Lscβ and lscγ, two novel levansucrases of Pseudomonas syringae pv. actinidiae biovar 3, the causal agent of bacterial canker of kiwifruit, show different enzymatic properties

Luti, Simone; Campigli, Sara; Ranaldi, Francesco; Paoli, Paolo De; Pazzagli, Luigia; Marchi, Guido

doi:10.1016/j.ijbiomac.2021.02.189

Cited by 15 publications

(11 citation statements)

References 56 publications

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“…Some of these polysaccharides are important in the initial phase of infection as they mask and protect the microbial agent from the host’s defense mechanisms. Other polysaccharides produced by P. syringae lead to hydrolysis reactions, consequent fructose release, and ripening [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

A Chromatic Enzymatic Time-Temperature Integrator Device Based on the Degradation of Phenolic Compounds for the Real-Time Prediction of the Quality and Shelf Life of Cherries

Gaspar

Alves

Quelhas

et al. 2023

Foods

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The particular characteristics of cherries, such as color, firmness, and palate increase their demand, as does, among other things, their antioxidant properties that benefit human health. However, their high perishability leads to a reduced shelf life and consequently generates undesirable changes in the cherry flow chain. To ensure food quality and safety and prevent food waste, a smart device prototype is proposed. The concepts related to the formulation and design of the enzymatic-type chromatic time-temperature integrator (TTI) device used to monitor the real-time quality of cherries are described. The kinetic parameters for thermal inactivation of cultivar Santina cherries were determined based on the degradation of phenolic compounds that are substrates of the polyphenol oxidase enzyme, whose hydroxylation reaction of a monophenol to o-diphenol leads to the oxidation in o-quinone. The proposed device concept aims to help retailers and consumers decide upon selling and buying according to the remaining shelf life, thus promoting sustainability related to food processes.

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Section: Introductionmentioning

confidence: 99%

A Chromatic Enzymatic Time-Temperature Integrator Device Based on the Degradation of Phenolic Compounds for the Real-Time Prediction of the Quality and Shelf Life of Cherries

Gaspar

Alves

Quelhas

et al. 2023

Foods

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“…glycinea PG4180 (Hettwer et al ., 1998; Li and Ullrich, 2001; Srivastava et al ., 2012; Khandekar et al ., 2014) and more recently in Pseudomonas syringae pv. actinidiae ( Psa ) (Luti et al ., 2021). It has been ascertained that three copies of the levansucrase gene are present within the genome of these strains, two of which expressed differentially ( lscB and lscC ) whilst lscA is not expressed at all.…”

Section: Introductionmentioning

confidence: 99%

“…tomato DC3000 and T1 strains, in Pseudomonas savastanoi pv phaseolicola 1448A and in Pseudomonas syringae pv. actinidiae (O'Brien et al ., 2011; Luti et al ., 2021).…”

Section: Introductionmentioning

confidence: 99%

Complete genome assembly of the levan‐positive strain PVFi1 of Pseudomonas savastanoi pv. savastanoi isolated from olive knots in Central Italy

Turco

Drais

Rossini

et al. 2022

Environ Microbiol Rep

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Pseudomonas savastanoi pv. savastanoi, the causal agent of olive knot disease, is a fluorescent Gramnegative bacterium classified, according to the specific LOPAT profile, as Ib. However, during the 90s, a number of atypical non-fluorescent levan-positive strains of Pseudomonas savastanoi pv. savastanoi have been unexpectedly isolated from olive knots in Central Italy. Since its first report, several studies were conducted on this species variant, but its genome sequence has never been reported. The complete genome sequence and two additional plasmids of PVFi1, a representative strain, were here obtained using a hybrid sequencing approach with both Oxford Nanopore Technology and Illumina sequencing. A thorough genomic analysis unravelled several genetic features of this peculiar strain, showing a transposase insertion downstream a fragmented copy of the levansucrase gene. The same features were previously reported on levan-negative Pseudomonas savastanoi pv. savastanoi strains. In addition, a second copy of the levansucrase gene fully equipped for a gene expression and comparable to the levan-positive Pseudomonas savastanoi pv. glycinea, may explain the levan-positive test. This result provides a solid genetic demonstration that the bacterial species Pseudomonas savastanoi contains either levan-positive or levan-negative strains, providing insights for an update of the related LOPAT classification.

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“…Kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiae ( Psa ) is an epidemic disease during the production of kiwifruit (Vanneste, 2017 ; Froud et al, 2018 ), which often results in sudden and severe economic losses and limitations in kiwifruit cultivation worldwide (Luti et al, 2021 ). People have explored various management strategies for controlling this disease (Bai et al, 2022 ), including but not limited to the application of antibiotics or copper and the breeding of resistant varieties (Wicaksono et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Response of Soil Microbial Community Structure Mediated by Sulfur-Induced Resistance Against Kiwifruit Bacterial Canker

et al. 2022

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Kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) is a major threat to kiwifruit worldwide, and effective control measures are still lacking. Sulfur, as a mineral, has been proved to improve plants' resistance to pathogens. It is of great significance to study the effect of sulfur on rhizosphere microorganisms in kiwifruit planting areas infected by Psa for controlling kiwifruit canker. In this study, the sulfur powder and organic fertilizer were mixed as base fertilizer to treat the soil in the area where kiwifruit bacterial canker occurs. We investigated the incidence of kiwifruit bacterial canker in 2018 and 2019 after sulfur application and the changes in microbial characteristics and community composition structure in the kiwifruit rhizosphere by using the plate-counting method and high-throughput sequencing technology. Fertilization treatments of kiwifruit roots with sulfur and organic fertilizer reduced kiwifruit bacterial canker severity. The diversity of soil microbial communities increased significantly after sulfur application in the range of 1.0~2.0 kg/m3. In particular, the bacterial genera level showed a high diversity after 2 years of sulfur application, reaching more than 516 genera. Furthermore, sulfur treatment resulted in a significant increase in specific microbial taxa, including members of the Acidothermus, norank_f__HSB_OF53-F07, and norank_f __Acidobacteriaceae__Subgroup_1. Moreover, the proportion of the dominant bacteria Acidothermus in the population showed an increasing trend. Altogether, the sulfur application is the key factor leading to microbial differences in kiwifruit rhizosphere soil. Appropriate sulfur can improve microbial structure characteristics of kiwifruit rhizosphere soil, increase bacterial diversity index, and change bacterial community composition structure.

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Lscβ and lscγ, two novel levansucrases of Pseudomonas syringae pv. actinidiae biovar 3, the causal agent of bacterial canker of kiwifruit, show different enzymatic properties

Cited by 15 publications

References 56 publications

A Chromatic Enzymatic Time-Temperature Integrator Device Based on the Degradation of Phenolic Compounds for the Real-Time Prediction of the Quality and Shelf Life of Cherries

A Chromatic Enzymatic Time-Temperature Integrator Device Based on the Degradation of Phenolic Compounds for the Real-Time Prediction of the Quality and Shelf Life of Cherries

Complete genome assembly of the levan‐positive strain PVFi1 of Pseudomonas savastanoi pv. savastanoi isolated from olive knots in Central Italy

Response of Soil Microbial Community Structure Mediated by Sulfur-Induced Resistance Against Kiwifruit Bacterial Canker

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