Effect of load-induced local mechanical strain on peri-implant bone cell activity related to bone resorption and formation in mice: An analysis of histology and strain distributions

Okawara, Hisami; Arai, Yuki; Matsuno, Hitomi; Marcián, Petr; Borák, Libor; Aoki, Kazuhiro; Wakabayashi, Noriyuki

doi:10.1016/j.jmbbm.2021.104370

Cited by 13 publications

(6 citation statements)

References 46 publications

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“…Interestingly, one study correlating finite elements investigations for continuously loaded implants with the biological response in the mice model demonstrated wider calcified distance in the area with highest strain intensity ( 14 ). They therefore suggested that bone formation activity, particularly the calcification ability of osteoblasts, was locally activated in the highest strain area, which was also reflected in the present histological analysis.…”

Section: Discussionmentioning

confidence: 99%

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Bone remodelling patterns around orthodontic mini-implants migrating in bone: an experimental study in rat vertebrae

Becker

Rauch

Brunello

et al. 2021

European Journal of Orthodontics

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Summary Background Orthodontic implant migration has been clinically observed in presence of continuous loading forces. Recent studies indicate that osteocytes play a crucial role in this phenomenon. Objectives Aim of this study was to investigate local osteocytic gene expression, protein expression, and bone micro-structure in peri-implant regions of pressure and tension. Material and methods The present work reports a complementary analysis to a previous micro-computed tomography study. Two customized mini-implants were placed in one caudal rat vertebra and connected by a nickel–titanium contraction spring generating different forces (i.e. 0, 0.5, 1.0, and 1.5 N). Either at 2 or 8 weeks, the vertebrae were harvested and utilized for 1. osteocytic gene expression using laser capture micro-dissection on frozen sections coupled with qPCR, 2. haematoxylin–eosin staining for qualitative and quantitative analyses, 3. immunofluorescence staining and analysis, and 4. bone-to-implant contact on undecalcified samples. Results At the two time points for all the performed analyses no significant differences were observed with respect to the applied force magnitudes and cell harvesting localization. However, descriptive histological analysis revealed remarkable bone remodelling at 2 weeks of loading. At 8 weeks the implants were osseointegrated and, especially in 1.0 and 1.5 N groups, newly formed bone presented a characteristic load bearing architecture with trabecula oriented in the direction of the loading. Conclusions The present study confirmed that stress-induced bone remodelling is the biological mechanism of orthodontic implant migration. Bone apposition was found at ‘tension’ and ‘pressure’ sites thus limiting implant migration over time.

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Section: Discussionmentioning

confidence: 99%

“…Analysis of load-driven mechanotransduction has emerged as a fundamental step to elucidate the impact of orthodontic loads on peri-implant tissues ( 13 , 14 ). Osteocytes are recognized to be the principal sensory cells responding to mechanical stimuli that alter the interstitial fluid flow ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

Bone remodelling patterns around orthodontic mini-implants migrating in bone: an experimental study in rat vertebrae

Becker

Rauch

Brunello

et al. 2021

European Journal of Orthodontics

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“…From the different signaling pathways of the osteocytes, their mechanosensitivity is determinant to ensure bone mechanical integrity (Cowin et al, 1991;Delgado-Calle and Bellido, 2022;Palumbo and Ferretti, 2021). It is believed that abnormal mechanical stimulation explains the complex peri-implant bone organization (Gramanzini et al, 2016;Li et al, 2018;Okawara et al, 2021).…”

Section: What Is Bone Mechanical Integrity?mentioning

confidence: 99%

Osteocyte pericellular and perilacunar matrices as markers of bone–implant mechanical integrity

