Biological characteristics and salt-tolerant plant growth-promoting effects of an ACC deaminase-producing Burkholderia pyrrocinia strain isolated from the tea rhizosphere

Han, Lizhen; Hong, Zhang; Yu, Xu; Li, Ying; Zhou, Jing

doi:10.1007/s00203-021-02204-x

Cited by 24 publications

(16 citation statements)

References 83 publications

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“…Previously, endophytic B. pyrrocinia JK-SH007 was reported to promote plant growth and suppress poplar stem canker disease (Ren et al, 2011). Burkholderia pyrrocinia P10 has also been shown to significantly enhance peanut seedling growth under saline conditions (100 and 170 mmol/l NaCl) by secreting IAA, solubilizing phosphorus compounds, and producing siderophores and ACC deaminase (Han et al, 2021). Thus, B. pyrrocinia shows potential as a PGPR or biocontrol candidate via direct interaction with plants.…”

Section: Discussionmentioning

confidence: 99%

Volatile organic compounds emitted by Burkholderia pyrrocinia CNUC9 trigger induced systemic salt tolerance in Arabidopsis thaliana

et al. 2022

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Salinity is among the most significant abiotic stresses that negatively affects plant growth and agricultural productivity worldwide. One ecofriendly tool for broadly improving plant tolerance to salt stress is the use of bio-inoculum with plant growth-promoting rhizobacteria (PGPR). In this study, a bacterium strain CNUC9, which was isolated from maize rhizosphere, showed several plant growth-promoting characteristics including the production of 1-aminocyclopropane-1-carboxylate deaminase, indole acetic acid, siderophore, and phosphate solubilization. Based on 16S rRNA and recA gene sequence analysis, we identified strain CNUC9 as Burkholderia pyrrocinia. Out of bacterial determinants to elicit plant physiological changes, we investigated the effects of volatile organic compounds (VOCs) produced by B. pyrrocinia CNUC9 on growth promotion and salinity tolerance in Arabidopsis thaliana. Higher germination and survival rates were observed after CNUC9 VOCs exposure under 100 mM NaCl stress. CNUC9 VOCs altered the root system architecture and total leaf area of A. thaliana compared to the control. A. thaliana exposed to VOCs induced salt tolerance by increasing its total soluble sugar and chlorophyll content. In addition, lower levels of reactive oxygen species, proline, and malondialdehyde were detected in CNUC9 VOCs-treated A. thaliana seedlings under stress conditions, indicating that VOCs emitted by CNUC9 protected the plant from oxidative damage induced by salt stress. VOC profiles were obtained through solid-phase microextraction and analyzed by gas chromatography coupled with mass spectrometry. Dimethyl disulfide (DMDS), methyl thioacetate, and 2-undecanone were identified as products of CNUC9. Our results indicate that optimal concentrations of DMDS and 2-undecanone promoted growth in A. thaliana seedlings. Our findings provide greater insight into the salt stress alleviation of VOCs produced by B. pyrrocinia CNUC9, as well as potential sustainable agriculture applications.

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Section: Discussionmentioning

confidence: 99%

Volatile organic compounds emitted by Burkholderia pyrrocinia CNUC9 trigger induced systemic salt tolerance in Arabidopsis thaliana

et al. 2022

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“…As a result, ACCD produced by PGPR shields the plants from the damaging effects of ethylene when they are exposed to abiotic stresses (Sapre et al, 2019 ). Several researchers have found that halotolerant PGPR strains exhibited the production of ACC deaminase when exposed to a harsher saline environment (Wu et al, 2012 ; Sarkar et al, 2018 ; Ji et al, 2020 ; Han et al, 2021 ). Like our study, salinity-alleviating Bacillus sp.…”

Section: Resultsmentioning

confidence: 99%

PGPR Kosakonia Radicincitans KR-17 Increases the Salt Tolerance of Radish by Regulating Ion-Homeostasis, Photosynthetic Molecules, Redox Potential, and Stressor Metabolites

