In vivo demonstration of Congo Red labeled amyloid plaques via perfusion in the Alzheimer disease rat model

Setti, Sharay E.; Raymick, James; Hanig, Joseph P.; Sarkar, Sumit

doi:10.1016/j.jneumeth.2021.109082

Cited by 10 publications

(4 citation statements)

References 23 publications

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“…Khalil et. and Setti et al declared that compromised insulin signalling causes amyloid β peptide accumulation in the brain [ 25 , 46 ]. Interestingly, treatment with either liraglutide or pramlintide in our study showed a reduction in Aβ plaque burden in the hippocampus with Congo red stain and attenuation of p-tau217 expression with immunohistochemical examination as compared to the diabetic group.…”

Section: Discussionmentioning

confidence: 99%

Liraglutide versus pramlintide in protecting against cognitive function impairment through affecting PI3K/AKT/GSK-3β/TTBK1 pathway and decreasing Tau hyperphosphorylation in high-fat diet- streptozocin rat model

Moghazy,

Abdelhaliem,

Mohammed

et al. 2024

Pflugers Arch - Eur J Physiol

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The American Diabetes Association guidelines (2021) confirmed the importance of raising public awareness of diabetes-induced cognitive impairment, highlighting the links between poor glycemic control and cognitive impairment. The characteristic brain lesions of cognitive dysfunction are neurofibrillary tangles (NFT) and senile plaques formed of amyloid-β deposition, glycogen synthase kinase 3 beta (GSK3β), and highly homologous kinase tau tubulin kinase 1 (TTBK1) can phosphorylate Tau proteins at different sites, overexpression of these enzymes produces extensive phosphorylation of Tau proteins making them insoluble and enhance NFT formation, which impairs cognitive functions. The current study aimed to investigate the potential contribution of liraglutide and pramlintide in the prevention of diabetes-induced cognitive dysfunction and their effect on the PI3K/AKT/GSK-3β/TTBK pathway in type 2 diabetic (T2D) rat model. T2D was induced by administration of a high-fat diet for 10 weeks, then injection of a single dose of streptozotocin (STZ); treatment was started with either pramlintide (200 μg/kg/day sc) or liraglutide (0.6 mg/kg/day sc) for 6 weeks in addition to the HFD. At the end of the study, cognitive functions were assessed by novel object recognition and T-maze tests. Then, rats were sacrificed for biochemical and histological assessment of the hippocampal tissue. Both pramlintide and liraglutide treatment revealed equally adequate control of diabetes, prevented the decline in memory function, and increased PI3K/AKT expression while decreasing GSK-3β/TTBK expression; however, liraglutide significantly decreased the number of Tau positive cells better than pramlintide did. This study confirmed that pramlintide and liraglutide are promising antidiabetic medications that could prevent associated cognitive disorders in different mechanisms.

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Section: Discussionmentioning

confidence: 99%

Liraglutide versus pramlintide in protecting against cognitive function impairment through affecting PI3K/AKT/GSK-3β/TTBK1 pathway and decreasing Tau hyperphosphorylation in high-fat diet- streptozocin rat model

Moghazy,

Abdelhaliem,

Mohammed

et al. 2024

Pflugers Arch - Eur J Physiol

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“…The dye binds to the β-sheet structure found in amyloid fibrils and detects a significant change in the highest absorbance from 490 to 540 nm and green birefringence with blended polarized light [78,79]. It has been demonstrated that CR detects amyloid fibrils in brain tissue [80]. Furthermore, in algal adhesive, the green-gold birefringence was observed after staining with CR indicating amyloid formation (Figure 3c).…”

Section: Characterization Of Amyloid Fibril Formationmentioning

confidence: 95%

Amyloidogenesis: What Do We Know So Far?

