High prevalence of SARS-CoV-2 infection among food delivery riders. A case study from Quito, Ecuador

Ortíz-Prado, Esteban; Henríquez-Trujillo, Aquiles R.; Rivera-Olivero, Ismar A.; Lozada, Tannya; García-Bereguiaín, Miguel Angel

doi:10.1016/j.scitotenv.2021.145225

Cited by 48 publications

(32 citation statements)

References 27 publications

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“…This is in accordance with the findings in the overall SARS-CoV-2 attack rate for males and females in our study. The overall high SARS-CoV-2 attack rate of 12.65% is also on agreement with other reports showing SARS-CoV-2 community transmission among community dwelling individuals in Ecuador (6)(7)(8)19,20).…”

Section: Discussionsupporting

confidence: 91%

COVID-19 outbreaks at shelters for women who are victims of gender-based violence from Ecuador

Vallejo-Janeta

Morales-Jadán

Freire‐Paspuel

et al. 2021

International Journal of Infectious Diseases

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Section: Discussionsupporting

confidence: 91%

COVID-19 outbreaks at shelters for women who are victims of gender-based violence from Ecuador

Vallejo-Janeta

Morales-Jadán

Freire‐Paspuel

et al. 2021

International Journal of Infectious Diseases

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“…Largely, UDLA has contributed with almost 10% of the total SARS-CoV-2 testing during the first semester of COVID-19 pandemic in Ecuador. Moreover, as our surveillance was mainly focused on asymptomatic or mild symptomatic population not attending medical facilities at neglected urban, rural and indigenous communities, the high positivity rate of 23,06% obtained at our lab supports the hypothesis that massive community transmission of SARS-CoV-2 has been happening in Ecuador [ 5 ].…”

Section: The Role Of the Universities Improving Sars-cov-2 Surveillance In Neglected Provinces And Communitiessupporting

confidence: 71%

“…1 ). Our contribution to SARS-CoV-2 surveillance was particularly relevant for the provinces of Pichincha, Manabí, Esmeraldas and Chimborazo, where more than 10% of the total SARS-CoV-2 RT-PCR tests were done at our laboratory ( Table 1 ) [ 5 , 6 ]. Moreover, 61.9% of the total SARS-CoV-2 RT-PCR tests done at the Amazonian province of Pastaza were done at our laboratory.…”

Section: The Role Of the Universities Improving Sars-cov-2 Surveillance In Neglected Provinces And Communitiesmentioning

confidence: 99%

Crucial contribution of the universities to SARS-CoV-2 surveillance in Ecuador: Lessons for developing countries

et al. 2021

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COVID-19 pandemic has challenged public health systems worldwide, particularly affecting developing countries in Latin America like Ecuador. In this report, we exposed the fundamental role of the Ecuadorian universities to improve COVID-19 surveillance in the country, with an overall contribution over 15% of the total SARS-CoV-2 RT-PCR tests done. We highlight the role of our university during the first semester of the COVID-19 pandemic, contributing to a massive free SARS-CoV-2 testing up to almost 10% of the total diagnosis completed in the country, mainly focus on underserved urban, rural and indigenous communities. Finally, we described our contribution to a high quality and low-cost SARS-CoV-2 RT-PCR diagnostic in Ecuador.

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“…All the samples included in the study were tested following an adapted version of the CDC protocol: (a) using Accupower Viral RNA extraction kit (Bioneer, South Korea) as an alternate RNA extraction method; (b) using CFX96 BioRad instrument ( Freire-Paspuel et al., 2020a ; Freire-Paspuel and Garcia-Bereguiain, 2020 ; Freire-Paspuel et al., 2020 ; Freire-Paspuel et al., 2020b ; Freire-Paspuel et al., 2020 ; Freire-Paspuel and Garcia-Bereguiain, 2021b ; Freire-Paspuel et al., 2021 ; Ortiz-Prado et al., 2021 ); (c) using a N1/N2/RNaseP multiplex assay ( Freire-Paspuel and Garcia-Bereguiain, 2021a ). The criteria for positivity was a Ct ≤ 40 for N1 and N2 targets simultaneously ( CDC, 2020 ; Xiaoyan et al., 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Analytical and Clinical Evaluation of “AccuPower SARS-CoV-2 Multiplex RT-PCR kit (Bioneer, South Korea)” and “Allplex 2019-nCoV Assay (Seegene, South Korea)” for SARS-CoV-2 RT-PCR Diagnosis: Korean CDC EUA as a Quality Control Proxy for Developing Countries

Freire‐Paspuel

García-Bereguiaín

2021

Front. Cell. Infect. Microbiol.

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BackgroundMultiple RT-qPCR kits are available in the market for SARS-CoV-2 diagnosis, some of them with Emergency Use Authorization (EUA) by FDA or their country of origin agency, but many of them lack of proper clinical evaluation.ObjectiveWe evaluated the clinical performance of two Korean SARS-CoV-2 RT-PCR kits available in South America, AccuPower SARS-CoV-2 Multiplex RT-PCR kit (Bioneer, South Korea) and Allplex 2019-nCoV Assay (Seegene, South Korea), for RT-qPCR SARS-CoV-2 diagnosis using the CDC protocol as a gold standard.ResultsWe found strong differences among both kits clinical performance and analytical sensitivity; while the Allplex 2019-nCoV Assay has sensitivity of 96.5% and an estimated limit of detection of 4,000 copies/ml, the AccuPower SARS-CoV-2 Multiplex RT-PCR kit has a sensitivity of 75.5% and limit of detection estimated to be bigger than 20,000 copies/ml.ConclusionsAccuPower SARS-CoV-2 Multiplex RT-PCR kit and Allplex 2019-nCoV Assay are both made in South Korea but EUA by Korean CDC was only granted to the later. Our results support that Korean CDC EUA should be considered as a quality control proxy for Korean SARS-CoV-2 RT-PCR kits prior to importation by developing countries to guarantee high sensitivity diagnosis.

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High prevalence of SARS-CoV-2 infection among food delivery riders. A case study from Quito, Ecuador

Cited by 48 publications

References 27 publications

COVID-19 outbreaks at shelters for women who are victims of gender-based violence from Ecuador

COVID-19 outbreaks at shelters for women who are victims of gender-based violence from Ecuador

Crucial contribution of the universities to SARS-CoV-2 surveillance in Ecuador: Lessons for developing countries

Analytical and Clinical Evaluation of “AccuPower SARS-CoV-2 Multiplex RT-PCR kit (Bioneer, South Korea)” and “Allplex 2019-nCoV Assay (Seegene, South Korea)” for SARS-CoV-2 RT-PCR Diagnosis: Korean CDC EUA as a Quality Control Proxy for Developing Countries

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