Downregulation of the E2 Subunit of 2-Oxoglutarate Dehydrogenase Modulates Plant Growth by Impacting Carbon–Nitrogen Metabolism in<i>Arabidopsis thaliana</i>

Condori-Apfata, Jorge A.; Batista‐Silva, Willian; Medeiros, David B.; Vargas, Jonas Rafael; Valente, Luiz Mário Lopes; Pérez-Díaz, Jorge Luis; Fernie, Alisdair R.; Araújo, Wagner L.; Nunes‐Nesi, Adriano

doi:10.1093/pcp/pcab036

Cited by 10 publications

(6 citation statements)

References 83 publications

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“…Interestingly, the functional lack of expression of E 2 subunit isoforms enhanced plant growth, decreased dark respiration, and reduced carbohydrate metabolism without altering the photosynthetic rate. [ 56 ] Reduction of the ODC activity via T‐DNA insertional knockout resulted in plants that exhibited a reduction of leaf total amino acids and minor changes in the levels of TCA cycle intermediates in Arabidopsis , suggesting the ODC plays a mediating role in carbon–nitrogen metabolism. [ 56 ] Similarly reducing tissue 2‐OGDH complex activity via inhibition of ODC by specific chemical inhibitors could limit respiration, as well as alter C and N metabolism demonstrating that this enzyme has an important function in nitrogen assimilation in potato tubers.…”

Section: The Organization Of the Plant Tca Cycle And Its Metabolic Co...mentioning

confidence: 99%

“…[ 56 ] Reduction of the ODC activity via T‐DNA insertional knockout resulted in plants that exhibited a reduction of leaf total amino acids and minor changes in the levels of TCA cycle intermediates in Arabidopsis , suggesting the ODC plays a mediating role in carbon–nitrogen metabolism. [ 56 ] Similarly reducing tissue 2‐OGDH complex activity via inhibition of ODC by specific chemical inhibitors could limit respiration, as well as alter C and N metabolism demonstrating that this enzyme has an important function in nitrogen assimilation in potato tubers. [ 57 ] It was, moreover, suggested that the level of transcripts of the ODC genes decreased in the light or upon exposure to red light in the leaves of maize ( Zea mays L .…”

Section: The Organization Of the Plant Tca Cycle And Its Metabolic Co...mentioning

confidence: 99%

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The Role of TCA Cycle Enzymes in Plants

Zhang

Fernie

2023

Advanced Biology

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As one of the iconic pathways in plant metabolism, the tricarboxylic acid (TCA) cycle is commonly thought to not only be responsible for the oxidization of respiratory substrate to drive ATP synthesis but also provide carbon skeletons to anabolic processes and contribute to carbon–nitrogen interaction and biotic stress responses. The functions of the TCA cycle enzymes are characterized by a saturation transgenesis approach, whereby the constituent expression of proteins is knocked out or reduced in order to investigate their function in vivo. The alteration of TCA cycle enzyme expression results in changed plant growth and photosynthesis under controlled conditions. Moreover, improvements in plant performance and postharvest properties are reported by overexpression of either endogenous forms or heterologous genes of a number of the enzymes. Given the importance of the TCA cycle in plant metabolism regulation, here, the function of each enzyme and its roles in different tissues are discussed. This article additionally highlights the recent finding that the plant TCA cycle, like that of mammals and microbes, dynamically assembles functional substrate channels or metabolons and discusses the implications of this finding to the current understanding of the metabolic regulation of the plant TCA cycle.

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Section: The Organization Of the Plant Tca Cycle And Its Metabolic Co...mentioning

confidence: 99%

Section: The Organization Of the Plant Tca Cycle And Its Metabolic Co...mentioning

confidence: 99%

The Role of TCA Cycle Enzymes in Plants

Zhang

Fernie

2023

Advanced Biology

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“…To further determine whether OGDH functions as part of KGDH to control floral transition, we examined loss-of-function mutants of E2 subunits. The Arabidopsis E2 subunit is encoded by two genes ( 30 ), E2a ( At5G55070 ) and E2b ( At4G26910 ), with very similar protein sequences (figs. S3A and S4, A and C).…”

