Multi-biomarker assessment in the giant freshwater prawn Macrobrachium rosenbergii after deltamethrin exposure

Jiang, Qun; Jiang, Ziyan; Ao, Shiqi; Gao, Xiaojian; Zhu, Xinhai; Zhang, Zirui; Zhang, Xiaojun

doi:10.1016/j.ecoenv.2021.112067

Cited by 16 publications

(6 citation statements)

References 40 publications

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“…ACP and AKP are two common immune-related enzymes, both of which are involved in the detoxification process of the organism when aquatic organisms are affected by external environmental factors (Chi et al, 2019). The study about the ACP and AKP activities in deltamethrin stressed Macrobrachium rosenbergii found that the ACP activity showed a wavy change and the AKP activity showed a trend of increasing and then decreasing after acute stress (Guo et al, 2021;Jiang et al, 2021). A similar conclusion was reached by Peng., in his study on the acute toxicity of the proposed cave green crab (Cyprinus carpio) (Peng et al, 2018).…”

Section: Effects Of Residual Chlorine On Physiological Metabolism Of ...supporting

confidence: 54%

Transcriptome reveals the immune and antioxidant effects of residual chlorine stress on Cyclina sinensis

Wang¹,

Ren²,

Li³

et al. 2023

Front. Mar. Sci.

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Residual chlorine is a common by-product of warm drainage in coastal nuclear power plants. when accumulating to some limit, it may threaten marine ecosystem especially for benthic clam. However, there are few studies on the molecular mechanisms related to immunity and antioxidant of residual chlorine stress on clams. In this study, the clam (Cyclina sinensis) was exposed for 96 h at different concentrations (0, 50, 100, 150, 200, 250, 300, 350, 400, 450 and 500 mg/L) of residual chlorine to observe its mortality, measure the activity of antioxidant and immune-related enzymes, and analyses the gene expression level in the hepatopancreas by using the transcriptome sequencing. The results showed that the mortality rate increased with the increase of stress time and concentration, and the mortality rate in the 400, 450 and 500 mg/L groups reached 100% at 96 h. The tolerance to residual chlorine of C. sinensis decreased with the increase of chlorine dioxide concentration, and the LC50 of 96 h was 217.6 mg/L by linear regression method. After residual chlorine stress, the activity of antioxidant-related enzymes (T-AOC and SOD) in the hepatopancreas showed a trend of first increase and then decrease with the extension of stress time. The immune-related enzyme activities of AKP and LZM showed a downward trend between 0 and 96 h, while the ACP enzyme activity showed a trend of first rising and then decreasing. Transcriptome analysis showed that residual chlorine stress significantly changed the expression levels of immune-related molecules associated with signal transduction, prophenoloxidase cascade, cell apoptosis and pattern recognition protein/receptor. Moreover, glutathione S-transferase (GST), heat shock protein (HSP) and other antioxidant-related genes were significantly affected under residual chlorine stress. This study provided valuable information for understanding the effects of residual chlorine stress on survival, physiological metabolism and molecular mechanisms of immune and antioxidant functions of C. sinensis.

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Section: Effects Of Residual Chlorine On Physiological Metabolism Of ...supporting

confidence: 54%

Transcriptome reveals the immune and antioxidant effects of residual chlorine stress on Cyclina sinensis

Wang¹,

Ren²,

Li³

et al. 2023

Front. Mar. Sci.

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“…Among pyrethroids, DLM is considered one of the safest available insecticides 3 . However, numerous recent reports indicate that DLM is an environmental and industrial pollutant and it is toxic to non‐aimed organisms living in the same ecosystem 4,5 . DLM can induce neurotoxicity in many species including human, and DLM exposure is related to neurodegenerative diseases 6,7 …”

Section: Introductionmentioning

confidence: 99%

Deltamethrin induces apoptosis in cerebrum neurons of quail via promoting endoplasmic reticulum stress and mitochondrial dysfunction

