Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan

Rahman, Rafi U.; Souza, Bárbara Nayane Rosário Fernandes; Uddin, Izhar; Carrara, Luana; Brito, Luiz Paulo; Costa, Monique Melo; Mahmood, Muhammad Asif; Khan, Sozaina; Lima, José Bento Pereira; Martins, Ademir Jesús

doi:10.1038/s41598-021-83465-w

Cited by 32 publications

(25 citation statements)

References 70 publications

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“…Therefore, T1520I has been selected in populations with background kdr mutation F1534C established in the field [30]. Consistent with previous studies [29][30][31], a strong association of T1520I with F1534C was also observed in the Myanmar Ae. aegypti population analyzed in this study.…”

Section: Discussionsupporting

confidence: 79%

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Knockdown Resistance Mutations in the Voltage-Gated Sodium Channel of Aedes aegypti (Diptera: Culicidae) in Myanmar

Naw

Võ

Lê

et al. 2022

Insects

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Aedes aegypti is an important mosquito vector transmitting diverse arboviral diseases in Myanmar. Pyrethroid insecticides have been widely used in Myanmar as the key mosquito control measure, but the efforts are constrained by increasing resistance. Knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC) are related to pyrethroid resistance in Ae. aegypti. We analyzed the patterns and distributions of the kdr mutations in Ae. aegypti in the Mandalay area of Myanmar. The segment 6 regions of domains II and III of vgsc were separately amplified from individual Ae. aegypti genomic DNA via polymerase chain reaction. The amplified gene fragments were sequenced. High proportions of three major kdr mutations, including S989P (54.8%), V1016G (73.6%), and F1534C (69.5%), were detected in the vgsc of Ae. aegypti from all studied areas. Other kdr mutations, T1520I and F1534L, were also found. These kdr mutations represent 11 distinct haplotypes of the vgsc population. The S989P/V1016G/F1534C was the most prevalent, followed by S989P/V1016V and V1016G/F1534C. A quadruple mutation, S989P/V1016G/T1520I/F1534C, was also identified. High frequencies of concurrent kdr mutations were observed in vgsc of Myanmar Ae. aegypti, suggesting a high level of pyrethroid resistance in the population. These findings underscore the need for an effective vector control program in Myanmar.

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Section: Discussionsupporting

confidence: 79%

“…aegypti in this study was also a recently identified vgsc mutation in Ae. aegypti from India, Pakistan, and China [29][30][31], and most recently in Myanmar Ae. aegypti [21].…”

Section: Discussionmentioning

confidence: 99%

Knockdown Resistance Mutations in the Voltage-Gated Sodium Channel of Aedes aegypti (Diptera: Culicidae) in Myanmar

Naw

Võ

Lê

et al. 2022

Insects

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“…Overexpression of CYP9J28 and CYP4G16 mediates resistance to pyrethroids in Aedes aegypti and Aedes albopictus, respectively [18,19]. Transcriptomic analysis of Bombyx mandarina suggests that CYP306A mediates ecdysteroid production and insecticide metabolism [20].…”

Section: P450-mediated Insecticide Resistancementioning

confidence: 99%

“…Subsequently, excessive transcription of P450s were also found in resistant populations of A. aegypti [56], and CYP9J28 and CYP6BB2 were frequently detected in research on pyrethroid resistance [57]. Conversely, CYP9J28 showed the highest resistance to pyrethroids in Pakistan [19], while CYP9J32 showed the highest resistance to deltamethrin and cypermethrin in Thailand, Mexico, and Vietnam, CYP9J24 and CYP9J26 had the highest resistance to pyrethroids in Latin America, and CYP9M6 and CYP4D24 mediates resistance to pyrethroids in Asia [58,59]. Different Drosophila strains utilize different genes in DDT resistance.…”

Section: Evolutionary Adaptabilitymentioning

confidence: 99%

The Role of Insect Cytochrome P450s in Mediating Insecticide Resistance

Nayak

Xiong

et al. 2022

Agriculture

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In many organisms, cytochrome P450 enzymes are the primary detoxifying enzymes. Enhanced P450 activity can be mediated by the emergence of new genes, increased transcription due to mutations in the promoter regions, changes in enzyme structures and functions due to mutations in protein-coding regions, or changes in post-translational modifications; all of these changes are subject to insecticide selection pressure. Multiple signalling pathways and key effector molecules are involved in the regulation of insect P450s. Increased P450 activity is a key mechanism inducing insect resistance. Hence, downregulation of selected P450s is a promising strategy to overcome this resistance. Insect P450 inhibitors that act as insecticide synergists, RNA interference to induce P450 gene silencing, and the use of transgenic insects and crops are examples of strategies utilized to overcome resistance. This article reviews the latest advances in studies related to insect P450s-mediated agrochemical resistance, with focuses on the regulatory mechanisms and associated pest management strategies. Future investigations on the comprehensive regulatory pathways of P450-mediated detoxification, identification of key effectors, and downregulation strategies for P450s will ecologically, economically, and practically improve pest management.

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“…albopictus, treatments against this species have not provided the reduction in its populations to satisfyingly low levels so far. As a result of the persistent use of insecticides, this species has developed resistance to a wide range of pyrethroids [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. As conventional mosquito control has often failed to suppress Ae.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Aedes albopictus (Diptera: Culicidae) Mass Rearing through Cost-Effective Larval Feeding

Kavran

Puggioli

Šiljegović

et al. 2022

Insects

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Aedes (Stegomyia) albopictus (Skuse, 1895) is an invasive important medical and veterinary pest species. The sterile insect technique (SIT) involves the mass rearing of males, and their sterilization and release into the habitat to compete with wild males. Our research objective was to compare the effectiveness of three larval diet recipes (IAEA-BY, BCWPRL, and MIX-14) in the laboratory rearing of Ae. albopictus males to evaluate the available economical feeding alternatives. The separation of sexes was done in the pupal stage by sieving. Reared males were tested for flight capacity and longevity. The application of the BCWPRL diet resulted in a higher portion of sieved male pupae than females, but the development of males was the slowest, and the number of obtained males (pupae and adults) was lower compared to the other two diets. The adult mean survival time was the highest in males fed with MIX-14 and the lowest in males fed with IAEA-BY. Males fed by IAEA-BY also demonstrated higher initial mortality in the adult stage. The diets BCWPRL and MIX-14 are economically more convenient than IAEA-BY (2.28 and 5.30 times cheaper, respectively). The cheapest diet, MIX-14, might represent a candidate for replacing the effective but still expensive IAEA-BY larval diet, providing lower costs of sterile male production.

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Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan

Cited by 32 publications

References 70 publications

Knockdown Resistance Mutations in the Voltage-Gated Sodium Channel of Aedes aegypti (Diptera: Culicidae) in Myanmar

Knockdown Resistance Mutations in the Voltage-Gated Sodium Channel of Aedes aegypti (Diptera: Culicidae) in Myanmar

The Role of Insect Cytochrome P450s in Mediating Insecticide Resistance

Optimization of Aedes albopictus (Diptera: Culicidae) Mass Rearing through Cost-Effective Larval Feeding

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