MICA A5.1 homozygous genotype is associated with a risk for early-onset oral cancer

Tani, Ryotaro; Ito, Nanako; Matsui, Kensaku; Yamasaki, Sachiko; Hamada, Atsuko; Tokumaru, Koichiro; Toratani, Shigeaki; Okamoto, Tomoyoshi

doi:10.1016/j.oraloncology.2021.105256

Cited by 3 publications

(3 citation statements)

References 22 publications

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“…We recently reported that OSCC patients with the MICA A5.1 allele have a higher survival rate than those with other alleles as a result of the NKG2D receptor-mediated activation of NK cell signaling. Additionally, we demonstrated that the prognosis for patients with the A5.1 homozygous genotype was significantly better than that for patients with the other MICA genotypes [ 19 ]. Consequently, genetic polymorphisms of MICA and CD45RO + /TILs may serve as potential prognostic factors for OSCC patients.…”

Section: Discussionmentioning

confidence: 99%

“…Paraffin-embedded tissues were cut at 4–6 μm thickness and immuno-stained to evaluate the expression of CD3, CD8, CD45RO, Granzyme B, and MICA in every OSCC sample [ 19 ]. Subsequently, the sections were exposed to protein block 5% normal horse serum (Thermo Fisher Scientific, Waltham, MA, USA) and 2% normal goat serum (Thermo Fisher Scientific) and incubated overnight at 4°C with mouse monoclonal anti-MICA (Nichirei Biosciences, Inc., Tokyo, Japan), mouse monoclonal anti-CD3 (PS1, Nichirei Biosciences, Inc.), mouse monoclonal anti-CD8 (C8/144B, Nichirei Biosciences, Inc.), mouse monoclonal anti-UCHL (UCHL1, Nichirei Biosciences, Inc.) for CD45RO, and mouse monoclonal anti-Granzyme B (GrB-7, Nichirei Biosciences, Inc.), respectively [ 20 , 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…We recently reported that OSCC patients with the MICA A5.1 allele have a higher survival rate than those with other alleles due to NKG2D receptor-mediated NK cell signaling activation [ 19 ]. There have been no reports on the relationship between cancer cell MICA expression and TILs to date.…”

Section: Introductionmentioning

confidence: 99%

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Tumor-Infiltrating CD45RO+ Memory Cells Are Associated with Favorable Prognosis in Oral Squamous Cell Carcinoma Patients

Ito

Yamasaki

Shintani

et al. 2023

Cancers

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Background: Tumor-infiltrating lymphocytes (TILs) have been used to predict the prognosis of solid tumors. In this study, we investigated which molecules in TILs play a role in the prognosis of patients with oral squamous cell carcinoma (OSCC). Methods: In a retrospective case-control study, we immunohistochemically evaluated the expression of CD3, CD8, CD45RO, Granzyme B, and the major histocompatibility complex class I chain-related molecule A (MICA) of the histocompatibility complex as predictors of prognosis in 33 patients with OSCC. The patients were classified as TILsHigh or TILsLow according to the number of TILs for each molecule in the central tumor (CT) and invasive margin (IM). Furthermore, MICA expression scores were determined based on the intensity of the staining. Results: CD45RO+/TIL in the nonrecurrent group were significantly higher than those in the recurrent group in the CT and IM areas (p < 0.05). The disease-free survival/overall survival rate of the CD45RO+/TILsLow group in the CT and IM areas and the Granzyme B+/TILsLow group in the IM area was significantly lower than that of the CD45RO+/TILsHigh group and the Granzyme B+/TILsHigh group, respectively (p < 0.05). Furthermore, the MICA expression score of tumors around the CD45RO+/TILsHigh group was significantly higher than that of the CD45RO+/TILsLow group (p < 0.05). Conclusions: A high ratio of CD45RO-expressing TILs was associated with a disease-free/overall survival improvement in OSCC patients. Furthermore, the number of TILs that express CD45RO was associated with the expression of MICA in tumors. These results suggest that CD45RO-expressing TILs are useful biomarkers for OSCC.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Tumor-Infiltrating CD45RO+ Memory Cells Are Associated with Favorable Prognosis in Oral Squamous Cell Carcinoma Patients

Ito

Yamasaki

Shintani

et al. 2023

Cancers

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The role of non-classical and chain-related human leukocyte antigen polymorphisms in laryngeal squamous cell carcinoma

