Enrichment of Placental Trophoblast Cells from Clinical Cervical Samples Using Differences in Surface Adhesion on an Inclined Plane

Bailey‐Hytholt, Christina M.; Sayeed, Sumaiya; Shukla, Anita; Tripathi, Anubhav

doi:10.1007/s10439-021-02742-x

Cited by 6 publications

(4 citation statements)

References 30 publications

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“…They used gravity to make the cells move along the inclined plane, collecting trophoblasts in heterogeneous cervical cell populations, and identifying JEG-3 trophoblasts by HLA-G antibody staining and fluorescence imaging. It was confirmed that the purity of trophoblast in clinical cervical samples can be increased by 396 ± 52% (117). The design of the enrichment device can be used by non-professionals with low cost and high speed and can be directly integrated into the automatic cell picker instrument on the surface of the slide.…”

Section: Application Of Hla Class Ib In Assisted Reproductionmentioning

confidence: 83%

Advances in the study of HLA class Ib in maternal-fetal immune tolerance

et al. 2022

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The HLA class Ib molecule is an alloantigen that causes transplant rejection on behalf of individual human and plays an important role in maternal-fetal immune tolerance. Early studies on HLA class Ib focused on the mechanism of HLA-G-induced immune escape, but in recent years, studies on the mechanism of HLA-G have deepened and gradually explored the mechanism of HLA-E and HLA-F, which are also HLA class Ib molecules. In the maternal-fetal interface, trophoblast cells express HLA class Ib molecules to protect the fetus from maternal immune cells by binding to inhibitory receptors of decidual immune cells (DICs) and shifting Th1/Th2 balance toward Th2 bias. Further studies on the molecular mechanism of HLA class Ib molecules provide a reference for its application in the field of clinical assisted reproduction.

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Section: Application Of Hla Class Ib In Assisted Reproductionmentioning

confidence: 83%

Advances in the study of HLA class Ib in maternal-fetal immune tolerance

et al. 2022

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“…Moreover, NIPS false-positive and false-negative test results have been highlighted by the US FDA in a recent safety communication [ 29 ]. There have been several recent studies demonstrating successful cell-based NIPD using trophoblasts from maternal blood [ 30 , 31 , 32 , 33 ] or from the cervix [ 34 , 35 , 36 ], which are present in the circulation at higher numbers, are easier to isolate, and are free of maternal DNA contamination; however, these studies do not totally avoid CPM [ 30 , 31 ]. Our results serve to highlight the advantage of FNRBCs over trophoblasts, namely, that FNRBCs represent the true fetal genotype, originating from the yolk sac and then from fetal liver at later gestations [ 37 ], and can be found within first-trimester placental villi, where they undergo terminal maturation processes [ 38 ], whereas trophoblasts are derived from the placenta.…”

Section: Discussionmentioning

confidence: 99%

Whole-Chromosome Karyotyping of Fetal Nucleated Red Blood Cells Using the Ion Proton Sequencing Platform

Barrett

Huang

Aung

et al. 2022

Genes

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The current gold standard for the definitive diagnosis of fetal aneuploidy uses either chorionic villus sampling (CVS) or amniocentesis, both of which are which are invasive procedures carrying a procedure-related risk of miscarriage of up to 0.1%–0.2%. Non-invasive prenatal diagnosis using fetal nucleated red blood cells (FNRBCs) isolated from maternal peripheral venous blood would remove this risk of miscarriage since these cells can be isolated from the mother’s blood. We aimed to detect whole-chromosome aneuploidies from single nucleated fetal red blood cells using whole-genome amplification followed by massively parallel sequencing performed on a semiconductor sequencing platform. Twenty-six single cells were picked from the placental villi of twelve patients thought to have a normal fetal genotype and who were undergoing elective first-trimester surgical termination of pregnancy. Following karyotyping, it was subsequently found that two of these cases were also abnormal (one trisomy 15 and one mosaic genotype). One single cell from chorionic villus samples for two patients carrying a fetus with trisomy 21 and two single cells from women carrying fetuses with T18 were also picked. Pooled libraries were sequenced on the Ion Proton and data were analysed using Ion Reporter software. We correctly classified fetal genotype in all 24 normal cells, as well as the 2 T21 cells, the 2 T18 cells, and the two T15 cells. The two cells picked from the fetus with a mosaic result by CVS were classified as unaffected, suggesting that this was a case of confined placental mosaicism. Fetal sex was correctly assigned in all cases. We demonstrated that semiconductor sequencing using commercially available software for data analysis can be achieved for the non-invasive prenatal diagnosis of whole-chromosome aneuploidy with 100% accuracy.

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“…[457] Similar principles have also been applied to the sorting and analysis of rare bacterial cells within blood and urine, as well as fetal cells in prenatal diagnostics. [387,388,414,458,459] Rare cell biosensors illustrate some of the more creative nanotechnology approaches that can overcome limitations of many traditional instruments and diagnostic techniques.…”

Section: Clinical Applications Of Nanomaterials In Diagnosticsmentioning

confidence: 99%

Advances in Biosensors and Diagnostic Technologies Using Nanostructures and Nanomaterials

Welch

Powell

Clevinger

et al. 2021

Adv Funct Materials

Self Cite

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Nanoscale materials have unique properties that make them especially useful for biomedical diagnostic applications. Recent developments in nanoengineering have resulted in increasing use of nanostructures in biosensors. Various types of 0D, 1D, 2D, and 3D nanostructures have been used to improve biosensor sensitivity, selectivity, limit of detection, and time to result, among other metrics. These nanostructures have been integrated into electrochemical, optical, and other biosensors for this purpose. Here, the most recent advances in the use of nanostructured materials in biosensors are described. This includes a discussion of nanoparticles, nanorods, nanofibers, nanopillars, nanowires, nanosheets, indented nanopatterns (nanoholes and nanoslits), nanogaps, nanochannels, nanopores, nanofunctionalized surfaces, and complex hierarchical structures and their unique advantages and applications in biosensors. Clinical applications of these nanobiosensors are also highlighted along with a discussion of the future directions for these materials in diagnostics.

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Enrichment of Placental Trophoblast Cells from Clinical Cervical Samples Using Differences in Surface Adhesion on an Inclined Plane

Cited by 6 publications

References 30 publications

Advances in the study of HLA class Ib in maternal-fetal immune tolerance

Advances in the study of HLA class Ib in maternal-fetal immune tolerance

Whole-Chromosome Karyotyping of Fetal Nucleated Red Blood Cells Using the Ion Proton Sequencing Platform

Advances in Biosensors and Diagnostic Technologies Using Nanostructures and Nanomaterials

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