Complete Genome Sequence of Bovine Adenovirus Type 7 Strain Fukuroi, Isolated from a Cow with Respiratory Disease

Kumagai, Asuka; Kajikawa, Sayo; Miyazaki, Akira; Hatama, Shinichi

doi:10.1128/mra.00055-21

Cited by 6 publications

(6 citation statements)

References 7 publications

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“…Phylogenetic analysis of the newly derived full-length sequence of BAdV-7 strain S427/18 agrees with previous phylogenetic analyses of sequences derived from the hexon, DNA polymerase and penton base genes [ 3 , 4 , 20 ]. These studies have reported that the BadV-7 and OAdV-7 predicted aa sequences of the DNA polymerase, penton base and hexon genes share sequence identity of 67, 84, and 86% respectively.…”

Section: Discussionsupporting

confidence: 85%

“…BAdV-7 naturally infects cattle, but also has been detected in sheep in Australia [ 9 , 19 ] and is most closely antigenically related to ovine Adenovirus type 7 [ 6 , 12 , 19 ]. However, the complete genome sequences of the “Fukuroi” strain and the derivate live attenuated vaccine strain “TS-GT were only recently reported [ 18 , 20 ]. This is augmented by a complete sequence for the SD18-74 strain which was isolated from pooled lung tissue from two 2-week-old calves from South Dakota, USA, that succumbed to acute enteric disease in 2018 [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in Germany

Jesse

Ciurkiewicz

Siesenop³

et al. 2022

Virol J

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Bovine adenovirus 7 (BAdV-7) is an unclassified member of the genus Atadenovirus with a worldwide distribution and has been reported to induce clinical disease of varying severity in infected cattle, ranging from asymptomatic infections to severe enteric or respiratory disease. In this study, we used next-generation sequencing to obtain the first complete genome sequence of a European strain of BadV-7, from pooled spleen and liver tissue obtained from a deceased newborn Limousin calf. Histopathological analysis and electron microscopy showing systemic lesions in multiple organs with intranuclear amphophilic inclusions observed in endothelial cells in multiple peripheral tissues. Virus isolation was readily achieved from tissue homogenate using bovine esophagus cells (KOP-R), a strategy which should facilitate future in vitro or in vivo BAdV-7 studies. Phylogenetic analysis of available genome sequences of BAdV-7 showed that the newly identified strain groups most closely with a recent BAdV-7 strain, SD18-74, from the USA, confirming that this newly identified strain is a member of the Atadenovirus genus. The fiber gene was found to be highly conserved within BAdV-7 strains but was highly divergent in comparison to Ovine adenovirus 7 (OAdV-7) (39.56% aa sequence identity). Furthermore, we report a variable region of multiple tandem repeats between the coding regions of E4.1 and RH5 genes. In summary, the presented pathological and molecular characterization of this case suggests that further research into the worldwide molecular epidemiology and disease burden of BAdV-7 is warranted.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Molecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in Germany

Jesse

Ciurkiewicz

Siesenop³

et al. 2022

Virol J

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“…DNA sequencing was performed with a deep sequencing protocol using Novaseq 6000 (Illumina). De novo assembly of the resultant reads and determination of 5' and 3' terminal sequences were performed as described previously [20]. The sequences were aligned using a Muscle program available in MEGA X (https://www.megasoftware.net/) with reference adenovirus strains retrieved from GenBank.…”

Section: Complete Genome Sequencing and Phylogenetic Analysismentioning

confidence: 99%

First Isolation, Molecular Characterization, and Serological Survey of Bovine Adenovirus Type 2 in Japan

Kubota

Orihara

Kumagai

et al. 2021

Preprint

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Bovine adenovirus type 2 (BAdV-2), a member of the Mastadenovirus genus of the Adenoviridae family, is involved in mild respiratory disease and is rarely isolated from cattle in the world. Here, we isolated BAdV-2 for the first time in Japan, from the feces of cattle with severe respiratory symptoms. The isolate, designated strain KY19-1, was identified morphologically and genetically. Electron microscopic observation of infected cells showed intranuclear, non-enveloped virus particles, approximately 70 nm in diameter and with typical adenoviral morphology. Whole-genome sequencing revealed that the viral genome is 33,175 bp long, with 105 bp inverted terminal repeats and encoding 32 predicted genes. KY19-1 has a similar genomic organization to the prototype BAdV-2 strain No. 19 with overall 99.1% nucleotide identity. Notable differences were found in the E3 region of the genome, which may affect various biological properties, including pathogenicity. The seroepidemiology of BAdV-2 was also investigated, using a virus-neutralization test, and 92.8 % of the Japanese cattle (n=1,325) were seropositive, with the positivity rate increased by age. Further experimental infections with KY19-1 may elucidate the pathogenic properties of BAdV-2 and the importance of this virus as a causal agent of bovine respiratory disease.

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“…A 29,941 bp contig was generated with an average base coverage depth of 217. To account for short nucleotide stretches lacking at both ends of the genome, we performed 5′ and 3′ adapter ligation, followed by PCR with specific primers, and Sanger sequencing was performed [ 6 ]. The full-length TS-GT genome sequence of 30,052 nucleotides, with 33.58% GC content, was obtained and deposited in DDBJ under the accession number LC606503.1.…”

mentioning

confidence: 99%

“…Sci. 84(8): 1118-1120, 2022 doi: 10.1292/jvms.22-0198 both ends of the genome, we performed 5′ and 3′ adapter ligation, followed by PCR with specific primers, and Sanger sequencing was performed [6]. The full-length TS-GT genome sequence of 30,052 nucleotides, with 33.58% GC content, was obtained and deposited in DDBJ under the accession number LC606503.1.…”

mentioning

confidence: 99%

Whole-genome sequencing of live attenuated bovine adenovirus type 7 vaccine strain TS-GT suggests biomarkers for virulence attenuation

Kumagai

Kajikawa

Miyazaki

et al. 2022

The Journal of Veterinary Medical Science

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Bovine adenovirus type 7 (BAdV-7) is one of the most important respiratory and enteric pathogens in the cattle industry. Although live attenuated vaccines are used to control the virus in Japan, limited information is available on the genomic regions that determine viral pathogenicity. We determined the complete genome sequence of the attenuated BAdV-7 strain TS-GT. The genome is 30,052 bp long and contains 45-bp inverted terminal repeats and 30 predicted genes. A genome sequence comparison showed that 99.9% of the TS-GT genome is identical to the prototypic and pathogenic BAdV-7 strain Fukuroi; however, the TS-GT genome contains a novel mutation and four indels. We describe here potential relationships between these genomic changes and the biological characteristics of BAdV-7.

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Complete Genome Sequence of Bovine Adenovirus Type 7 Strain Fukuroi, Isolated from a Cow with Respiratory Disease

Abstract: We determined the complete genome sequence of the bovine adenovirus type 7 prototype strain Fukuroi using next-generation sequencing technology. We found that the viral genome is 30,034 bp long and has the shortest inverted terminal repeats among known adenoviruses.

Cited by 6 publications

References 7 publications

Molecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in Germany

Molecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in Germany

First Isolation, Molecular Characterization, and Serological Survey of Bovine Adenovirus Type 2 in Japan

Whole-genome sequencing of live attenuated bovine adenovirus type 7 vaccine strain TS-GT suggests biomarkers for virulence attenuation

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