Alraawi

Banerjee

Mohanty

et al. 2022

IJMS

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The study of protein aggregation, and amyloidosis in particular, has gained considerable interest in recent times. Several neurodegenerative diseases, such as Alzheimer’s (AD) and Parkinson’s (PD) show a characteristic buildup of proteinaceous aggregates in several organs, especially the brain. Despite the enormous upsurge in research articles in this arena, it would not be incorrect to say that we still lack a crystal-clear idea surrounding these notorious aggregates. In this review, we attempt to present a holistic picture on protein aggregation and amyloids in particular. Using a chronological order of discoveries, we present the case of amyloids right from the onset of their discovery, various biophysical techniques, including analysis of the structure, the mechanisms and kinetics of the formation of amyloids. We have discussed important questions on whether aggregation and amyloidosis are restricted to a subset of specific proteins or more broadly influenced by the biophysiochemical and cellular environment. The therapeutic strategies and the significant failure rate of drugs in clinical trials pertaining to these neurodegenerative diseases have been also discussed at length. At a time when the COVID-19 pandemic has hit the globe hard, the review also discusses the plausibility of the far-reaching consequences posed by the virus, such as triggering early onset of amyloidosis. Finally, the application(s) of amyloids as useful biomaterials has also been discussed briefly in this review.

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“…Another important factor that adds and confirms the value of the results obtained with bikaverin is related to the high toxicity of the Congo red pigment. Using such a harmful substance makes BC unfriendly to the environment, from contaminating soil, riverbeds, and lakes (Alsamhary, K., et al 2022) to causing problems in various tissues of the human body (Frid, P., Anisimov, S. V., & Popovic, N. 2007), mainly in the central nervous system (Setti, S. E., et al 2021). In terms of energy production optimization, the mediators proved to be quite efficient in raising a maximum blank Id, from 155 mA.cm -2 to 230 mA.cm -2 with bikaverin and 273 mA.cm -2 , an increase of 150% and 176% respectively.…”

Section: Energy Profile Of Electrochemical Mediation In a Bcmentioning

confidence: 99%

Microbial fuel cell (MFC) performance with pigments bikaverin and congo red as electrochemical mediators for optmization power energy

Silva

Nascimento

Campos-Takaki

2022

RSD

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For decades, non-renewable energy resources have been used indiscriminately, but their slow depletion and extremely harmful impacts on the environment have shifted the focus to sustainable and renewable energy sources. Among the renewable energy sources, biofuel cells are defined as devices that convert chemical energy present in chemical bonds into electrical energy. Biocells are classified into two broad categories of enzymatic fuel cells, which employ enzymes as biocatalysts, and microbial fuel cells, which use microorganisms as biocatalysts. An important requirement in the functioning of a biofuel cell is the transfer of electrons from inside an active site of an enzyme to the outside, as the electrodes being solid cannot penetrate the enzymes. A wide range of molecules can be used as electrochemical mediators, some with high toxicity and many non-toxic fungal substances having an enormous potential to be used as electrochemical mediators. In this work, the fungal pigment bikaverin was compared to the synthetic dye Congo red, in order to obtain the best energy-optimizing molecule in an enzymatic fuel cell. Congo red presented a higher current density of 273 mA.cm-2 compared to bikaverin, 230 mA.cm-2, but because it presents a more stable chronoamperometric graph and does not have high toxicity, the fungal biopigment proved to be the best option for optimization. on the potential of energy generated in an enzymatic fuel cell.

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In vivo demonstration of Congo Red labeled amyloid plaques via perfusion in the Alzheimer disease rat model

Cited by 10 publications

References 23 publications

Liraglutide versus pramlintide in protecting against cognitive function impairment through affecting PI3K/AKT/GSK-3β/TTBK1 pathway and decreasing Tau hyperphosphorylation in high-fat diet- streptozocin rat model

Liraglutide versus pramlintide in protecting against cognitive function impairment through affecting PI3K/AKT/GSK-3β/TTBK1 pathway and decreasing Tau hyperphosphorylation in high-fat diet- streptozocin rat model

Amyloidogenesis: What Do We Know So Far?

Microbial fuel cell (MFC) performance with pigments bikaverin and congo red as electrochemical mediators for optmization power energy

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