Ormandjieva

Yordanov²,

Stoyanov

et al. 2023

Mol Biol Rep

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Background:Laryngeal cancer is one of the most common head and neck malignancies worldwide.Laryngeal squamous cell carcinoma (LSCC) is the major pathological subtype of laryngeal cancer. LSCC originates from the epidermal keratinocytes and is characterized by impaired keratinization. It has been shown that alterations of the expression of non-classical human leukocyte antigens (HLA) and the chainrelated MIC molecules by malignant cells can lead to escape from the immune system control and certain allele variants may participate in immune editing and therefore be associated with modulation of cancer risk. The aim of the present study was to investigate the role of non-classical HLA class Ib and chainrelated MIC polymorphisms, determined at the allelic level by next-generation sequencing (NGS), in patients from the Bulgarian population, diagnosed with LSCC.Materials and methods:In the present study DNA samples from 48 patients with LSCC were used. Data was compared to 68 healthy controls analysed in previous studies. HLA genotyping was performed by using the AlloSeq Tx17 early pooling protocol and the library preparation AlloSeq Tx17 kit (CareDx).Sequencing was performed on MiniSeq sequencing platform (Illumina) and HLA genotypes were assigned with the AlloSeq Assign analysis software v1.0.3 (CareDx) and the IPD-IMGT/HLA database 3.45.1.2. Results:The HLA disease association tests revealed a statistically signi cant predisposing association of HLA-F*01:01:02 (P=0.0103, OR=24.0194) with LSCC, while HLA-F*01:01:01 (P=8.21e-04, OR=0.0485) has a possible protective association. Additionally we observed several haplotypes with statistically signi cant protective and predisposing associations. The strongest association was observed for F*01:01:01-H*01:01:01 (P=0.0054, haplotype score=-2.7801). Conclusion:Our preliminary study suggests the involvement of HLA class Ib in cancer development and the possible role of the shown alleles as biomarkers of development of LSCC.

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The Role of Non-classical and Chain-Related Human Leukocyte Antigen Polymorphisms in Laryngeal Squamous Cell Carcinoma

Ormandjieva

Yordanov

Stoyanov

et al. 2023

Preprint

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Background:Laryngeal cancer is one of the most common head and neck malignancies worldwide. Laryngeal squamous cell carcinoma (LSCC) is the major pathological subtype of laryngeal cancer. LSCC originates from the epidermal keratinocytes and is characterized by impaired keratinization. It has been shown that alterations of the expression of non-classical human leukocyte antigens (HLA) and the chain-related MIC molecules by malignant cells can lead to escape from the immune system control and certain allele variants may participate in immune editing and therefore be associated with modulation of cancer risk. The aim of the present study was to investigate the role of non-classical HLA class Ib and chain-related MIC polymorphisms, determined at the allelic level by next-generation sequencing (NGS), in patients from the Bulgarian population, diagnosed with LSCC. Materials and methods:In the present study DNA samples from 48 patients with LSCC were used. Data was compared to 68 healthy controls analysed in previous studies. HLA genotyping was performed by using the AlloSeq Tx17 early pooling protocol and the library preparation AlloSeq Tx17 kit (CareDx). Sequencing was performed on MiniSeq sequencing platform (Illumina) and HLA genotypes were assigned with the AlloSeq Assign analysis software v1.0.3 (CareDx) and the IPD-IMGT/HLA database 3.45.1.2. Results:The HLA disease association tests revealed a statistically significant predisposing association of HLA-F*01:01:02 (P=0.0103, OR=24.0194) with LSCC, while HLA-F*01:01:01 (P=8.21e-04, OR=0.0485) has a possible protective association. Additionally we observed several haplotypes with statistically significant protective and predisposing associations. The strongest association was observed for F*01:01:01-H*01:01:01 (P=0.0054, haplotype score=-2.7801). Conclusion:Our preliminary study suggests the involvement of HLA class Ib in cancer development and the possible role of the shown alleles as biomarkers of development of LSCC.

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MICA A5.1 homozygous genotype is associated with a risk for early-onset oral cancer

Cited by 3 publications

References 22 publications

Tumor-Infiltrating CD45RO+ Memory Cells Are Associated with Favorable Prognosis in Oral Squamous Cell Carcinoma Patients

Tumor-Infiltrating CD45RO+ Memory Cells Are Associated with Favorable Prognosis in Oral Squamous Cell Carcinoma Patients

The role of non-classical and chain-related human leukocyte antigen polymorphisms in laryngeal squamous cell carcinoma

The Role of Non-classical and Chain-Related Human Leukocyte Antigen Polymorphisms in Laryngeal Squamous Cell Carcinoma

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