Transporters in Health and Disease

Madunić

Karin-Kujundžić

Madunić

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Pregnancy loss is a frequent occurrence during the peri-implantation period, when there is high glucose demand for embryonic development and endometrial decidualization. Glucose is among the most essential uterine fluid components required for those processes. Numerous studies associate abnormal glucose metabolism in the endometrium with a higher risk of adverse pregnancy outcomes. The endometrium is incapable of synthesizing glucose, which thus must be delivered into the uterine lumen by glucose transporters (GLUTs) and/or the sodium-dependent glucose transporter 1 (SGLT1). Among the 26 glucose transporters (14 GLUTs and 12 SGLTs) described, 10 (9 GLUTs and SGLT1) are expressed in rodents and 8 (7 GLUTs and SGLT1) in the human uterus. This review summarizes present knowledge on the most studied glucose transporters in the uterine endometrium (GLUT1, GLUT3, GLUT4, and GLUT8), whose data regarding function and regulation are still lacking. We present the recently discovered SGLT1 in the mouse and human endometrium, responsible for controlling glycogen accumulation essential for embryo implantation. Moreover, we describe the epigenetic regulation of endometrial GLUTs, as well as signaling pathways included in uterine GLUT’s expression. Further investigation of the GLUTs function in different endometrial cells is of high importance, as numerous glucose transporters are associated with infertility, polycystic ovary syndrome, and gestational diabetes.

show abstract

Insulin signaling is an essential regulator of endometrial proliferation and implantation in mice

Cited by 24 publications

References 62 publications

Genetic architecture and selection of Anhui autochthonous pig population revealed by whole genome resequencing

Genetic architecture and selection of Anhui autochthonous pig population revealed by whole genome resequencing

Effect of Sperm Cryopreservation on miRNA Expression and Early Embryonic Development

Endometrial Glucose Transporters in Health and Disease

Contact Info

Product

Resources

About