Polyaniline@Au organic-inorganic nanohybrids with thermometer readout for photothermal immunoassay of tumor marker

Zhang, Bing; Hu, Xing; Jia, Yejing; Li, Jing; Zhang, Zhao

doi:10.1007/s00604-021-04719-y

Cited by 19 publications

(8 citation statements)

References 36 publications

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“…The plot of the time value and −ln (θ) was displayed in Figure 2E, and its slope was 99.526. Using Eqs 6, 10, the η of AuNC@Fe 3 O 4 was calculated to be 39.58%, which was similar with or higher than the PPT reagents previously reported, such as, EA-Fe@BSA NPs (31.2%) (Tian et al, 2020), Fe 3 O 4 @ Carbon@Platinum-Chlorin e6 (28.28%) (Xu et al, 2022b), Au nanorods (22%) (Zeng et al, 2013), Au nanoshells (13%) (Hessel et al, 2011), PANi@Au (40.4%) and Au nanoparticles (21.7%) (Zhang et al, 2021a).…”

Section: Photothermal Performance Of Aunc@ Fe 3 Osupporting

confidence: 81%

A peroxidase-like magneto-gold nanozyme AuNC@Fe3O4 with photothermal effect for induced cell apoptosis of hepatocellular carcinoma cells in vitro

Shi

Liu

Wang

2023

Front. Bioeng. Biotechnol.

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Hepatocellular carcinoma (HCC) is one of the most commonly diagnosed and malignant cancers worldwide. Conventional therapy strategies may not completely eradicate the tumor and may cause side effects during treatment. Nano-catalytic therapy, as a novel strategy, has attracted a great deal of attention. This study aimed to synthesize a multifunctional magneto-gold nanozyme AuNC@Fe3O4 and evaluate its anti-cancer potential in HepG2 cells in vitro. The characteristics of AuNC@Fe3O4 were assessed using a transmission electron microscope, dynamic light scattering, and energy-dispersive X-ray. The photothermal performance and peroxidase (POD)-like activity of AuNC@Fe3O4 were detected, using thermal camera and ultraviolet-visible spectrophotometer, respectively. The anti-cancer potential of AuNC@Fe3O4 was examined using cell counting kit-8, live/dead cell staining, and apoptosis analysis. Further research on HepG2 cells included the detection of intracellular reactive oxygen species (ROS) and lysosomal impairment. We observed that the AuNC@Fe3O4 had a small size, good photothermal conversion efficiency and high POD-like activity, and also inhibited cell proliferation and enhanced cell apoptotic ability in HepG2 cells. Furthermore, the AuNC@Fe3O4 enhanced ROS production and lysosomal impairment via the synergistic effect of photothermal and nano-catalytic therapies, which induced cell death or apoptosis. Thus, the magneto-gold nanozyme AuNC@Fe3O4 may offer a potential anti-cancer strategy for HCC.

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Section: Photothermal Performance Of Aunc@ Fe 3 Osupporting

confidence: 81%

A peroxidase-like magneto-gold nanozyme AuNC@Fe3O4 with photothermal effect for induced cell apoptosis of hepatocellular carcinoma cells in vitro

Shi

Liu

Wang

2023

Front. Bioeng. Biotechnol.

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“…[ 73 ] Furthermore, the photothermal antibacterial is mainly to inoculate the photothermal material to the wound infected by bacteria and then use NIR light for fixed‐point irradiation, resulting in achieving the effect of treating wound bacteria infection. [ 55 ] At present, the traditional organic PTC materials, including polymer polypyrrole, [ 74 ] polyaniline, [ 75 ] and organic small molecule dyes, [ 76 ] are successfully used in the biomedical field. However, to achieve a better effect of NIR photothermal treatment, traditional organic PTC materials need to obtain substantial value in the NIR region via complicated design and tedious synthesis, which greatly limits their practical applications.…”

