Direct Tissue PCR and Genotyping for Species Identification in a Case of Laryngeal Blastomycosis

Fritsche, Thomas R.; Anderson, Jennifer L.; Bassi, Deepa; Hall, Matthew C.; Boyle, Timothy R.

doi:10.1177/0145561321991342

Cited by 2 publications

(4 citation statements)

References 18 publications

(25 reference statements)

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“…Rapid, PCR-based genotyping of Blastomyces samples is now possible and could allow clinicians to better predict which patients may require hospitalization and which patients should undergo additional testing for disseminated infection. 31 This study has several important limitations, including that a sample size of 201 may not be sufficient to detect more subtle differences in allele variation. We also targeted specific SNPs rather than performing whole genome sequencing; while economical, this method covers only a very small portion of the human genome.…”

Section: Discussionmentioning

confidence: 97%

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Clinical Presentation of Blastomycosis is Associated With Infecting Species, Not Host Genotype

Linz,

Frost,

Kitchner

et al. 2024

Clin Med Res

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To determine if host genetics may be a risk factor for severe blastomycosis.Design: A cohort of patients who had contracted blastomycosis underwent targeted SNP (single nucleotide polymorphism) genotyping. The genetics of these patients were compared to a set of age and gender-matched controls and between patients with severe versus mild to moderate blastomycosis. Setting: The Marshfield Clinic Health System in central and northern WisconsinParticipants: Patients with a diagnosis of blastomycosis prior to 2017 were contacted for enrollment in this study. A phone hotline was also set up to allow interested participants from outside the Marshfield Clinic Health System to request enrollment.Methods: SNP frequency was assessed for significant differences between the patient cohort and controls and between patients with severe versus mild to moderate blastomycosis. We also tested the effect of Blastomyces species identified in clinical isolates on disease symptoms and severity.Results: No significant differences were found in SNP frequency between cases and controls or between those with severe or mild to moderate blastomycosis. We did detect significant differences in symptom frequency and disease severity by Blastomyces species.Conclusions: Our study did not identify any genetic risk factors for blastomycosis. Instead, the species of Blastomyces causing the infection had a significant effect on disease severity.

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Section: Discussionmentioning

confidence: 97%

“…29 We also note that our dataset was predominantly male (58%). This is commonly observed in blastomycosis datasets, 30,31 but as females have a higher rate of mortality from blastomycosis, 30 may have skewed our results.…”

Section: Variable Categorymentioning

confidence: 87%

Clinical Presentation of Blastomycosis is Associated With Infecting Species, Not Host Genotype

Linz,

Frost,

Kitchner

et al. 2024

Clin Med Res

View full text Add to dashboard Cite

show abstract

“…More recent rapid diagnostic testing includes real-time PCR assays. 9 Standard treatment includes itraconazole for a duration of 6 to 12 months. 10 In most reported cases, patients can expect resolution of symptoms after completing treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Definitive diagnosis is made by isolation of blastomyces in cultures; however, this can take a few weeks. More recent rapid diagnostic testing includes real-time PCR assays . Standard treatment includes itraconazole for a duration of 6 to 12 months .…”

Section: Discussionmentioning

confidence: 99%