Analyses of transcriptomics and metabolomics reveal pathway of vacuolar Sur7 contributed to biocontrol potential of entomopathogenic Beauveria bassiana

Zhang, Longbin; Qiu, Tingting; Guan, Yi; Huang, Zhihong; Ye, Xiuyun

doi:10.1016/j.jip.2021.107564

Cited by 5 publications

(3 citation statements)

References 54 publications

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“…In B . bassiana , the deletion of the Sur7 gene, related to stress tolerance, fungal development, and virulence, led to a reduction in mannitol production [ 53 ]. Consequently, the authors found that susceptibility to oxidative stress increased and sporulation reduced, demonstrating the molecule’s importance in fungal pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

Fungal consortium of two Beauveria bassiana strains increases their virulence, growth, and resistance to stress: A metabolomic approach

et al. 2022

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The use of two or more microorganisms in a microbial consortium has been increasingly applied in the biological control of diseases and pests. Beauveria bassiana is one of the most widely studied fungal species in biological control, yet little is known about its role in fungal consortiums. In a previous study, our group found that a consortium formed by two strains of B. bassiana had significantly greater biocontrol potential against the polyphagous caterpillars Duponchelia fovealis (Lepidoptera: Crambidae) than either strain on its own. In this study, we use GC-MS and LC-MS/MS to evaluate and discuss the metabolomics of the consortium. A total of 21 consortium biomarkers were identified, corresponding to 14 detected by LC-MS/MS and seven by GC-MS. Antioxidant and anti-inflammatory mechanisms are the main properties of the metabolites produced by the consortium. These metabolites can depress the insect’s immune system, increasing its vulnerability and, hence, the fungal virulence of the consortium. In light of these results, we propose an action model of insect mortality due to the metabolites secreted by the consortium. The model includes the inhibition of defense mechanisms such as pro-inflammatory interleukin secretion, cell migration, cell aggregation, Dif, Dorsal and Relish gene transcription, and JAK/STAT and JNK signaling pathways. It also promotes the cleaning of oxidative molecules, like ROS, NOS, and H2O2, and the induction of virulence factors.

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Section: Discussionmentioning

confidence: 99%

Fungal consortium of two Beauveria bassiana strains increases their virulence, growth, and resistance to stress: A metabolomic approach

et al. 2022

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“…After Cas9-encoding gene is transformed into host strains using common delivery strategies, the cas9 gene is generally randomly integrated into the genome, which may affect the host genome structure, leading to unexpected phenotypes, such as growth delay, further rearrangements or off-target mutations, and toxic effects on the host . Therefore, it is important to ensure that Cas9 protein has no adverse effects on filamentous fungi by testing the biological properties, such as development, stress resistance and virulence of Cas9-expressing strains. − …”

Section: The Functional Components Of the Crispr/cas9 Systemmentioning

confidence: 99%

Application of CRISPR in Filamentous Fungi and Macrofungi: From Component Function to Development Potentiality

Zhao

2023

ACS Synth. Biol.

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Fungi, particularly filamentous fungi and macrofungi, have a very powerful ability to produce secondary metabolites and can serve as excellent chassis cells for the production of enzymes or natural products of great value in synthetic biology. Thus, it is imperative to establish simple, reliable, and efficient techniques for their genetic modification. However, the heterokaryosis of some fungi and the dominance of nonhomologous end-joining (NHEJ) repair mechanisms in vivo have been greatly affecting the efficiency of fungal gene editing. In recent years, the CRISPR/Cas9 system has been applied as a widely used gene editing technology in life science research and has also played an important role in the genetic modification of filamentous and macrofungi. The various functional components (cas9, sgRNA, promoter, and screening marker) of the CRISPR/Cas9 system and its development, as well as the difficulties and potential of the CRISPR/Cas9 system in filamentous fungus and macrofungi, are the main topics of this paper.

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“…Studies on genomics (e.g., [27,28]), transcriptomics (e.g., [21,29]), metabolomics (e.g., [30,31]), and secretomics (e.g., [32,33]) of B. bassiana have been reported one after another, which are conducive to reproducing more details of entomopathogenic fungal infection of insects. Considering the dynamics and complexity of this process, many researchers have tried to use time-series data to monitor their changes, and the time-series analysis has been proven to have obvious advantages for transcriptomic data [34].…”

Section: Introductionmentioning

confidence: 99%

Time‐series gene expression patterns and their characteristics of Beauveria bassiana in the process of infecting pest insects

Zhang

Yao³

et al. 2022

J Basic Microbiol

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Beauveria bassiana has been widely used as an important biological control fungus for agricultural and forest pests, and clarifying the interaction mechanism between B. bassiana and its host will help to better exert the efficacy of the mycoinsecticide. Here, we proposed a novel pattern analysis (PA) method for analyzing time-series data and applied it to a transcriptomic data set of B. bassiana infecting Galleria mellonella. We screened out 14 patterns including 868 genes, which had some characteristics that were not inferior to differentially expressed genes (DEGs). Compared with the previous analysis of this data set, we had three novel discoveries during B. bassiana infection, including overall downregulation of gene expression, the more critical first 24 h, and enrichment of regulatory functions of downregulated genes. Our new PA method promises to be an important complement to DEGs

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Analyses of transcriptomics and metabolomics reveal pathway of vacuolar Sur7 contributed to biocontrol potential of entomopathogenic Beauveria bassiana

Cited by 5 publications

References 54 publications

Fungal consortium of two Beauveria bassiana strains increases their virulence, growth, and resistance to stress: A metabolomic approach

Fungal consortium of two Beauveria bassiana strains increases their virulence, growth, and resistance to stress: A metabolomic approach

Application of CRISPR in Filamentous Fungi and Macrofungi: From Component Function to Development Potentiality

Time‐series gene expression patterns and their characteristics of Beauveria bassiana in the process of infecting pest insects

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