TaAP2-15, An AP2/ERF Transcription Factor, Is Positively Involved in Wheat Resistance to Puccinia striiformis f. sp. tritici

Hawku, Mehari Desta; Goher, Farhan; Islam, Ashraful; Guo, Jia; He, Fuxin; Bai, Xingxuan; Yuan, Pu; Kang, Zhensheng; Guo, Jun

doi:10.3390/ijms22042080

Cited by 21 publications

(12 citation statements)

References 70 publications

(39 reference statements)

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“…The promoters of DoAP2 genes contain endosperm-specific cis -acting elements with expression in the meristem in response to growth and developmental processes, and that respond to multiple stress signals, which might regulate various biological processes. This is consistent with the basic functions of AP2 TFs [ 37 , 49 ]. Different DoAP2 TFs play a variety of roles that may be related to their specific and/or differential binding to different cis -acting elements or other proteins, suggesting their involvement in different regulatory processes [ 50 , 51 , 52 ].…”

Section: Discussionsupporting

confidence: 90%

Section: Bioinformatics Analysis Of Doap2 Tfssupporting

confidence: 87%

“…AP2 responds to abiotic stresses [ 37 ]. To further investigate the changes in expression of DoAP2 genes to different abiotic stress treatments (cold, PEG and NaCl), we examined the expression levels of 14 DoAP2 genes under abiotic stress using qRT-PCR, while samples from untreated plantlets served as the control ( Figure 6 ).…”

Section: Resultsmentioning

confidence: 99%

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Genome-Wide Identification and Analysis of the APETALA2 (AP2) Transcription Factor in Dendrobium officinale

Zeng

Silva

Zhang

et al. 2021

IJMS

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The APETALA2 (AP2) transcription factors (TFs) play crucial roles in regulating development in plants. However, a comprehensive analysis of the AP2 family members in a valuable Chinese herbal orchid, Dendrobium officinale, or in other orchids, is limited. In this study, the 14 DoAP2 TFs that were identified from the D. officinale genome and named DoAP2-1 to DoAP2-14 were divided into three clades: euAP2, euANT, and basalANT. The promoters of all DoAP2 genes contained cis-regulatory elements related to plant development and also responsive to plant hormones and stress. qRT-PCR analysis showed the abundant expression of DoAP2-2, DoAP2-5, DoAP2-7, DoAP2-8 and DoAP2-12 genes in protocorm-like bodies (PLBs), while DoAP2-3, DoAP2-4, DoAP2-6, DoAP2-9, DoAP2-10 and DoAP2-11 expression was strong in plantlets. In addition, the expression of some DoAP2 genes was down-regulated during flower development. These results suggest that DoAP2 genes may play roles in plant regeneration and flower development in D. officinale. Four DoAP2 genes (DoAP2-1 from euAP2, DoAP2-2 from euANT, and DoAP2-6 and DoAP2-11 from basal ANT) were selected for further analyses. The transcriptional activation of DoAP2-1, DoAP2-2, DoAP2-6 and DoAP2-11 proteins, which were localized in the nucleus of Arabidopsis thaliana mesophyll protoplasts, was further analyzed by a dual-luciferase reporter gene system in Nicotiana benthamiana leaves. Our data showed that pBD-DoAP2-1, pBD-DoAP2-2, pBD-DoAP2-6 and pBD-DoAP2-11 significantly repressed the expression of the LUC reporter compared with the negative control (pBD), suggesting that these DoAP2 proteins may act as transcriptional repressors in the nucleus of plant cells. Our findings on AP2 genes in D. officinale shed light on the function of AP2 genes in this orchid and other plant species.

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Section: Discussionsupporting

confidence: 90%

Section: Bioinformatics Analysis Of Doap2 Tfssupporting

confidence: 87%

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Genome-Wide Identification and Analysis of the APETALA2 (AP2) Transcription Factor in Dendrobium officinale

Zeng

Silva

Zhang

et al. 2021

IJMS

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“…In wheat, an AP2 homolog, TaAP2-15 , positively regulated wheat resistance to Puccinia striiformis f. sp. tritici [ 62 ]. TaAP2-15 expression spiked at the onset of infection (12 hpi) and then again at 48 hpi, corresponding to the activation of signalling at the onset of infection and the colonisation of the host plant by the fungus, respectively.…”