Section: Kgdh Controls α-Kg Levels and Regulates Growth And Developme...mentioning

confidence: 99%

“…S3D). An e2a allele with a T-DNA insertion in its 3′ untranslated region was recently found to flower slightly later ( 30 ). We failed to obtain an e2a e2b double mutant through genetic crossing, likely due to an essential function of E2/KGDH in cellular respiration.…”

Section: Kgdh Controls α-Kg Levels and Regulates Growth And Developme...mentioning

confidence: 99%

Control of histone demethylation by nuclear-localized α-ketoglutarate dehydrogenase

Huang

Luo

et al. 2023

Science

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Methylations on nucleosomal histones play fundamental roles in regulating eukaryotic transcription. Jumonji C domain–containing histone demethylases (JMJs) dynamically control the level of histone methylations. However, how JMJ activity is generally regulated is unknown. We found that the tricarboxylic acid cycle–associated enzyme α-ketoglutarate (α-KG) dehydrogenase (KGDH) entered the nucleus, where it interacted with various JMJs to regulate α-KG–dependent histone demethylations by JMJs, and thus controlled genome-wide gene expression in plants. We show that nuclear targeting is regulated by environmental signals and that KGDH is enriched at thousands of loci in Arabidopsis thaliana . Chromatin-bound KGDH catalyzes α-KG decarboxylation and thus may limit its local availability to KGDH-coupled JMJs, inhibiting histone demethylation. Thus, our results uncover a regulatory mechanism for histone demethylations by JMJs.

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“…The TCA cycle modulates not only the flux from 2-OG to amino acid metabolism but also the flux through 2ODDs that connects the TCA cycle with glucosinolate, flavonoid, and alkaloid formation, as well as with different reactions of amino acid and GA biosynthesis (reviewed by Araújo et al, 2014 ). The direct role of the TCA cycle in these reactions was shown through the inhibition of the mitochondrial 2-OG dehydrogenase complex ( Araújo et al, 2012a , 2012 b ; Condori-Apfata et al, 2021 ). In the mitochondrial matrix, citrate synthase controls not only the production of citrate that leads to the formation of 2-OG entering the 2ODD reactions but also the formation of products of metabolic pathways initiated by 2ODDs, e.g.…”

Section: Plant Mitochondria Are the Drivers Of 2odd Reactionsmentioning

confidence: 99%

Mitochondria in photosynthetic cells: Coordinating redox control and energy balance

Igamberdiev

Bykova

2022

Plant Physiology

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In photosynthetic tissues in the light, the function of energy production is associated primarily with chloroplasts, while mitochondrial metabolism adjusts to balance ATP supply, regulate the reduction level of pyridine nucleotides, and optimize major metabolic fluxes. The tricarboxylic acid cycle in the light transforms into a non-cyclic open structure (hemicycle) maintained primarily by influx of malate and export of citrate to the cytosol. The exchange of malate and citrate forms the basis of feeding redox energy from the chloroplast into the cytosolic pathways. This supports the level of NADPH in different compartments, contributes to the biosynthesis of amino acids, and drives secondary metabolism via the supply of substrates for 2-oxoglutarate dioxygenase and for cytochrome P450-catalyzed monooxygenase reactions. This results in the maintenance of redox and energy balance in photosynthetic plant cells and in the formation of numerous bioactive compounds specific for any particular plant species. The non-coupled mitochondrial respiration operates in coordination with the malate and citrate valves and supports intensive fluxes of respiration and photorespiration. The metabolic system of plants has features associated with remarkable metabolic plasticity of mitochondria that permit the use of energy accumulated in photosynthesis in a way that all anabolic and catabolic pathways become optimized and coordinated.

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Downregulation of the E2 Subunit of 2-Oxoglutarate Dehydrogenase Modulates Plant Growth by Impacting Carbon–Nitrogen Metabolism inArabidopsis thaliana

Cited by 10 publications

References 83 publications

The Role of TCA Cycle Enzymes in Plants

The Role of TCA Cycle Enzymes in Plants

Control of histone demethylation by nuclear-localized α-ketoglutarate dehydrogenase

Mitochondria in photosynthetic cells: Coordinating redox control and energy balance

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