Application of genetic cell-lineage tracing technology to study cardiovascular diseases

Sun, Xiaotong; Lyu, Lingxia; Zhong, Xingxing; Ni, Zhichao; Xu, Qingbo

doi:10.1016/j.yjmcc.2021.03.006

Cited by 4 publications

(2 citation statements)

References 149 publications

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“…TAM binds to the estrogen receptor (ER) to activate the expression of Cre recombinase, collectively referred to as the inducible Cre-loxP system, also known as the CreER system. After TAM was injected into mice, the complex formed by CreER and heat shock protein 90 (HSP90) (35,36) dissociated and nuclearly translocated, in turn activating the Cre-loxP system (Figure 1). In addition, several indicators were selected for specific single-cell lineage tracing, such as LacZ, GFP, red fluorescent protein (RFP), tdTomato, and Cherry.…”

Section: Principles and Approaches Of The Cre-loxp Systemmentioning

confidence: 99%

Cre-loxP-mediated genetic lineage tracing: Unraveling cell fate and origin in the developing heart

Wang

Chen²,

Wang³

et al. 2023

Front. Cardiovasc. Med.

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The Cre-loxP-mediated genetic lineage tracing system is essential for constructing the fate mapping of single-cell progeny or cell populations. Understanding the structural hierarchy of cardiac progenitor cells facilitates unraveling cell fate and origin issues in cardiac development. Several prospective Cre-loxP-based lineage-tracing systems have been used to analyze precisely the fate determination and developmental characteristics of endocardial cells (ECs), epicardial cells, and cardiomyocytes. Therefore, emerging lineage-tracing techniques advance the study of cardiovascular-related cellular plasticity. In this review, we illustrate the principles and methods of the emerging Cre-loxP-based genetic lineage tracing technology for trajectory monitoring of distinct cell lineages in the heart. The comprehensive demonstration of the differentiation process of single-cell progeny using genetic lineage tracing technology has made outstanding contributions to cardiac development and homeostasis, providing new therapeutic strategies for tissue regeneration in congenital and cardiovascular diseases (CVDs).

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Section: Principles and Approaches Of The Cre-loxp Systemmentioning

confidence: 99%

Cre-loxP-mediated genetic lineage tracing: Unraveling cell fate and origin in the developing heart

Wang

Chen²,

Wang³

et al. 2023

Front. Cardiovasc. Med.

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“…This method is often combined with lineage tracing techniques where the labeled bone marrow is grafted into wild-type or sex-mismatched mice. These studies show that bone marrow derived cells mainly give rise to inflammatory cells in the plaques and do not transdifferentiate into SMCs or ECs ( 51 ). However, supportive scRNA-seq evidence is lacking.…”

Section: Current Knowledge About Cellular Composition: Insights From ...mentioning

confidence: 99%

The Applications of Single-Cell RNA Sequencing in Atherosclerotic Disease

Slenders

Tessels

Laan

et al. 2022

Front. Cardiovasc. Med.

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Atherosclerosis still is the primary cause of death worldwide. Our characterization of the atherosclerotic lesion is mainly rooted in definitions based on pathological descriptions. We often speak in absolutes regarding plaque phenotypes: vulnerable vs. stable plaques or plaque rupture vs. plaque erosion. By focusing on these concepts, we may have oversimplified the atherosclerotic disease and its mechanisms. The widely used definitions of pathology-based plaque phenotypes can be fine-tuned with observations made with various -omics techniques. Recent advancements in single-cell transcriptomics provide the opportunity to characterize the cellular composition of the atherosclerotic plaque. This additional layer of information facilitates the in-depth characterization of the atherosclerotic plaque. In this review, we discuss the impact that single-cell transcriptomics may exert on our current understanding of atherosclerosis.

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