Section: Discussionmentioning

confidence: 99%

Fusarium Yellows of Ginger (Zingiber officinale Roscoe) Caused by Fusarium oxysporum f. sp. zingiberi Is Associated with Cultivar-Specific Expression of Defense-Responsive Genes

et al. 2023

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Ginger (Zingiber officinale Roscoe) is an important horticultural crop, valued for its culinary and medicinal properties. Fusarium yellows of ginger, caused by Fusarium oxysporum f. sp. zingiberi (Foz), is a devastating disease that has significantly reduced the quality and crop yield of ginger worldwide. The compatible interaction between ginger and Foz leading to susceptibility is dissected here. The pathogenicity of two Foz isolates on ginger was confirmed by their ability to colonise ginger and in turn induce both internal and external plant symptoms typical of Fusarium yellows. To shed light on Foz susceptibility at the molecular level, a set of defense-responsive genes was analysed for expression in the roots of ginger cultivars challenged with Foz. These include nucleotide-binding site (NBS) type of resistant (R) genes with a functional role in pathogen recognition, transcription factors associated with systemic acquired resistance, and enzymes involved in terpenoid biosynthesis and cell wall modifications. Among three R genes, the transcripts of ZoNBS1 and ZoNBS3 were rapidly induced by Foz at the onset of infection, and the expression magnitude was cultivar-dependent. These expression characteristics extend to the other genes. This study is the first step in understanding the mechanisms of compatible host–pathogen interactions in ginger.

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“…Many AP2/ERF transcription factors regulate plant growth and development and stress response by interacting with other transcription factors, such as BR regulated ( Xie et al., 2019 ), WRKY ( Tripathi et al., 2015 ), MYB ( Zeng et al., 2015 ), and zinc finger transcription factors ( Hawku et al., 2021 ). One of the WRKY transcription factor, OsWRKY21 (LOC_Os01g60640) has been reported to be involved in rice growth and development by regulating the expression of GA metabolism and cell wall biosynthesis-related genes.…”

Section: Introductionmentioning

confidence: 99%

OsDREB2B, an AP2/ERF transcription factor, negatively regulates plant height by conferring GA metabolism in rice

Jin

et al. 2022

Front. Plant Sci.

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The AP2/ERF family is a large group of plant-specific transcription factors that play an important role in many biological processes, such as growth, development, and abiotic stress responses. OsDREB2B, a dehydration responsive factor (DRE/CRT) in the DREB subgroup of the AP2/ERF family, is associated with abiotic stress responses, such as cold, drought, salt, and heat stress, in Arabidopsis or rice. However, its role in regulating plant growth and development in rice is unclear. In this study, we reported a new function of OsDREB2B, which negatively regulates plant height in rice. Compared with wild type (WT), OsDREB2B-overexpressing (OE) rice exhibited dwarf phenotypes, such as reduction in plant height, internode length, and seed length, as well as grain yield, while the knockout mutants developed by CRISPR/Cas9 technology exhibited similar phenotypes. Spatial expression analysis revealed that OsDREB2B was highly expressed in the leaf sheaths. Under exogenous GA3 application, OsDREB2B expression was induced, and the length of the second leaf sheath of the OsDREB2B-OE lines recovered to that of the WT. OsDREB2B localized to the nucleus of the rice protoplast acted as a transcription activator and upregulated OsAP2-39 by directly binding to its promoter. OsDREB2B-OE lines reduced endogenous bioactive GA levels by downregulating seven GA biosynthesis genes and upregulating eight GA deactivation genes but not GA signaling genes. The yeast two-hybrid assay and bimolecular fluorescence complementation assay showed that OsDREB2B interacted with OsWRKY21. In summary, our study suggests that OsDREB2B plays a negative role in rice growth and development by regulating GA metabolic gene expression, which is mediated by OsAP2-39 and OsWRKY21, thereby reducing GA content and rice plant height.

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TaAP2-15, An AP2/ERF Transcription Factor, Is Positively Involved in Wheat Resistance to Puccinia striiformis f. sp. tritici

Cited by 21 publications

References 70 publications

Genome-Wide Identification and Analysis of the APETALA2 (AP2) Transcription Factor in Dendrobium officinale

Genome-Wide Identification and Analysis of the APETALA2 (AP2) Transcription Factor in Dendrobium officinale

Fusarium Yellows of Ginger (Zingiber officinale Roscoe) Caused by Fusarium oxysporum f. sp. zingiberi Is Associated with Cultivar-Specific Expression of Defense-Responsive Genes

OsDREB2B, an AP2/ERF transcription factor, negatively regulates plant height by conferring GA metabolism in rice

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