Complete Genome Sequence of the Bacterial Component of Mysorin Biopreparation

Afonin, Alexey М.; Gribchenko, Emma S.; Akhtemova, Gulnar A.; Laktionov, Yu.V.; Kozhemyakov, A. P.; Zhukov, V. A.

doi:10.1128/mra.01287-20

Cited by 2 publications

(2 citation statements)

References 13 publications

(11 reference statements)

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“…Although they possess countless abilities related to plant growth promotion, only a few actinomycete-based biofertilizers are currently commercialized. Mizorin R is a biofertilizer based on actinomycetes used in corn, beetroot, and mustard (107). Bamil R and Omug R are also microbial inoculants based on actinomycetes (Micrococcus spp.…”

Section: Nutrient Availabilitymentioning

confidence: 99%

The Potential Use of Actinomycetes as Microbial Inoculants and Biopesticides in Agriculture

et al. 2022

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The use of fertilizers and chemical pesticides promotes significant improvements in crop development, but some problems and risks associated with them limit their application. An alternative is using biological inputs based on microorganisms, increasing production while combining efficiency and sustainability. Actinomycetes are a group of bacteria belonging to the phylum Actinobacteria, recently re-named Actinobacteriota. They represent important microbial communities in the soils with increasing agricultural applications, especially in the biological control of insect-pest and plant disease and in plant growth promotion. Studies report their promising use as microbiological inoculants by exploring mechanisms to improve plant development, such as biological nitrogen fixation, phosphate solubilization, production of phytohormones, and other biocompounds. In addition, many species produce metabolic pathways that generate high-value antibiotics, extracellular enzymes and secondary metabolites other than antimicrobials, with potential in the control of phytopathogenic fungi, insects, and nematodes. These actinomycetes could be used to formulate novel bioinoculants composed of spores and/or mycelium. Considering that the research in this field is up-and-coming, with significant economic and environmental impacts in the future, this review aims to group the most relevant works that explore the biodiversity of actinomycetes, helping to develop inoculants and biodefensives for more productive and conscious agriculture.

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Section: Nutrient Availabilitymentioning

confidence: 99%

The Potential Use of Actinomycetes as Microbial Inoculants and Biopesticides in Agriculture

et al. 2022

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“…Fresh roots of A. schoenoprasum colonized by R. irregularis were surface-disinfected as described by Cranenbrouck et al (2005), cut into 0.5-1 cm segments and used as AM fungal inoculum in this study (0.2 g of inoculum per plant into wells made in the sand before planting). Finally, in variants with PGPB inoculation, 2 ml of water suspension of Arthrobacter mysorens 7 strain (accession number in The Russian Collection of Agricultural Microorganisms (RCAM) (ARRIAM, St. Petersburg) RCAM 01094 (Afonin et al, 2021)) in concentration 5 * 10 6 CFU/ml were poured under each planted seedling. This strain is an active component of 'Mysorin' biopreparation that was previously described as possessing plant growth promoting qualities and was shown to enhance the effect of other biopreparations containing R. leguminosarum (Kozhemyakov et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

Transcriptomic analysis of sym28 and sym29 supernodulating mutants of pea (Pisum sativum L.) under complex inoculation with beneficial microorganisms

Zhukov

Zorin

Zhernakov

et al. 2021

BioComm

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The garden pea (Pisum sativum L.), like most members of Fabaceae family, is capable of forming symbioses with beneficial soil microorganisms such as nodule bacteria (rhizobia), arbuscular mycorrhizal (AM) fungi and plant growth promoting bacteria (PGPB). The autoregulation of nodulation (AON) system is known to play an important role in controlling both the number of nodules and the level of root colonization by AM via root-to-shoot signaling mediated by CLAVATA/ESR-related (CLE) peptides and their receptors. In the pea, mutations in genes Sym28 (CLV2-like) and Sym29 (CLV1-like), which encode receptors for CLE peptides, lead to the supernodulation phenotype, i.e., excessive nodule formation. The aim of the present study was to analyze the response of pea cv. ‘Frisson’ (wild type) and mutants P64 (sym28) and P88 (sym29) to complex inoculation with rhizobia, AM fungi and PGPB, with regard to biomass accumulation, yield and transcriptomic alterations. The plants were grown in quartz sand for 2 and 4 weeks after inoculation with either rhizobia (Rh) or complex inoculation with Rh + AM, Rh + PGPB, and Rh+AM+PGPB, and the biomass and yield were assessed. Transcriptome sequencing of whole shoots and roots was performed using a modified RNAseq protocol named MACE (Massive Analysis of cDNA Ends). In the experimental conditions, P88 (sym29) plants demonstrated the best biomass accumulation and yield, as compared to the wild type and P64 (sym28) plants, whereas P64 (sym28) had the lowest rate of biomass and seed yield. The transcriptome analysis showed that both supernodulating mutants more actively responded to biotic and abiotic factors than the wild-type plants and demonstrated increased expression of genes characteristic to late stages of nodule development. The roots of P64 (sym28) plants responded to AM+Rh treatment with upregulation of genes encoding plastid proteins, which can be connected with the activation of carotenoid biosynthesis (namely, the non-mevalonate pathway that takes place in root plastids). The more active response to symbionts in P88 (sym29) plants, as compared to cv. ‘Frisson’, was associated with counterregulation of transcripts involved in chloroplast functioning and development in leaves, which accompanies successful plant development in symbiotic conditions. Finally, the effect of retardation of plant aging upon mycorrhization on a transcriptomic level was recorded for cv. ‘Frisson’ but not for P64 (sym28) and P88 (sym29) mutants, which points towards its possible connection with the AON system. The results of this work link the plant’s autoregulation with the responsiveness to inoculation with beneficial soil microorganisms.

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Complete Genome Sequence of the Bacterial Component of Mysorin Biopreparation

Cited by 2 publications

References 13 publications

The Potential Use of Actinomycetes as Microbial Inoculants and Biopesticides in Agriculture

The Potential Use of Actinomycetes as Microbial Inoculants and Biopesticides in Agriculture

Transcriptomic analysis of <em>sym28</em> and <em>sym29</em> supernodulating mutants of pea (<em>Pisum sativum</em> L.) under complex inoculation with beneficial microorganisms

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