Overexpression of WssgtL3.1 gene from Withania somnifera confers salt stress tolerance in Arabidopsis

Mishra, Manoj Kumar; Tiwari, Shalini; Misra, Pratibha

doi:10.1007/s00299-021-02666-9

Cited by 13 publications

(6 citation statements)

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“…Specifically, UDP-GTs contribute to bolstering resistance against these stresses by modulating the activities of glycosylated hormones and secondary metabolites within plants (O’Donnell et al 1998). In W. somnifera , increased levels of sterol glycosyltransferase expression were noted to confer resistance against diverse environmental stresses (Chaturvedi and Mishra 2012; Mishra et al 2013, 2017, 2021, 2022; Pandey et al 2014; Saema et al 2016; Singh et al 2016). In agreement with these results, leaves from the WsGT4 and WsGT6 silenced plants exhibited pronounced disease symptoms and endured greater tissue damage compared to leaves from control plants (Figure S9A).…”

Section: Discussionmentioning

confidence: 99%

“…Silencing the WsSGTL1 gene in W. somnifera resulted in increased accumulation of withanolides and phytosterols, while reducing the content of glycowithanolides, along with increased susceptibility to pathogens (Saema et al 2015;Singh et al 2016). In addition to WsSGTL1, another sterol glucosyltransferase (SGTL) named WssgtL3.1 exhibited a protective function against salt stress and biotic stress when overexpressed in transgenic Arabidopsis plants (Mishra et al 2021(Mishra et al , 2022. Similarly, the overexpression of Ws-Sgtl4 resulted in elevated total withanolide yield and increased levels of withanolide-A content in the transformed W. somnifera hairy roots (Pandey et al 2016).…”

Section: Introductionmentioning

confidence: 99%

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Functional characterization of two glycosyltransferases fromWithania somniferailluminates their role in withanosides biosynthesis and defence against bacteria

Anjali,

Narayanan,

Parihar

et al. 2024

Preprint

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The medicinal properties of Ashwagandha (Withania somniferaL. Dunal) are attributed to the presence of unique class of natural products called as withanolides and their glycosylated forms, withanosides. Withanosides are proposed to be formed from withanolides by the action of glycosyltransferases (GTs). This study reports the functional characterization of two GTs (WsGT4andWsGT6) fromW. somniferathat exhibited induced expression in response to methyl jasmonate treatment and showed highest expression in leaves compared to other tissues. Biochemical assays with recombinant WsGT proteins showed that WsGT4 and WsGT6 formed glycosylated products with four and one of the seven tested withanolides substrates, respectively. WsGT4 catalyzed product formation using withanolide A, withanolide B, withanone, and 12-deoxywithastramonolide as substrates, with UDP-glucose serving as the glucose donor, while WsGT6 catalyzed the product formation only with withaferin A as substrate employing either UDP-glucose or UDP-galactose as sugar donors. Moreover,in plantastudies through virus-induced gene silencing and transient overexpression ofWsGT4andWsGT6inW. somniferaleaves modulated the levels of withanolides and withanosides, indicating their role in withanosides biosynthesis. Furthermore, while individual silencing of bothWsGT4andWsGT6inW. somniferareduced the tolerance toPseudomonas syringaeDC3000 growth, their overexpression enhanced the tolerance to the bacterium inW. somnifera. Taken together, these results shed light on the roles of WsGT4 and WsGT6 in withanoside biosynthesis and defence against model bacterial pathogen inW. somnifera.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Functional characterization of two glycosyltransferases fromWithania somniferailluminates their role in withanosides biosynthesis and defence against bacteria

Anjali,

Narayanan,

Parihar

et al. 2024

Preprint

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“…4 B, 10 B, and 12 B). The content of sterol glycosides can be affected by various stress factors like cold, drought, and salt stress [ 35 – 37 ]; increased sterol glycosylation was observed after treatment with jasmonic acid of both hairy roots and C. officinalis plants in the previous study [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Modifications in steroid and triterpenoid metabolism in Calendula officinalis plants and hairy root culture in response to chitosan treatment

