Three-dimensional tissue fabrication system by co-culture of microalgae and animal cells for production of thicker and healthy cultured food

Haraguchi, Yuji

doi:10.1007/s10529-021-03106-0

Cited by 16 publications

(10 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, reducing the cost of albumin, growth factors and basal media (e.g., through the use of plant or algal hydrolysates) will be essential 26,27 . Additionally, co-culture of meat-relevant cells with nutrient-or growth factor-producing cells could offer valuable cost-saving opportunities 28 . When considering Beefy-9, it is clear that recombinant proteins are the main drivers of cost.…”

Section: Discussionmentioning

confidence: 99%

Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat

et al. 2021

Preprint

View full text Add to dashboard Cite

Cell-cultured meat offers the potential for a more sustainable, ethical, resilient, and healthy food system. However, research and development has been hindered by the lack of suitable serum-free media that enable the robust expansion of relevant cells (e.g., muscle satellite cells) over multiple passages. Recently, a low-cost serum-free media (B8) was described for induced pluripotent stem cells. Here, we adapt this media for bovine satellite cells and show that the addition of a single component, recombinant albumin, renders B8 suitable for the long-term expansion of cells without sacrificing myogenicity. We show that this new media (Beefy-9) maintains robust cell growth over the entire culture period tested (seven passages) with an average growth rate of 39 hours per population doubling). Along with demonstrated efficacy for bovine cells, this work provides a promising starting-point for developing serum-free media for cultures from other meat-relevant species. Ultimately, this work offers a promising foundation for escaping the reliance on serum in cultured meat research, thereby accelerating the field.

show abstract

Section: Discussionmentioning

confidence: 99%

Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Although substantial progress has been achieved in recent years by both for‐profit companies and academic groups, CM remains a relatively new area of investigation. As might be expected for a nascent field that draws heavily on knowledge gained from previous work aimed at a distinct set of problems, review, and perspective papers about CM (at least 50 by our estimate) [ 10–14,16,18,23,28,37,44,64–109 ] currently outnumber original research papers where CM production is the primary intended application (at least 24) [ 15,59,110–131 ] or CM‐related patents (at least 24). [ 132–155 ] These estimates do not include LCA or TEA studies, consumer acceptance studies, or purely computational analyses.…”

Section: Cultivated Meatmentioning

confidence: 93%

Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges

Bomkamp¹,

Skaalure²,

Fernando³

et al. 2021

Advanced Science

151

View full text Add to dashboard Cite

Cultivating meat from stem cells rather than by raising animals is a promising solution to concerns about the negative externalities of meat production. For cultivated meat to fully mimic conventional meat's organoleptic and nutritional properties, innovations in scaffolding technology are required. Many scaffolding technologies are already developed for use in biomedical tissue engineering. However, cultivated meat production comes with a unique set of constraints related to the scale and cost of production as well as the necessary attributes of the final product, such as texture and food safety. This review discusses the properties of vertebrate skeletal muscle that will need to be replicated in a successful product and the current state of scaffolding innovation within the cultivated meat industry, highlighting promising scaffold materials and techniques that can be applied to cultivated meat development. Recommendations are provided for future research into scaffolds capable of supporting the growth of high-quality meat while minimizing production costs. Although the development of appropriate scaffolds for cultivated meat is challenging, it is also tractable and provides novel opportunities to customize meat properties.

show abstract

“…In order to produce healthier and thicker cultured food, animal cells can be co-cultivated with algae for 3D tissue fabrication. Thus, Haraguchi and Shimizu co-cultivated C2C12 cells and photosynthetic autotrophic microalgae Chlorella vulgaris ( C. vulgaris ) [ 69 ].…”

Section: Major Cell Types With Potential For Use In Cultivated Meat (...mentioning

confidence: 99%

“…The same waste management principles, with the additional possibility of recycling media, could be considered when it comes to the unutilized nutrients, including glucose, amino acids, carbohydrates and proteins [ 144 , 318 ], and generated metabolites such as ammonia. Haraguchi et al [ 69 , 319 ] have proposed an interesting in vitro “symbiotic recycling system” composed of mammalian cells and algae, resulting in significantly reduced glucose consumption by the C2C12 cells and rat cardiac cells and therefore lower metabolic production of lactate and ammonia, with simultaneous generation of a larger thickness tissue [ 69 ]. The downstream process should be created to enable the recycling of the valuable components and removal of the unwanted ones.…”

Section: Bioprocess Conditions For Production Of CMmentioning

confidence: 99%

Bioengineering Outlook on Cultivated Meat Production

et al. 2022

View full text Add to dashboard Cite

Cultured meat (also referred to as cultivated meat or cell-based meat)—CM—is fabricated through the process of cellular agriculture (CA), which entails application of bioengineering, i.e., tissue engineering (TE) principles to the production of food. The main TE principles include usage of cells, grown in a controlled environment provided by bioreactors and cultivation media supplemented with growth factors and other needed nutrients and signaling molecules, and seeded onto the immobilization elements—microcarriers and scaffolds that provide the adhesion surfaces necessary for anchor-dependent cells and offer 3D organization for multiple cell types. Theoretically, many solutions from regenerative medicine and biomedical engineering can be applied in CM-TE, i.e., CA. However, in practice, there are a number of specificities regarding fabrication of a CM product that needs to fulfill not only the majority of functional criteria of muscle and fat TE, but also has to possess the sensory and nutritional qualities of a traditional food component, i.e., the meat it aims to replace. This is the reason that bioengineering aimed at CM production needs to be regarded as a specific scientific discipline of a multidisciplinary nature, integrating principles from biomedical engineering as well as from food manufacturing, design and development, i.e., food engineering. An important requirement is also the need to use as little as possible of animal-derived components in the whole CM bioprocess. In this review, we aim to present the current knowledge on different bioengineering aspects, pertinent to different current scientific disciplines but all relevant for CM engineering, relevant for muscle TE, including different cell sources, bioreactor types, media requirements, bioprocess monitoring and kinetics and their modifications for use in CA, all in view of their potential for efficient CM bioprocess scale-up. We believe such a review will offer a good overview of different bioengineering strategies for CM production and will be useful to a range of interested stakeholders, from students just entering the CA field to experienced researchers looking for the latest innovations in the field.

show abstract

Three-dimensional tissue fabrication system by co-culture of microalgae and animal cells for production of thicker and healthy cultured food

Cited by 16 publications

References 40 publications

Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat

Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat

Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges

Bioengineering Outlook on Cultivated Meat Production

Contact Info

Product

Resources

About