Han

et al. 2022

Environmental Toxicology

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Deltamethrin (DLM) is a widely used and highly effective insecticide. DLM exposure is harmful to animal and human. Quail, as a bird model, has been widely used in the field of toxicology. However, there is little information available in the literature about quail cerebrum damage caused by DLM. Here, we investigated the effect of DLM on quail cerebrum neurons. Four groups of healthy quails were assigned (10 quails in each group), respectively given 0, 15, 30, and 45 mg/kg DLM by gavage for 12 weeks. Through the measurements of quail cerebrum, it was found that DLM exposure induced obvious histological changes, oxidative stress, and neurons apoptosis. To further explore the possible molecular mechanisms, we performed real-time quantitative PCR to detect the expression of endoplasmic reticulum (ER) stressrelated mRNA such as glucose regulated protein 78 kD, activating transcription factor 6, inositol requiring enzyme, and protein kinase RNA (PKR)-like ER kinase. In addition, we detected ATP content in quail cerebrum to evaluate the functional status of mitochondria. The study showed that DLM exposure significantly increased the expression of ER stress-related mRNA and decreased ATP content in quail cerebrum tissues. These results suggest that chronic exposure to DLM induces apoptosis of quail cerebrum neurons via promoting ER stress and mitochondrial dysfunction. Furthermore, our results provide a novel explanation for DLM-induced apoptosis of avian cerebrum neurons.

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“…During this process, host cells will produce abundant ROS, such as superoxide anion (O 2 •− ), hydrogen peroxide (H 2 O 2 ), hydroxyl radicals ( • OH), and singlet oxygen ( 1 O 2 ), and reactive oxygen intermediates to kill the invading pathogens; however, excessive levels of ROS in the body will also cause the destruction and damage of DNA and other biological macromolecules [ 18 ]. Crustaceans can rely on complex enzymatic antioxidant systems to alleviate and restore the oxidative damage, SOD, GPx, GST, CAT, MDA, and so on [ 61 ]. SODs conduct the first step in eliminating the oxidative damage caused by ROS from cells.…”

Section: Discussionmentioning

confidence: 99%

A Novel Disease (Water Bubble Disease) of the Giant Freshwater Prawn Macrobrachium rosenbergii Caused by Citrobacter freundii: Antibiotic Treatment and Effects on the Antioxidant Enzyme Activity and Immune Responses

et al. 2022

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The giant freshwater prawn, Macrobrachium rosenbergii, is an important and economical aquaculture species widely farmed in tropical and subtropical areas of the world. A new disease, “water bubble disease (WBD)”, has emerged and resulted in a large loss of M. rosenbergii cultured in China. A water bubble with a diameter of about 7 mm under the carapace represents the main clinical sign of diseased prawns. In the present study, Citrobacter freundii was isolated and identified from the water bubble. The optimum temperature, pH, and salinity of the C. freundii were 32 °C, 6, and 1%, respectively. A challenging experiment showed that C. freundii caused the same typical signs of WBD in prawns. Median lethal dose of the C. freundii to prawn was 104.94 CFU/g. According to the antibiogram tests of C. freundii, florfenicol and ofloxacin were selected to evaluate their therapeutic effects against C. freundii in prawn. After the challenge with C. freundii, 86.67% and 72.22% survival of protective effects against C. freundii were evaluated in the oral florfenicol pellets and oral ofloxacin pellets feding prawns, respectively, whereas the mortality of prawns without fed antibiotics was 93%. After antibiotic treatment and C. freundii infection, the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), malondialdehyde (MDA), acid phosphatase (ACP), alkaline phosphatase (ALP), and lysozyme (LZM) in the hemolymph and hepatopancreas of the prawns and the immune-related gene expression levels of Cu/Zn-SOD, CAT, GPx, GST, LZM, ACP, anti-lipopolysaccharide factor, crustin, cyclophilin A, and C-type lectin in hepatopancreas were all significantly changed, indicating that innate immune responses were induced by C. freundii. These results can be beneficial for the prevention and control of C. freundii in prawns.

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Multi-biomarker assessment in the giant freshwater prawn Macrobrachium rosenbergii after deltamethrin exposure

Cited by 16 publications

References 40 publications

Transcriptome reveals the immune and antioxidant effects of residual chlorine stress on Cyclina sinensis

Transcriptome reveals the immune and antioxidant effects of residual chlorine stress on Cyclina sinensis

Deltamethrin induces apoptosis in cerebrum neurons of quail via promoting endoplasmic reticulum stress and mitochondrial dysfunction

A Novel Disease (Water Bubble Disease) of the Giant Freshwater Prawn Macrobrachium rosenbergii Caused by Citrobacter freundii: Antibiotic Treatment and Effects on the Antioxidant Enzyme Activity and Immune Responses

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