Redesign of protein nanocages: the way from 0D, 1D, 2D to 3D assembly

Lv, Chenyan; Zhang, Xiaorong; Liu, Yu; Zhang, Tuo; Chen, Hai; Zang, Jiachen; Zheng, Bowen; Zhao, Guanghua

doi:10.1039/d0cs01349h

Cited by 49 publications

(24 citation statements)

References 249 publications

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“…Diverse biological and engineered protein compartments and cages have been repurposed for biomedical applications, as composite nanomaterials, for bioremediation and biosensing and to a much lesser extent, for in vitro enzyme catalysis and as in vivo nanoreactors [124,[162][163][164]. Biological compartments large enough for multi-enzyme encapsulation include the eukaryotic major vault particles, bacterial microcompartments (~50-200 nm), encapsulins (~20-40 nm), and lumazine synthase (~15 nm) (Figure 5).…”

Section: Encapsulation In Proteinaceous Compartments Cages and Virus-like Particlesmentioning

confidence: 99%

Organizing Multi-Enzyme Systems into Programmable Materials for Biocatalysis

Seo

Schmidt‐Dannert

2021

Catalysts

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Significant advances in enzyme discovery, protein and reaction engineering have transformed biocatalysis into a viable technology for the industrial scale manufacturing of chemicals. Multi-enzyme catalysis has emerged as a new frontier for the synthesis of complex chemicals. However, the in vitro operation of multiple enzymes simultaneously in one vessel poses challenges that require new strategies for increasing the operational performance of enzymatic cascade reactions. Chief among those strategies is enzyme co-immobilization. This review will explore how advances in synthetic biology and protein engineering have led to bioinspired co-localization strategies for the scaffolding and compartmentalization of enzymes. Emphasis will be placed on genetically encoded co-localization mechanisms as platforms for future autonomously self-organizing biocatalytic systems. Such genetically programmable systems could be produced by cell factories or emerging cell-free systems. Challenges and opportunities towards self-assembling, multifunctional biocatalytic materials will be discussed.

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Section: Encapsulation In Proteinaceous Compartments Cages and Virus-like Particlesmentioning

confidence: 99%

Organizing Multi-Enzyme Systems into Programmable Materials for Biocatalysis

Seo

Schmidt‐Dannert

2021

Catalysts

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“…However, a universal theoretical approach toward computational catalysis with generally applicable algorithms is not available. This can be a handicap for practical applications, especially in view of the growing field of experimental catalysis with increasingly complex catalyst structures such as metalorganic frameworks [34][35][36][37], single-atom catalysts [38][39][40], supported nanoparticles [41], supported organometallic catalysts [42][43][44][45], binary catalysts [46], encapsulated catalysts [47][48][49][50], self-assembling nanostructures [51,52], nanozymes [53], protein nanocages [54], nucleic-acid catalysts [55], and artificial (metallo)enzymes [56][57][58][59].…”

Section: Introductionmentioning

confidence: 99%

Autonomous Reaction Network Exploration in Homogeneous and Heterogeneous Catalysis

Steiner

Reiher

2022

Top Catal

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Autonomous computations that rely on automated reaction network elucidation algorithms may pave the way to make computational catalysis on a par with experimental research in the field. Several advantages of this approach are key to catalysis: (i) automation allows one to consider orders of magnitude more structures in a systematic and open-ended fashion than what would be accessible by manual inspection. Eventually, full resolution in terms of structural varieties and conformations as well as with respect to the type and number of potentially important elementary reaction steps (including decomposition reactions that determine turnover numbers) may be achieved. (ii) Fast electronic structure methods with uncertainty quantification warrant high efficiency and reliability in order to not only deliver results quickly, but also to allow for predictive work. (iii) A high degree of autonomy reduces the amount of manual human work, processing errors, and human bias. Although being inherently unbiased, it is still steerable with respect to specific regions of an emerging network and with respect to the addition of new reactant species. This allows for a high fidelity of the formalization of some catalytic process and for surprising in silico discoveries. In this work, we first review the state of the art in computational catalysis to embed autonomous explorations into the general field from which it draws its ingredients. We then elaborate on the specific conceptual issues that arise in the context of autonomous computational procedures, some of which we discuss at an example catalytic system. Graphical Abstract

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“…Thus, proteinbased nanoparticles have been widely used as a delivery platform for various vaccines and drugs. A more detailed description of the biological function, structure, and geometry of various protein nanoparticles and their application in nanomedicine, including the development of other vaccines, are described in excellent recent reviews [40][41][42][43][44][45][46]. This review mainly focuses on recent innovations in protein-based nanoparticle vaccines for protection against SARS-CoV-2 (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Protein-Based Nanoparticle Vaccines for SARS-CoV-2

Sung

Kim

Lee

et al. 2021

IJMS

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The pandemic caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has upended healthcare systems and economies around the world. Rapid understanding of the structural biology and pathogenesis of SARS-CoV-2 has allowed the development of emergency use or FDA-approved vaccines and various candidate vaccines. Among the recently developed SARS-CoV-2 candidate vaccines, natural protein-based nanoparticles well suited for multivalent antigen presentation and enhanced immune stimulation to elicit potent humoral and cellular immune responses are currently being investigated. This mini-review presents recent innovations in protein-based nanoparticle vaccines against SARS-CoV-2. The design and strategy of displaying antigenic domains, including spike protein, receptor-binding domain (RBD), and other domains on the surface of various protein-based nanoparticles and the performance of the developed nanoparticle-based vaccines are highlighted. In the final part of this review, we summarize and discuss recent advances in clinical trials and provide an outlook on protein-based nanoparticle vaccines.

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Redesign of protein nanocages: the way from 0D, 1D, 2D to 3D assembly

Abstract: This review focuses on the design and construction of artificial protein nanocages, and their assembly into highly ordered supramolecules.

Cited by 49 publications

References 249 publications

Organizing Multi-Enzyme Systems into Programmable Materials for Biocatalysis

Organizing Multi-Enzyme Systems into Programmable Materials for Biocatalysis

Autonomous Reaction Network Exploration in Homogeneous and Heterogeneous Catalysis

Protein-Based Nanoparticle Vaccines for SARS-CoV-2

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