Pollen micromorphology of eastern Chinese Polygonatum and its role in taxonomy by using scanning electron microscopy

Abstract

Although considerable progress has recently been made in the taxonomic study of Asparagaceae, but the Polygonatum remains one of the most poorly investigated genus. Therefore, the present study was designed with the aim, to document the pollen micromorphology of closely related Polygonatum taxa and its taxonomic significance. Pollen micro‐morphological features were observed using scanning electron microscopy—SEM). The pollen grains are monad, navicular, monocolpate, and radially symmetrical. A significant variation was observed in the exine sculpturing. Moreover, most of the Polygonatum taxa have perforate pollen while some of them were mixed with psilate to perforate, perforate to microreticulate, sometimes scabrate, gemmate with baculate. Hence, this study documented the pollen morphology of Polygonatum taxa and strengthens the taxonomic identification of the genus Polygonatum based on pollen characters, which helps and can be used as an additional tool for the correct identification and discrimination of the species of Polygonatum at generic and species level.

Hepatoprotective Screening of Seriphidium kurramense (Qazilb.) Y.R. Ling

“…Sodium arsenate treatment concentration depends on increased oxidative stress in the tissues, leading to abnormal oocytes with damaged DNA. And also, it is recommended to study the advanced techniques using SEM analysis for future study [ 38 , 39 ]. However, in the present investigation, SKM treatment showed an increase in comet head length, DNA percentage, and reduction of comet length, tail length, and moment, as well as DNA percentage in the tail of the comet, suggesting its protecting effects against CCl4-induced cellular toxicity by preventing DNA damage.…”

Pollen morphology and its taxonomic potential in some selected taxa of Caesalpiniaceae observed under light microscopy and scanning electron microscopy

“…and Cassia L. belonging to subfamily Caesalpiniaceae are symmetrical, trizonocolporate, isopolar, and the shape varies from oblate spheroidal to prolate (Mishra & Srivastava, 2015). Moreover, the advanced techniques of LM and SEM can not only play a key role in this field to observe the ultrastructure of plant material (Ali et al, 2021;Ashfaq et al, 2020;Bahadur et al, 2020;Butt et al, 2021;Gul et al, 2019Gul et al, , 2021 but also in other filed of biological sciences (Hameed et al, 2021;Javed et al, 2021;Nabila et al, 2021;Rasool et al, 2021;Rubab et al, 2020Rubab et al, , 2021. and Lakki Marwat (Figures 1 and 2; Table 1). F I G U R E 2 Flower pictures of studied species of Caesalpiniaceae (a) Bauhinia variegata, (b) Caesalpinia pulcherrima, (c) Delonix regia (d) Parkinsonia aculeata, (e) Saraca indica, (f) Senna corymbosa, (g) Senna obtusifolia, (h) Senna occedantalis, (i) Senna surattensis, and (j) Cassia fistula LM of pollen was carried out in the anatomy lab.…”

“…and Cassia L. belonging to subfamily Caesalpiniaceae are symmetrical, trizonocolporate, isopolar, and the shape varies from oblate spheroidal to prolate (Mishra & Srivastava, 2015). Moreover, the advanced techniques of LM and SEM can not only play a key role in this field to observe the ultrastructure of plant material (Ali et al, 2021; Ashfaq et al, 2020; Bahadur et al, 2020; Butt et al, 2021; Gul et al, 2019, 2021) but also in other filed of biological sciences (Hameed et al, 2021; Javed et al, 2021; Nabila et al, 2021; Rasool et al, 2021; Rubab et al, 2020, 2021).…”

Toxicological effects of the chemical and green ZnO NPs on Cyprinus carpio L. observed under light and scanning electron microscopy

“…Seaweed Sargassum myriocystum (microalgae) is used for the synthesis of ZnO NPs (Zafar et al, 2020). Furthermore, light microscopy, scanning electron microscope (SEM), X-ray diffraction (XRD), and Fourier transmission infrared radiation (FTIR) techniques have not only significant role in the characterization of nanoparticles but can also be used to analyze the fine detail of plant material (Ali et al, 2021;Ashfaq et al, 2020;Bahadur et al, 2020;Butt et al, 2021;Gul et al, 2019Gul et al, , 2021Shuaib, Bahadur, & Hussain, 2020;Rubab et al, 2020) and in other field of the diverse biological research as well (Hameed et al, 2021;Javed et al, 2021;Nabila et al, 2021;Rubab et al, 2021).…”