New sample preparation method to analyse 15 specific and non-specific pesticide metabolites in human urine using LC-MS/MS

Fišerová, Petra Stuchlík; Kohoutek, Jiří; Degrendele, Céline; Dalvie, Mohamed Aqiel; Klánová, Jana

doi:10.1016/j.jchromb.2021.122542

Cited by 11 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Urine plastic containers were given to each child and collected after they were filled with urine, according to the sampling protocol. These were subsequently collected into 8-mL plastic vials and kept in a freezer until they were shipped to the accredited trace analytical laboratories, as described by Fišerová et al 54 Urine samples were sent to RECETOX, Masaryk University, Czech Republic, for further analysis.…”

Section: Methodsmentioning

confidence: 99%

Child exposure to organophosphate and pyrethroid insecticides measured in urine, wristbands, and household dust and its implications for child health in South Africa: A panel study

Veludo,

Röösli,

Dalvie

et al. 2023

Environmental Epidemiology

Self Cite

View full text Add to dashboard Cite

Background: Children in agricultural areas are exposed to organophosphate (OP) and pyrethroid (PYR) insecticides. This explorative study investigated child exposure to OPs and PYRs, comparing temporal and spatial exposure variability within and among urine, wristbands, and dust samples. Methods: During spraying season 2018, 38 South African children in two agricultural areas (Grabouw/Hex River Valley) and settings (farm/village) participated in a seven-day study. Child urine and household dust samples were collected on days 1 and 7. Children and their guardians were wearing silicone wristbands for seven days. Intraclass correlation coefficients (ICCs) evaluated temporal agreements between repeated urine and dust samples, Spearman rank correlations (Rs) evaluated the correlations among matrices, and linear mixed-effect models investigated spatial exposure predictors. A risk assessment was performed using reverse dosimetry. Results: Eighteen OPs/PYRs were targeted in urine, wristbands, and dust. Levels of chlorpyrifos in dust (ICC = 0.92) and diethylphosphate biomarker in urine (ICC = 0.42) showed strong and moderate temporal agreement between day 1 and day 7, respectively. Weak agreements were observed for all others. There was mostly a weak correlation among the three matrices (Rs = −0.12 to 0.35), except for chlorpyrifos in dust and its biomarker 3,5,6-trichloro-2-pyridinol in urine (Rs = 0.44). No differences in exposure levels between living locations were observed. However, 21% of the urine biomarker levels exceeded the health-risk threshold for OP exposure. Conclusions: Observed high short-term variability in exposure levels during spraying season highlights the need for repeated sampling. The weak correlation between the exposure matrices points to different environmental and behavioral exposure pathways. Exceeding risk thresholds for OP should be further investigated.

show abstract

Section: Methodsmentioning

confidence: 99%

Child exposure to organophosphate and pyrethroid insecticides measured in urine, wristbands, and household dust and its implications for child health in South Africa: A panel study

Veludo,

Röösli,

Dalvie

et al. 2023

Environmental Epidemiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…A multiple reaction monitoring (MRM) method was used for quantification of 4-OH-BF. Data were acquired and processed using the MassLynx 4.1 software …”

Section: Materials and Methodsmentioning

confidence: 99%

Stage Dependent Enantioselective Metabolism of Bifenthrin in Embryos of Zebrafish (Danio rerio) and Japanese Medaka (Oryzias latipes)

Tanabe

Shi

et al. 2021

Environ. Sci. Technol.

View full text Add to dashboard Cite

Bifenthrin (BF) is a widely used pyrethroid that has been frequently detected in surface waters. Previous studies indicated that BF had antiestrogenic activity in zebrafish embryos but estrogenic activity in posthatch fish. To determine whether age-related differences in metabolism contribute to the endocrine effects in developing fish, embryos from zebrafish and Japanese medaka were exposed to BF before and after liver development. Since the commercial mixture of BF is an isomer-enriched product containing two enantiomers (1R-cis-BF and 1S-cis-BF), enantioselective metabolism was also evaluated. The estrogenic metabolite, 4-hydroxybifenthrin (4-OH-BF) was identified in zebrafish embryos, and formation was higher in animals after liver development (>48 hpf). Treatments with β-glucuronidase indicated that 4-OH-BF underwent conjugation in embryos. Formation was reduced by cotreatment of the cytochrome P450 (CYP450) inhibitor, ketoconazole. Formation of 4-OH-BF was greater when treated with 1R-cis-BF compared to the S-enantiomer. However, metabolites were not observed in medaka embryos. These data indicate enantioselective oxidation of BF to an estrogenic metabolite occurs in zebrafish embryos and, since it is increased after liver development, may partially explain estrogenic activity observed in older animals. The lack of activity in medaka suggests species-specific effects with BF metabolism and may influence risk assessment strategies in wildlife.

show abstract

“…Consequently, many concerns have arisen worldwide about the negative impacts of pesticides on the environment and human health. In particular, agricultural region residents are a highly exposed population, with higher quantification frequencies and levels of pesticides found in various environmental media from rural areas, such as air [ 15 , 16 ], dust [ 17 , 18 , 19 ], silicone wristbands [ 20 , 21 ], and human samples such as urine [ 22 , 23 , 24 ] and blood [ 25 , 26 ]. In addition, several epidemiological studies have found an association between residential proximity to agricultural fields and diseases such as autism [ 27 , 28 ], Parkinson’s disease [ 29 ], childhood cancer [ 30 , 31 ], and also with neurobehavioral effects [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Human Exposure to Pesticides in Dust from Two Agricultural Sites in South Africa

Degrendele

Prokeš

Šenk

et al. 2022

Toxics

View full text Add to dashboard Cite

Over the last decades, concern has arisen worldwide about the negative impacts of pesticides on the environment and human health. Exposure via dust ingestion is important for many chemicals but poorly characterized for pesticides, particularly in Africa. We investigated the spatial and temporal variations of 30 pesticides in dust and estimated the human exposure via dust ingestion, which was compared to inhalation and soil ingestion. Indoor dust samples were collected from thirty-eight households and two schools located in two agricultural regions in South Africa and were analyzed using high-performance liquid chromatography coupled to tandem mass spectrometry. We found 10 pesticides in dust, with chlorpyrifos, terbuthylazine, carbaryl, diazinon, carbendazim, and tebuconazole quantified in >50% of the samples. Over seven days, no significant temporal variations in the dust levels of individual pesticides were found. Significant spatial variations were observed for some pesticides, highlighting the importance of proximity to agricultural fields or of indoor pesticide use. For five out of the nineteen pesticides quantified in dust, air, or soil (i.e., carbendazim, chlorpyrifos, diazinon, diuron and propiconazole), human intake via dust ingestion was important (>10%) compared to inhalation or soil ingestion. Dust ingestion should therefore be considered in future human exposure assessment to pesticides.

show abstract

New sample preparation method to analyse 15 specific and non-specific pesticide metabolites in human urine using LC-MS/MS

Cited by 11 publications

References 34 publications

Child exposure to organophosphate and pyrethroid insecticides measured in urine, wristbands, and household dust and its implications for child health in South Africa: A panel study

Child exposure to organophosphate and pyrethroid insecticides measured in urine, wristbands, and household dust and its implications for child health in South Africa: A panel study

Stage Dependent Enantioselective Metabolism of Bifenthrin in Embryos of Zebrafish (Danio rerio) and Japanese Medaka (Oryzias latipes)

Human Exposure to Pesticides in Dust from Two Agricultural Sites in South Africa

Contact Info

Product

Resources

About