This study established a database on non-pathological gait kinematics (treadmill gait and overground gait) and conducted statistical analysis, using markerless motion capture technology (MMC method) verified through system introduction and verification. Data were collected in three ways, and the collected gait images were analyzed using the MMC method gait analysis system. The mean and standard deviations of gait parameters, such as cadence, shoulder and pelvic horizontal angle, and flexion/extension motions at shoulder, hip, knee, and ankle joints, were evaluated to be utilized as nonpathologic gait norm data of the markerless method. As a result of statistical analysis, the gait speed in non-pathological treadmill walking was found to have a significant effect on some gait kinematics, such as shoulder horizontal angle, shoulder joint angle, hip joint angle, and knee angle. And the gait type (treadmill gait and over ground gait) also affected some of the gait kinematics. As a case study, pathologic over-ground gait was compared with non-pathological gait, and differences were found in the shoulder and pelvis horizontal angle patterns and the timing of maximum flexion/extension of the shoulder joint and lower extremity joint. This database of non-pathologic gait would be valuable for the comparative assessment of pathological gaits, which was one of the significant purposes of general application to the biomedical and healthcare industries.