Dosimetry for Cell Irradiation using Orthovoltage (40-300 kV) X-Ray Facilities

Santos, M. Dos; Paget, Vincent; Trompier, F.; Gruel, Gaëtan; Milliat, Fabien

doi:10.3791/61645

Cited by 10 publications

(12 citation statements)

References 1 publication

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“…13 A possible increase in the amount of cell suspension per test tube would significantly increase the dose gradient due to the simultaneous increase in volume. 13,14 In these instances, it is almost unavoidable to irradiate cells in multi-well plates or multiple containers.…”

Section: Discussionmentioning

confidence: 99%

“…However, it should be noted that this is not suitable for many biological experiments and high‐throughput molecular epidemiological studies due to the elevated variance of the assay and maximum workload 13 . A possible increase in the amount of cell suspension per test tube would significantly increase the dose gradient due to the simultaneous increase in volume 13,14 . In these instances, it is almost unavoidable to irradiate cells in multi‐well plates or multiple containers.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, most X-ray cabinets are constructed as high-or full-protection devices. 13,14 Due to the modality of X-ray production, with electrons impinging on a slightly angled anode, some spatial inhomogeneity is expected within X-ray radiation beams, especially at short distances from the focal spot. This is mainly due to the anode heel effect.…”

Section: Introductionmentioning

confidence: 99%

“…In comparison to classical radionuclide sources, X‐ray cabinets show improvements in terms of radiation protection due to the fact that they are a nonpermanent radiation source that is easier to maintain and dispose of. Furthermore, most X‐ray cabinets are constructed as high‐ or full‐protection devices 13,14 …”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Radiation field and dose inhomogeneities using an X‐ray cabinet in radiation biology research

et al. 2021

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Purpose: X-ray cabinets are replacing 137 Cs/ 60 Co sources in radiation biology research due to advantages in size, handling, and radiation protection. However, because of their different physical properties, X-ray cabinets are more susceptible to experimental influences than conventional sources. The aim of this study was to examine the variations related to the experimental setups typically used to investigate biological radiation effects with X-ray cabinets. Materials and methods: A combined approach of physical dose measurements by thermoluminescence dosimetry and detection of biological effects by quantification of γH2AX and 53BP1 foci was used to analyze field inhomogeneity and evaluate the influence of the components of the experimental setup. Results: Irradiation was performed using an X-ray tube (195 kV,10 mA,0.5-mmthick copper filter, dose rate of 0.59 Gy/min). Thermoluminescence dosimetry revealed inhomogeneity and a dose decrease of up to 42.3% within the beam area (diameter 31.1 cm) compared to the dose at the center. This dose decrease was consistent with the observed decline in the number of radiation-induced foci by up to 55.9 %. Uniform dose distribution was measured after reducing the size of the radiation field (diameter 12.5 cm). However, when using 15-ml test tubes placed at different positions within this field, the dose decreased by up to 17% in comparison to the central position. Analysis of foci number revealed significant differences between the tubes for γH2AX (1 h) and 53BP1 (4 h) at different time points after irradiation. Neither removal of some tubes nor of the caps improved the dose decrease significantly. By contrast, when using 1.5-ml tubes, dose differences were less than 4%, and no significant differences in foci number were detected. Conclusion: X-ray cabinets are user-friendly irradiation units for investigating biological radiation effects. However, field inhomogeneities and experimental setup components considerably affect the delivered irradiation doses. For this reason, strict dosimetric monitoring of experimental irradiation setups is mandatory for reliable studies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Radiation field and dose inhomogeneities using an X‐ray cabinet in radiation biology research

et al. 2021

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“…Irradiations were performed at 80 kV, 24 mA with an additional filtration of 0.15 mm of copper to mimic the energy spectrum used in interventional radiology. The Half Value Layer (HVL) is 0.138 ± 0.006 mm of copper, measured by following the protocol described in Dos Santos et al ( Dos Santos et al, 2021a ) adapted from the AAPM TG61 protocol ( Ma et al, 2001 ).…”

Section: Methodsmentioning

confidence: 99%

Preclinical modeling of low energy X-rays radiological burn: Dosimetry study by monte carlo simulations and EPR spectroscopy

et al. 2022

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Interventional radiology has grown considerably over the last decades and become an essential tool for treatment or diagnosis. This technique is mostly beneficial and mastered but accidental overexposure can occur and lead to the appearance of deterministic effects. The lack of knowledge about the radiobiological consequences for the low-energy X-rays used for these practices makes the prognosis very uncertain for the different tissues. In order to improve the radiation protection of patients and better predict the risk of complications, we implemented a new preclinical mouse model to mimic radiological burn in interventional radiology and performed a complete characterization of the dose deposition. A new setup and collimator were designed to irradiate the hind legs of 15 mice at 30 Gy in air kerma at 80 kV. After irradiation, mice tibias were collected to evaluate bone dose by Electron Paramagnetic Resonance (EPR) spectroscopy measurements. Monte Carlo simulations with Geant4 were performed in simplified and voxelized phantoms to characterize the dose deposition in different tissues and evaluate the characteristics of secondary electrons (energy, path, momentum). 30 mice tibias were collected for EPR analysis. An average absorbed dose of 194.0 ± 27.0 Gy was measured in bone initially irradiated at 30 Gy in air kerma. A bone to air conversion factor of 6.5 ± 0.9 was determined. Inter sample and inter mice variability has been estimated to 13.9%. Monte Carlo simulations shown the heterogeneity of the dose deposition for these low X-rays energies and the dose enhancement in dense tissue. The specificities of the secondary electrons were studied and showed the influence of the tissue density on energies and paths. A good agreement between the experimental and calculated bone to air conversion factor was obtained. A new preclinical model allowing to perform radiological burn in interventional radiology-like conditions was implemented. For the development of new preclinical radiobiological model where the exact knowledge of the dose deposited in the different tissues is essential, the complementarity of Monte Carlo simulations and experimental measurements for the dosimetric characterization has proven to be a considerable asset.

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Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive events

Paget

Guipaud

François

et al. 2024

Methods in Cell Biology

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Dosimetry for Cell Irradiation using Orthovoltage (40-300 kV) X-Ray Facilities

Cited by 10 publications

References 1 publication

Radiation field and dose inhomogeneities using an X‐ray cabinet in radiation biology research

Radiation field and dose inhomogeneities using an X‐ray cabinet in radiation biology research

Preclinical modeling of low energy X-rays radiological burn: Dosimetry study by monte carlo simulations and EPR spectroscopy

Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive events

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