337 nm Matrix-assisted laser desorption/ionization of single aerosol particles

He, Lin; Murray, Kermit K.

doi:10.1002/(sici)1096-9888(199909)34:9<909::aid-jms849>3.0.co;2-a

Cited by 31 publications

(30 citation statements)

References 22 publications

(26 reference statements)

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“…Sampling standard particles composed of mixtures of matrix-assisted laser desorption ionization (MALDI) matrix and analyte provides a means to produce ions of a given m/z [3][4][5] and assess the instrument performance over a range of masses. The reflectron time-offlight (TOF) instrument was unable to detect ion signals from standard MALDI particles of angiotensin (MW ϭ 1297 Da) and ␣-cyclodextrin (MW ϭ 973 Da) but readily detected matrix ions.…”

Section: B Ioaerosol Mass Spectrometry (Bams) Has Recentlymentioning

confidence: 99%

See 1 more Smart Citation

Improved sensitivity and mass range in time-of-flight bioaerosol mass spectrometry using an electrostatic ion guide

Czerwieniec

Russell

Lebrilla

et al. 2005

J. Am. Soc. Mass Spectrom.

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Bioearosol mass spectrometry (BAMS) analyzes single particles in real time from ambient air, placing strict demands on instrument sensitivity. Modeling of the BAMS reflectron time of flight (TOF) with SIMION revealed design limitations associated with ion transmission and instrument sensitivity at higher masses. Design and implementation of a BAMS linear TOF with electrostatic ion guide and delayed extraction capabilities has greatly increased the sensitivity and mass range relative to the reflectron design. Initial experimental assessment of the new instrument design revealed improved sensitivity at high masses as illustrated when using standard particles of cytochrome C (m/z ϳ 12,000), from which the compound's monomer, dimer (m/z ϳ 24,000) and trimer (m/z ϳ 36,000) were readily detected. (J Am Soc Mass Spectrom 2005, 16, 1866 -1875

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Section: B Ioaerosol Mass Spectrometry (Bams) Has Recentlymentioning

confidence: 99%

“…Single particle MALDI experiments provide a suitable system to access the performance of the instrument [5][6][7]. Gramicidin S particles were generated from a 50/50 H 2 O/ethanol solution of 2,5-dihydroxybenzoic acid (DHB; 3 ϫ 10 Ϫ3 M) and gramicidin S at a 100:1 matrixto-analyte molar ratio.…”

Section: Particle Generation and Samplingmentioning

confidence: 99%

Improved sensitivity and mass range in time-of-flight bioaerosol mass spectrometry using an electrostatic ion guide

Czerwieniec

Russell

Lebrilla

et al. 2005

J. Am. Soc. Mass Spectrom.

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“…It has not been as well studied as bulk MALDI, but it does provide a unique opportunity for fundamental research into the MALDI process. The analysis of aerosol particles containing analyte and matrix was first reported in 1993 by Murray and coworkers [26], who would go on to publish numerous papers on the subject [27][28][29][30][31][32][33][34]. In 1996, however, Mansoori et al were the first to analyze such particles individually in a single particle TOFMS [35].…”

mentioning

confidence: 99%

Parameters contributing to efficient ion generation in aerosol MALDI mass spectrometry

McJimpsey

Jackson

Lebrilla

et al. 2008

J. Am. Soc. Mass Spectrom.

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The Bioaerosol Mass Spectrometry (BAMS) system was developed for the real-time detection and identification of biological aerosols using laser desorption ionization. Greater differentiation of particle types is desired; consequently MALDI techniques are being investigated. The small sample size (ϳ1 m 3 ), lack of substrate, and ability to simultaneously monitor both positive and negative ions provide a unique opportunity to gain new insight into the MALDI process. Several parameters known to influence MALDI molecular ion yield and formation are investigated here in the single particle phase. A comparative study of five matrices (2,6-dihydroxyacetophenone, 2,5-dihydroxybenzoic acid, ␣-cyano-4-hydroxycinnamic acid, ferulic ␣ acid, and sinapinic acid) with a single analyte (angiotensin I) is presented and reveals effects of matrix selection, matrix-to-analyte molar ratio, and aerosol particle diameter. The strongest analyte ion signal is found at a matrix-to-analyte molar ratio of 100:1. At this ratio, the matrices yielding the least and greatest analyte molecular ion formation are ferulic acid and ␣-cyano-␣ 4-hydroxycinnamic acid, respectively. Additionally, a significant positive correlation is found between aerodynamic particle diameter and analyte molecular ion yield for all matrices. SEM imaging of select aerosol particle types reveals interesting surface morphology and structure.

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“…Baseline resolution was observed and a resolution of 5000 (measured at FWHM) was calculated for the parent ions. This observed resolution is approximately an order of magnitude greater than that reported for timeof-flight-based aerosol MALDI [12,16,17,24]. In those instruments, aerosol beam width and methods of sample preparation have been suggested as limiting resolutions [13].…”

Section: Resultsmentioning

confidence: 99%

“…With their technique, they generated mass spectra of individual bacterial spores [12,13]. Other groups have also demonstrated MALDI on a single-particle level by either on-line coating of the particles [14,15] or premixing the matrix and analyte [16,17].…”

Section: Introductionmentioning

confidence: 99%

Aerosol MALDI of peptides and proteins in an ion trap mass spectrometer: Trapping, resolution and signal-to-noise

Harris¹,

Reilly²,

Whitten³

2006

International Journal of Mass Spectrometry

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337 nm Matrix-assisted laser desorption/ionization of single aerosol particles

Cited by 31 publications

References 22 publications

Improved sensitivity and mass range in time-of-flight bioaerosol mass spectrometry using an electrostatic ion guide

Improved sensitivity and mass range in time-of-flight bioaerosol mass spectrometry using an electrostatic ion guide

Parameters contributing to efficient ion generation in aerosol MALDI mass spectrometry

Aerosol MALDI of peptides and proteins in an ion trap mass spectrometer: Trapping, resolution and signal-to-noise

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