Light‐induced local gene expression in primary chick cell culture system

Kitajima, Keiichi; Kawahira, Naofumi; Lee, Sang Woo; Tamura, Koji; Morishita, Yoshihiro; Ohtsuka, Daisuke

doi:10.1111/dgd.12721

Cited by 2 publications

(2 citation statements)

References 39 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the use of optogenetic approaches is not limited to studying neural networks. Optogenetic approaches utilising photoreceptors [150] allow for the control of the transcription of target genes using light [3,[151][152][153][154]. The photoreceptors used in optogenetics can be divided into several groups: blue-light-utilising flavoproteins, cryptochromes, and xanthopsins; opsins utilising blue, green, and red light; and far-red-and near-infrared (NIR) light-sensing phytochromes [150].…”

Section: Optogenetic Approachesmentioning

confidence: 99%

Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals

Motorina,

Galimova,

Battulina

et al. 2024

IJMS

View full text Add to dashboard Cite

At present, there are a variety of different approaches to the targeted regulation of gene expression. However, most approaches are devoted to the activation of gene transcription, and the methods for gene silencing are much fewer in number. In this review, we describe the main systems used for the targeted suppression of gene expression (including RNA interference (RNAi), chimeric transcription factors, chimeric zinc finger proteins, transcription activator-like effectors (TALEs)-based repressors, optogenetic tools, and CRISPR/Cas-based repressors) and their application in eukaryotes—plants and animals. We consider the advantages and disadvantages of each approach, compare their effectiveness, and discuss the peculiarities of their usage in plant and animal organisms. This review will be useful for researchers in the field of gene transcription suppression and will allow them to choose the optimal method for suppressing the expression of the gene of interest depending on the research object.

show abstract

Section: Optogenetic Approachesmentioning

confidence: 99%

Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals

Motorina,

Galimova,

Battulina

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

“…The LightOn system has been shown to be able to control transgene expression in HEK293 cells and mice [14]. In addition, it has been used to control the cell fate of neural progenitor cells and chick-limb mesenchyme cells [17,18], study cell ablation in zebrafish [19], regulate blood-glucose homeostasis in type 1 diabetic mice [20] and target a potential breast cancer tumor by a light-switchable gene expression system encapsulated in a nanoparticle delivery system [20].…”

Section: Introductionmentioning

confidence: 99%

OPTO-BLUE: An Integrated Bidirectional Optogenetic Lentiviral Platform for Controlled Light-Induced Gene Expression

Arancibia,

Pol,

Vargas-Fernández

et al. 2023

IJMS

View full text Add to dashboard Cite

Regulated systems for transgene expression are useful tools in basic research and a promising platform in biomedicine due to their regulated transgene expression by an inducer. The emergence of optogenetics expression systems enabled the construction of light-switchable systems, enhancing the spatial and temporal resolution of a transgene. The LightOn system is an optogenetic tool that regulates the expression of a gene of interest using blue light as an inducer. This system is based on a photosensitive protein (GAVPO), which dimerizes and binds to the UASG sequence in response to blue light, triggering the expression of a downstream transgene. Previously, we adapted the LightOn system to a dual lentiviral vector system for neurons. Here, we continue the optimization and assemble all components of the LightOn system into a single lentiviral plasmid, the OPTO-BLUE system. For functional validation, we used enhanced green fluorescent protein (EGFP) as an expression reporter (OPTO-BLUE-EGFP) and evaluated the efficiency of EGFP expression by transfection and transduction in HEK293-T cells exposed to continuous blue-light illumination. Altogether, these results prove that the optimized OPTO-BLUE system allows the light-controlled expression of a reporter protein according to a specific time and light intensity. Likewise, this system should provide an important molecular tool to modulate gene expression of any protein by blue light.

show abstract

Light‐induced local gene expression in primary chick cell culture system

Cited by 2 publications

References 39 publications

Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals

Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals

OPTO-BLUE: An Integrated Bidirectional Optogenetic Lentiviral Platform for Controlled Light-Induced Gene Expression

Contact Info

Product

Resources

About