Gauthier¹,

Follet²,

Trunfio-Sfarghiu³

et al. 2022

Biocell

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To develop durable bone healing strategies through improved control of bone repair, it is of critical importance to understand the mechanisms of bone mechanical integrity when in contact with biomaterials and implants. Bone mechanical integrity is defined here as the adaptation of structural properties of remodeled bone in regard to an applied mechanical loading. Accordingly, the authors present why future investigations in bone repair and regeneration should emphasize on the matrix surrounding the osteocytes. Osteocytes are mechanosensitive cells considered as the orchestrators of bone remodeling, which is the biological process involved in bone homeostasis. These bone cells are trapped in an interconnected porous network, the lacunocanalicular network, which is embedded in a bone mineralized extracellular matrix. As a consequence of an applied mechanical loading, the bone deformation results in the deformation of this lacunocanalicular network inducing a shift in interstitial fluid pressure and velocity, thus resulting in osteocyte stimulation.The material environment surrounding each osteocyte, the so called perilacunar and pericellular matrices properties, define its mechanosensitivity. While this mechanical stimulation pathway is well known, the laws used to predict bone remodeling are based on strains developing at a tissue scale, suggesting that these strains are related to the shift in fluid pressure and velocity at the lacunocanalicular scale. While this relationship has been validated through observation in healthy bone, the fluid behavior at the bone-implant interface is more complex. The presence of the implant modifies fluid behavior, so that for the same strain at a tissue scale, the shift in fluid pressure and velocity will be different than in a healthy bone tissue. In that context, new markers for bone mechanical integrity, considering fluid behavior, have to be defined. The viewpoint exposed by the authors indicates that the properties of the pericellular and the perilacunar matrices have to be systematically investigated and used as structural markers of fluid behavior in the course of bone biomaterial development.

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“…This occurs because the response of bone cells, speci cally in terms of bone formation and resorption, is in uenced by local mechanical strains that occur during implant loading. (3,4) Wider implant distribution without a framework cantilever is likely to result in better biomechanical behavior and less technical complications in prosthetic components. (5,6) Due to limited availability of viable osseous tissue, bone density and proximity to vital structures, dental implants are commonly presented to the prosthodontist where cantilevers are unavoidable.…”

Section: Introductionmentioning

confidence: 99%

Biomechanical behavior of implant retained prostheses in the posterior maxilla using different materials: A finite element study

Aboelfadl,

Keilig,

Ebeid

et al. 2024

Preprint

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Background: In this study, the biomechanical behavior of the mesial and distal off-axial extensions of implant-retained prostheses in the posterior maxilla with different prosthetic materials were investigated using FEA. Methods: 3D finite element models with three implant prosthetic designs: fixed-fixed, mesial cantilever, and distal cantilever were designed depending upon cone beam CT images of an intact maxilla of an anonymous patient. The 3D modeling software Mimics Innovation Suite (Mimics 14.0 / 3-matic 7.01; Materialise, Leuven, Belgium) was used. All the models were imported into the FE package Marc/Mentat (ver. 2015; MSC Software, Los Angeles, Calif). The material properties of the implant, bone, monolithic zirconia, and polyetherketoneketone polymer (PEKK) were taken from literature. Then, individual models were subjected to separate axial loads of 300 N. Stress values were computed for the prosthesis, bone, and implant under axial loading. Results: Distal off-axial extension designs generated the maximum von Mises stresses (105 MPa) while the fixed-fixed designs generated the minimum von Mises stresses (35 MPa). The zirconia distal cantilever model yielded the most elevated levels of von Mises stresses within the prosthesis (105 MPa), with concentrated patterns at the implant prosthesisinterface near the offset extension. The von Mises stresses were highest in the implant (112 MPa) and compact bone (100 MPa) for the PEKK distal cantilever model. On the other hand, the zirconia fixed-fixed model exhibited the minimum von Mises stresses in the implant (49 MPa) and compact bone (20 MPa). Conclusions: The fixed-fixed prosthetic design showed the lowest von Mises stresses. In clinically challenging conditions Mesial cantilever design together with a rigid prosthetic material is suggested as a second alternative with acceptable biomechanical behavior.

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Effect of load-induced local mechanical strain on peri-implant bone cell activity related to bone resorption and formation in mice: An analysis of histology and strain distributions

Cited by 13 publications

References 46 publications

Bone remodelling patterns around orthodontic mini-implants migrating in bone: an experimental study in rat vertebrae

Bone remodelling patterns around orthodontic mini-implants migrating in bone: an experimental study in rat vertebrae

Osteocyte pericellular and perilacunar matrices as markers of bone–implant mechanical integrity

Biomechanical behavior of implant retained prostheses in the posterior maxilla using different materials: A finite element study

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