Data on the uptake of CpG-loaded amino-dextran nanoparticles by antigen-presenting cells

Abstract

Cytosine-phosphate-guanine (CpG) oligonucleotides are commonly-used vaccine adjuvants to promote the activation of antigen-presenting cells (APCs). To mount an effective immune response, CpG needs to be internalized and bind to its endosomal Toll-like receptor 9 (TLR-9) inside the APCs. Using flow cytometry and fluorescence microscopy, this article presents the cellular uptake data of the amino-dextran nanoparticle (aDNP) and aDNP loaded with CpG immobilized on its surface by either electrostatic adsorption or covalent conjugation. The uptake of fluorescently-labelled aDNPs by murine splenic dendritic cells and macrophages was determined by flow cytometry and uptake by murine bone-marrow-derived dendritic cells was evaluated by fluorescence microscopy. The data presented in this paper correlates with the in vitro immune-stimulatory activity observed for the two different CpG loading methods in the research article “ Nanoparticle system based on amino-dextran as a drug delivery vehicle: immune-stimulatory CpG-oligonucleotide loading and delivery ” (Nguyen et al ., 2020) [1] . The data provide experimental evidence for a better understanding how the nanoparticle surface loading method of CpG influences the uptake of these nanoparticles by antigen-presenting cells as a step guide in the design of more effective vaccine formulations.

Dextran Formulations as Effective Delivery Systems of Therapeutic Agents

“…The in vivo studies have shown that both distribution and elimination of DEX depend on the molecular mass and overall charge of the polymer. Pharmacodynamically, the DEX-DDS have resulted in a prolonged effect, a low toxicity profile and a decreased immunogenicity of bioactive molecules [ 16 , 43 , 44 ].…”