Trichinella spiralis cystatin, TsCstN, modulates STAT4/IL-12 to specifically suppress IFN-γ production

Kobpornchai, Porntida; Tiffney, Ellen‐Alana; Adisakwattana, Poom; Flynn, Robin J.

doi:10.1016/j.cellimm.2021.104303

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How Does Trichinella Elude the Host Immun...1

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2024

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Cited by 3 publications

(1 citation statement)

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“…This would lead to a specific suppression of IFN-γ production, but not of IL-17A. In this way the parasite could evade the Th1-based immune response, facilitating the establishment of the muscle stage ( Kobpornchai et al, 2021 ).…”

Section: How Does Trichinella Elude the Host Immun...mentioning

confidence: 99%

Trichinella-induced immunomodulation: Another tale of helminth success

Bruschi

Ashour

Othman

2022

Food and Waterborne Parasitology

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Section: How Does Trichinella Elude the Host Immun...mentioning

confidence: 99%

Trichinella-induced immunomodulation: Another tale of helminth success

Bruschi

Ashour

Othman

2022

Food and Waterborne Parasitology

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Galla Turcica alleviates gingiva inflammation and alveolar bone resorption via regulating Th1/Th17 in a mouse model of periodontitis

Han,

Yu,

2024

ADV TRADIT MED (ADTM)

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Identification of the protease inhibitory domain of Trichinella spiralis novel cystatin (TsCstN)

Yuthithum,

Phuphisut,

Reamtong

et al. 2024

Parasites Hosts Dis

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The Trichinella spiralis novel cystatin (TsCstN) inhibits cathepsin L (CatL) activity and inflammation of macrophages during lipopolysaccharide (LPS) induction. To identify the protease inhibitory region, this study applied an in silico modeling approach to simulate truncation sites of TsCstN (Ts01), which created four truncated forms, including TsCstN∆1-39 (Ts02), TsCstN∆1-71 (Ts03), TsCstN∆1-20, ∆73-117 (Ts04), and TsCstN∆1-20, ∆42-117 (Ts05). The superimposition of these truncates modeled with AlphaFold Colab indicated that their structures were more akin to Ts01 than those modeled with I-TASSER. Moreover, Ts04 exhibited the closest resemblance to the structure of Ts01. The recombinant Ts01 (rTs01) and truncated proteins (rTs02, rTs03, and rTs04) were successfully expressed in a prokaryotic expression system while Ts05 was synthesized, with sizes of approximately 14, 12, 8, 10, and 2.5 kDa, respectively. When determining the inhibition of CatL activity, both rTs01 and rTs04 effectively reduced CatL activity in vitro. Thus, the combination of the α1 and L1 regions may be sufficient to inhibit CatL. This study provides comprehensive insights into TsCstN, particularly regarding its protein function and inhibitory domains against CatL.

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Trichinella spiralis cystatin, TsCstN, modulates STAT4/IL-12 to specifically suppress IFN-γ production

Cited by 3 publications

References 32 publications

Trichinella-induced immunomodulation: Another tale of helminth success

Trichinella-induced immunomodulation: Another tale of helminth success

Galla Turcica alleviates gingiva inflammation and alveolar bone resorption via regulating Th1/Th17 in a mouse model of periodontitis

Identification of the protease inhibitory domain of Trichinella spiralis novel cystatin (TsCstN)

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