Mass spectrometry-based proteomic analysis of potential infectious bursal disease virus VP3-interacting proteins in chicken embryo fibroblasts cells

Ma, Sunting; Wang, Yong-Shan; Wang, Xiaoli; Xia, Xing-Xia; Bi, Zhenwei; Wang, Jingyu; Zhu, Yimin; Ouyang, Wei; Qian, Jing

doi:10.1007/s11262-021-01828-x

Cited by 3 publications

(4 citation statements)

References 29 publications

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“…While the de novo synthesized RNA detected by this protocol could be replicative intermediates or genomic strands, they could also be molecules resembling mRNA that are destined for translation. As previously discussed, ribosomal proteins have been shown to colocalize with VP3, and it is possible that translation also occurs within the VFs, although this remains to be experimentally verified [56]. The role of the VFs in viral assembly also remains poorly understood.…”

Section: Ibdv Vf Processesmentioning

confidence: 97%

“…In this assay, 137 interacting proteins were identified, giving an indication as to the breadth of possible VP3 clients that may be recruited to VFs during infection. Protein-protein interaction (PPI) analysis of 121 of these proteins revealed several functionally connected groups, for example numerous mutually-interacting ribosomal subunit proteins, clusters of metabolically active proteins, regulatory proteins of the actin cytoskeleton, and cardiomyocyte adrenergic signaling molecules, indicating the breadth of possible functions of the VP3-scaffolded VF interaction network [56]. However, while defining the VP3 interactome by Co-IP and mass-spectrometry provides valuable information for follow-up studies, the necessary cell lysis steps in the protocol means any LLPS structures would be lost, and different approaches are required for defining the IBDV VF composition.…”

Section: Ibdv Vf Compositionmentioning

confidence: 99%

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The Formation and Function of Birnaviridae Virus Factories

Brodrick

Broadbent

2023

IJMS

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The use of infectious bursal disease virus (IBDV) reverse genetics to engineer tagged reporter viruses has revealed that the virus factories (VFs) of the Birnaviridae family are biomolecular condensates that show properties consistent with liquid–liquid phase separation (LLPS). Although the VFs are not bound by membranes, it is currently thought that viral protein 3 (VP3) initially nucleates the formation of the VF on the cytoplasmic leaflet of early endosomal membranes, and likely drives LLPS. In addition to VP3, IBDV VFs contain VP1 (the viral polymerase) and the dsRNA genome, and they are the sites of de novo viral RNA synthesis. Cellular proteins are also recruited to the VFs, which are likely to provide an optimal environment for viral replication; the VFs grow due to the synthesis of the viral components, the recruitment of other proteins, and the coalescence of multiple VFs in the cytoplasm. Here, we review what is currently known about the formation, properties, composition, and processes of these structures. Many open questions remain regarding the biophysical nature of the VFs, as well as the roles they play in replication, translation, virion assembly, viral genome partitioning, and in modulating cellular processes.

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Section: Ibdv Vf Processesmentioning

confidence: 97%

Section: Ibdv Vf Compositionmentioning

confidence: 99%

The Formation and Function of Birnaviridae Virus Factories

Brodrick

Broadbent

2023

IJMS

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“…Similarly, the 3′-extremity region of rabbit hemorrhagic disease virus (RHDV) interacts with RPS5 to promote replication of viral genes ( Guo et al, 2020 ). Infectious bursal disease virus (IBDV) VP3 protein interacts with RPs to form ribosomal protein assembly complexes to promote viral genome replication ( Ma et al, 2021 ). HIV-1 encodes Gag proteins that interact with RPL7 to facilitate the assembly of viral particles ( Karnib et al, 2020 ).…”

Section: Ribosomal Proteins Exert Non-ribosomal Functions During Rna ...mentioning

confidence: 99%

“…However, the specific functions need further study. Similarly, a proteomic approach has been used to explore the interactions between the encoded viral proteins and host proteins following IBDV infection ( Ma et al, 2021 ). Therefore, the above finding demonstrates the feasibility of this approach for determining the role of viral proteins following viral invasion of the host.…”

Section: Ribosomal Proteins Exert Non-ribosomal Functions During Rna ...mentioning

confidence: 99%

Ribosomal control in RNA virus-infected cells

et al. 2022

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Viruses are strictly intracellular parasites requiring host cellular functions to complete their reproduction cycle involving virus infection of host cell, viral genome replication, viral protein translation, and virion release. Ribosomes are protein synthesis factories in cells, and viruses need to manipulate ribosomes to complete their protein synthesis. Viruses use translation initiation factors through their own RNA structures or cap structures, thereby inducing ribosomes to synthesize viral proteins. Viruses also affect ribosome production and the assembly of mature ribosomes, and regulate the recognition of mRNA by ribosomes, thereby promoting viral protein synthesis and inhibiting the synthesis of host antiviral immune proteins. Here, we review the remarkable mechanisms used by RNA viruses to regulate ribosomes, in particular, the mechanisms by which RNA viruses induce the formation of specific heterogeneous ribosomes required for viral protein translation. This review provides valuable insights into the control of viral infection and diseases from the perspective of viral protein synthesis.

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