2023

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Background Chitosan, a deacetylated derivative of chitin, is one of the most preferred biopolymers for use as biostimulants and biofertilizers in organic agriculture and as elicitors to enhance the productivity of plant in vitro cultures. Valued as a non-toxic, biodegradable, and environment-friendly agent, it is widely applied to improve plant growth and yield, the content of bioactive specialized metabolites, and resistance to stress conditions and pathogens. However, the influence of chitosan on the growth-defense trade-off, particularly the interplay between steroid and triterpenoid metabolism, has not been extensively investigated. Results In this study, Calendula officinalis pot plants and hairy root cultures exposed to chitosan treatment displayed reduced biomass and altered steroid and triterpenoid metabolism. Biosynthesis and accumulation of free forms of sterols (particularly stigmasterol) were inhibited, while the content of sterol esters increased remarkably. The content of some triterpenoids (mainly free triterpenoid acids) was slightly enhanced; however, the biosynthesis of triterpenoid saponins was negatively affected. Conclusions These results indicate that in certain plants, chitosan treatment might not positively influence the growth and metabolite production. Therefore, to avoid unexpected effects, initial studies of the conditions of chitosan treatment are recommended, including the dose and the number of chitosan applications, the type of treatment (e.g., foliar or soil), and the vegetative stage of the treated plants.

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“…Higher germination reflects better growth and development of seedlings and thus leads to higher crop production [ 27 ]. Overexpression of the SsMT2 gene in transgenic yeast improved the tolerance of the cells to NaCl, CdCl 2 and H 2 O 2 [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of the GmMT-II Gene and Its Role in Response to High Temperature and Humidity Stress in Glycine max

Liu

et al. 2022

Plants

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Metallothioneins (MTs) are polypeptide-encoded genes involved in plant growth, development, seed formation, and diverse stress response. High temperature and humidity stress (HTH) reduce seed development and maturity of the field-grown soybean, which also leads to seed pre-harvest deterioration. However, the function of MTs in higher plants is still largely unknown. Herein, we isolated and characterized the soybean metallothionein II gene. The full-length fragment is 255 bp and encodes 85 amino acids and contains the HD domain and the N-terminal non-conservative region. The subcellular location of the GmMT-II-GFP fusion protein was clearly located in the nucleus, cytoplasm, and cell membrane. The highest expression of the GmMT-II gene was observed in seeds both of the soybean Xiangdou No. 3 and Ningzhen No. 1 cultivars, as compared to other plant tissues. Similarly, gene expression was higher 45 days after flowering followed by 30, 40, and 35 days. Furthermore, the GmMT-II transcript levels were significantly higher at 96 and 12 h in the cultivars Xiangdou No. 3 and Ningzhen No. 1 under HTH stress, respectively. In addition, it was found that when the Gm1-MMP protein was deleted, the GmMT-II could bind to the propeptide region of the Gm1-MMP, but not to the signal peptide region or the catalytic region. GmMT-II overexpression in transgenic Arabidopsis increased seed germination and germination rate under HTH conditions, conferring enhanced resistance to HTH stress. GmMT-II overexpressing plants suffered less oxidative damage under HTH stress, as reflected by lower MDA and H2O2 content and ROS production than WT plants. In addition, the activity of antioxidant enzymes namely SOD, CAT, and POD was significantly higher in all transgenic Arabidopsis lines under HTH stress compared wild-tpye plants. Our results suggested that GmMT-II is related to growth and development and confers enhanced HTH stress tolerance in plants by reduction of oxidative molecules through activation of antioxidant activities. These findings will be helpful for us in further understanding of the biological functions of MT-II in plants.

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Overexpression of WssgtL3.1 gene from Withania somnifera confers salt stress tolerance in Arabidopsis

Cited by 13 publications

References 50 publications

Functional characterization of two glycosyltransferases fromWithania somniferailluminates their role in withanosides biosynthesis and defence against bacteria

Functional characterization of two glycosyltransferases fromWithania somniferailluminates their role in withanosides biosynthesis and defence against bacteria

Modifications in steroid and triterpenoid metabolism in Calendula officinalis plants and hairy root culture in response to chitosan treatment

Isolation and Characterization of the GmMT-II Gene and Its Role in Response to High Temperature and Humidity Stress in Glycine max

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