et al. 2022

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Among abiotic stresses, salinity is a significant limiting factor affecting agricultural productivity, survival, and production, resulting in significant economic losses. Considering the salinity problem, the goal of this study was to identify a halotolerant beneficial soil bacterium to circumvent salinity-induced phytotoxicity. Here, strain KR-17 (having an irregular margin; a mucoid colony; Gm-ve short rod; optimum temperature, 30°C; pH 7.0; no any pigmentation; showed a positive response to citrate utilization, catalase, starch, sucrose, lactose, and dextrose, etc.) recovered from rhizosphere soils of the potato-cultivating field, tolerated surprisingly a high (18% NaCl; 3.-M concentration) level of salt and identified as Kosakonia radicincitans (Accession No. OM348535). This strain was discovered to be metabolically active, synthesized essential PGP bioactive molecules like indole-3-acetic acid (IAA), siderophore (iron-chelating compounds), ACC deaminase, and ammonia, the quantity of which, however, increased with increasing NaCl concentrations. Here, Raphanus sativus L. (radish) was taken as a model crop to evaluate the adverse impact of NaCl, as well as salinity alleviation by halotolerant K. radicincitans. Salinity-induced toxicity to R. sativus was increased in a dose-dependent way, as observed both in vitro and in vivo conditions. Maximum NaCl levels (15%) demonstrated more extreme harm and considerably reduced the plant's biological features. However, membrane damage, relative leaf water content (RLWC), stressor metabolites, and antioxidant enzymes were increased as NaCl concentration increased. In contrast, halotolerant K. radicincitans KR-17 relieved salinity stress and enhanced the overall performance of R. sativus (L.) by increasing germination efficiency, dry biomass, and leaf pigments even in salt-challenged conditions. Additionally, KR-17 inoculation significantly (p ≤ 0.05) improved plant mineral nutrients (Na, K, Ca, Mg, Zn, Fe, Cu, P, and N). Following inoculation, strain KR-17 enhanced the protein, carbohydrates, root pigments, amino acids (AsA and Lys), lipids, and root alkaloids in R. sativus (L.). Besides these, due to PGPR seed priming in NaCl-stressed/non-stressed conditions, membrane damage, RLWC, stressor metabolites, and antioxidant defense enzymes were dramatically reduced. The strong biofilm-forming capacity of K. radicincitans could result in both in vitro and in vivo colonization under NaCl stress. Conclusively, halotolerant K. radicincitans KR-17 may probably be investigated affordably as the greatest way to increase the production of radish under salinity-stressed soils.

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“…PGPR is essential for improved plant growth, either through the direct secretion of plant growth and development-related substances or indirectly by regulating the living plant environment [ 45 ]. ACC deaminase-producing Burkholderia cepacia P10 promotes plant growth by producing compounds such as IAA and iron carriers [ 46 ]. Two PGPR strains from the pokeweed root zone, WM13-24 and M30-35, promote ryegrass growth and root development by regulating phytohormone distribution [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolites from Bacillus subtilis J-15 Affect Seedling Growth of Arabidopsis thaliana and Cotton Plants

Zhang

Yang

Zhao

et al. 2022

Plants

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Bacillus subtilis J-15 is a plant growth-promoting rhizobacteria isolated from the soil rhizosphere of cotton and is resistant to cotton verticillium wilt. This study evaluated the effects of metabolites of J-15 (J-15-Ms), including mycosubtilin, on plant growth using Arabidopsis and cotton plants. The results showed that J-15-Ms promoted Arabidopsis seeding growth at lower concentrations of 0.2 μg/mL but inhibited the growth at higher concentrations, such as 20 μg/mL. Similar results were obtained in cotton. Thus, J-15-Ms-treated plants showed low-concentration-induced growth promotion and high-concentration-induced growth inhibition. The J-15-Ms components were analyzed by liquid chromatography–mass spectrometry. Correlation analysis using the J-15 genomic databases suggested that J-15 may synthesize indoleacetic acid via the indole-3-pymvate pathway and indole-3-acetamide pathway. Treatment with mycosubtilin, a purified peptide from J-15-Ms, showed that the peptide promoted Arabidopsis growth at a low concentration (0.1 μg/mL) and inhibited plant growth at high concentrations (higher than 1 μg/mL), which also significantly increased plant lateral root number. Transcriptomic analysis showed that mycosubtilin might promote lateral root development and inhibit plant primary root growth by regulating the expression of the plant hormone signaling pathway. This study reveals the mechanism of Bacillus subtilis J-15 in affecting plant growth.

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Biological characteristics and salt-tolerant plant growth-promoting effects of an ACC deaminase-producing Burkholderia pyrrocinia strain isolated from the tea rhizosphere

Cited by 24 publications

References 83 publications

Volatile organic compounds emitted by Burkholderia pyrrocinia CNUC9 trigger induced systemic salt tolerance in Arabidopsis thaliana

Volatile organic compounds emitted by Burkholderia pyrrocinia CNUC9 trigger induced systemic salt tolerance in Arabidopsis thaliana

PGPR Kosakonia Radicincitans KR-17 Increases the Salt Tolerance of Radish by Regulating Ion-Homeostasis, Photosynthetic Molecules, Redox Potential, and Stressor Metabolites

Metabolites from Bacillus subtilis J-15 Affect Seedling Growth of Arabidopsis thaliana and Cotton Plants

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