Section: Applications Of Organic Photothermal Cocrystalsmentioning

confidence: 99%

Organic Photothermal Cocrystals: Rational Design, Controlled Synthesis, and Advanced Application

et al. 2023

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Organic photothermal cocrystals, integrating the advantages of intrinsic organic cocrystals and the fascinating photothermal conversion ability, hold attracted considerable interest in both basic science and practical applications, involving photoacoustic imaging, seawater desalination, and photothermal therapy, and so on. However, these organic photothermal cocrystals currently suffer individual cases discovered step by step, as well as the deep and systemic investigation in the corresponding photothermal conversion mechanisms is rarely carried out, suggesting a huge challenge for their further developments. Therefore, it is urgently necessary to investigate and explore the rational design and synthesis of high‐performance organic photothermal cocrystals for future applications. This review first and systematically summarizes the organic photothermal cocrystal in terms of molecular classification, the photothermal conversion mechanism, and their corresponding applications. The timely interpretation of the cocrystal photothermal effect will provide broad prospects for the purposeful fabrication of excellent organic photothermal cocrystals toward great efficiency, low cost, and multifunctionality.

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“…Microfluidics, electrochemistry, colorimetry, and temperature probes have all been proposed to achieve this goal [8][9][10][11] . Covid-19 test kits and pregnancy test kits are well-known POC devices based on lateral flow immunoassays.…”

Section: Introductionmentioning

confidence: 99%

Building a nucleic acid nanostructure with DNA-epitope conjugates for a versatile electrochemical protein detection platform

Gurukandure

Somasundaram

Kurian

et al. 2023

Preprint

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The recent surge of effort in nucleic acid-based electrochemical (EC) sensors has been fruitful, and some have even shown real-time quantification of drugs in the blood of living animals. Yet there remains a need for more generalizable EC platforms for the detection of multiple classes of clinically relevant targets. Our group has recently reported a nucleic acid nanostructure that permits simple, economical, and generalizable EC readout of a wide range of analytes (small molecules, peptides, large proteins, or antibodies). The DNA nanostructure is built through on-electrode enzymatic ligation of three oligonucleotides for attachment, binding, and signaling. However, the signaling mechanism predominantly relies on tethered diffusion of methylene blue at the electrode surface, limiting the detection of larger proteins that have no readily available small molecule binding partners. In this study, we adapted the nanostructure sensor to quantify larger proteins in a more generic manner, through conjugating the proteins minimized antibody-binding epitope to the central DNA strand of the nanostructure (DNA-peptide conjugate). This concept was verified using creatine kinase (CK-MM), an important biomarker of muscle damage, myocardial infarction, overexertion/rhabdomyolysis, or neuromuscular disorders where clinical outcomes could be improved with rapid sensing. DNA-epitope conjugates permitted a competitive immunoassay protocol at the electrode surface for quantifying CK protein. Square-wave voltammetry (SWV) signal suppression was proportional to the amount of surface-bound antibody with a limit of detection (LOD) of 5 nM and a response time as low as 3 minutes, and displacement of antibody by native CK-MM protein analyte could also be assayed. CK was quantified from the LOD of 14 nM up to 100 nM, overlapping well with the normal (non-elevated) human clinical range of 3 37 nM, and the sensor response was validated in 98% human serum. While a need for improved DNA-epitope conjugate purification was identified, overall this approach not only allows the detection of a generic protein- or peptide-binding antibody, but it also should facilitate future quantitative EC readout of various clinically relevant protein analytes that were previously inaccessible to EC techniques.

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Polyaniline@Au organic-inorganic nanohybrids with thermometer readout for photothermal immunoassay of tumor marker

Cited by 19 publications

References 36 publications

A peroxidase-like magneto-gold nanozyme AuNC@Fe3O4 with photothermal effect for induced cell apoptosis of hepatocellular carcinoma cells in vitro

A peroxidase-like magneto-gold nanozyme AuNC@Fe3O4 with photothermal effect for induced cell apoptosis of hepatocellular carcinoma cells in vitro

Organic Photothermal Cocrystals: Rational Design, Controlled Synthesis, and Advanced Application

Building a nucleic acid nanostructure with DNA-epitope conjugates for a versatile electrochemical protein detection platform

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