Root developmental responses to phosphorus nutrition

Liu, Dong

doi:10.1111/jipb.13090

Cited by 99 publications

(53 citation statements)

References 204 publications

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“…This result suggests that low-nutrient may be beneficial to the growth of root. Previous studies have reported that plants under nitrogen and phosphorus limitation increase resource allocation to root growth relative to shoot growth (Schneider et al 2021;Liu 2021). To cope with nitrogen and phosphorus deficiency, a typical response is the inhibition of photosynthesis and an increase in the root/shoot ratio due to a decrease in shoot growth and to an increase in the allocation of carbon from shoots to roots (Hermans et al 2006;López-Arredondo et al 2014;Schneider et al 2021).…”

Section: Discussionmentioning

confidence: 99%

De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.)

Zhao

Zhang

Liu

et al. 2021

Physiol Mol Biol Plants

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The legume plant alfalfa (Medicago sativa L.) is a widely cultivated perennial forage due to its high protein content, palatability, and strong adaptability to diverse agro-ecological zones. Alfalfa is a self-incompatible cross-pollinated autotetraploid species with tetrasomic inheritance. Therefore, maintaining excellent traits through seed reproduction is a prime challenge in alfalfa. However, the cutting propagation technology could enable consistent multiplication of quality plants that are genetically identical to the parent plant. The current study aimed to develop a simple, cost-effective, reproducible, and efficient hydroponic cutting method to preserve alfalfa plants and for molecular research. In this study, alfalfa landrace ‘Wudi’ was grown in hydroponics for 30 days and used as source material for cuttings. The top, middle and bottom sections of its stem were used as cuttings. The rooting rate, root length, and stem height of the different stem sections were compared to determine the best segment for alfalfa propagation in four nutrient treatments (HM, HM + 1/500H, HM + 1/1000H and d HM + 1/2000H). After 21 days of culture, the rooting rates of all the three stem types under four cutting nutrient solutions were above 78%. The rooting rate of the middle and bottom parts in HM + 1/1000 H and HM + 1/2000 H nutrient solutions reached more than 93%, with a higher health survey score (> 4.70). In conclusion, this study developed a de novo cutting propagation method that can be used to conserve and propagate germplasm in breeding programs and research. This method is a new report on the cutting propagation of alfalfa by hydroponics, which could supplement the existing cutting propagation methods.

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Section: Discussionmentioning

confidence: 99%

De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.)

Zhao

Zhang

Liu

et al. 2021

Physiol Mol Biol Plants

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“…The first role can be summarized as biofertilizer. Phosphorus is essential for plant growth; however, its bioavailable form is often present in very low amounts (Liu 2021). The transformation of phosphorus in minerals and organic sources to their bioavailable forms occurs through secretion of organic acid in combination with acid phosphatases and phytases (Kang et al 2014;Martínez-Viveros et al 2010).…”

Section: Whole-cell Systems For Recombinant Production Of P450s In P Megateriummentioning

confidence: 99%

The “beauty in the beast”—the multiple uses of Priestia megaterium in biotechnology

Biedendieck

Knuuti

Jahn

2021

Appl Microbiol Biotechnol

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Over 30 years, the Gram-positive bacterium Priestia megaterium (previously known as Bacillus megaterium) was systematically developed for biotechnological applications ranging from the production of small molecules like vitamin B12, over polymers like polyhydroxybutyrate (PHB) up to the in vivo and in vitro synthesis of multiple proteins and finally whole-cell applications. Here we describe the use of the natural vitamin B12 (cobalamin) producer P. megaterium for the elucidation of the biosynthetic pathway and the subsequent systematic knowledge-based development for production purposes. The formation of PHB, a natural product of P. megaterium and potential petro-plastic substitute, is covered and discussed. Further important biotechnological characteristics of P. megaterium for recombinant protein production including high protein secretion capacity and simple cultivation on value-added carbon sources are outlined. This includes the advanced system with almost 30 commercially available expression vectors for the intracellular and extracellular production of recombinant proteins at the g/L scale. We also revealed a novel P. megaterium transcription-translation system as a complementary and versatile biotechnological tool kit. As an impressive biotechnology application, the formation of various cytochrome P450 is also critically highlighted. Finally, whole cellular applications in plant protection are completing the overall picture of P. megaterium as a versatile giant cell factory. Key points • The use of Priestia megaterium for the biosynthesis of small molecules and recombinant proteins through to whole-cell applications is reviewed. • P. megaterium can act as a promising alternative host in biotechnological production processes.

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“…ROP6 exists mainly in inactive form and is degraded through 26S proteasome pathway, which relieves the inhibitory regulation of PHT1;1 and PHT1;4, subsequently facilitating Pi uptake, eventually enhancing the tolerance of plants to Pi-deficient conditions. artifact under laboratory conditions, this phenomenon could still be regarded as an indicator of the degree of plant response to Pi deficiency (Zheng et al, 2019;Liu, 2021). Meanwhile the extent of Pi deficiency tolerance of plant is always coupled with their sensitivity to As(V) (Shin et al, 2004;Castrillo et al, 2013), and it is well reflected on rop6 mutant (Figure 1).…”

Section: Rop6 Is a Negative Regulator Of The Pi Deficiency Signaling Pathwaymentioning

confidence: 99%

“…The Pi uptake rate of the pht1;1pht1;4 double mutant is reduced by more than 50% at low Pi and close to 75% at high Pi compared with the wild type (Shin et al, 2004). The inhibition of Pi deficiency-induced primary root (PR) growth is a typical characteristic observed under light in Petri dishes, which is elucidated to be triggered by blue light through malate-mediated photo-Fenton reaction (Zheng et al, 2019;Gao et al, 2021;Liu, 2021). Arsenate (As(V)), structurally analogous to Pi, is harmful to nearly all organisms.…”

Section: Introductionmentioning

confidence: 99%

GTPase ROP6 negatively modulates phosphate deficiency through inhibition of PHT1;1 and PHT1;4 in Arabidopsis thaliana

Gao

Wang

Zhang

et al. 2021

JIPB

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Phosphorus, an essential macroelement for plant growth and development, is a major limiting factor for sustainable crop yield. The Rho of plant (ROP) GTPase is involved in regulating multiple signal transduction processes in plants, but potentially including the phosphate deficiency signaling pathway remains unknown. Here, we identified that the rop6 mutant exhibited a dramatic tolerant phenotype under Pi-deficient conditions, with higher phosphate accumulation and lower anthocyanin content. In contrast, the rop6 mutant was more sensitive to arsenate (As(V)) toxicity, the analog of Pi. Immunoblot analysis displayed that the ROP6 protein was rapidly degraded through ubiquitin/26S proteasome pathway under Pideficient conditions. In addition, pull-down assay using GST-RIC1 demonstrated that the ROP6 activity was decreased obviously under Pi-deficient conditions. Strikingly, protein-protein interaction and two-voltage clamping assays demonstrated that ROP6 physically interacted with and inhibited the key phosphate uptake transporters PHT1;1 and PHT1;4 in vitro and in vivo. Moreover, genetic analysis showed that ROP6 functioned upstream of PHT1;1 and PHT1;4. Thus, we conclude that GTPase ROP6 modulates the uptake of phosphate by inhibiting the activities of PHT1;1 and PHT1;4 in Arabidopsis.

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Root developmental responses to phosphorus nutrition

Cited by 99 publications

References 204 publications

De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.)

De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.)

The “beauty in the beast”—the multiple uses of Priestia megaterium in biotechnology

GTPase ROP6 negatively modulates phosphate deficiency through inhibition of PHT1;1 and PHT1;4 in Arabidopsis thaliana

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