Pellicle formation by Escherichia coli K-12: Role of adhesins and motility

Abstract

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Polymer-induced biofilms for enhanced biocatalysis

“…Interestingly, when we evaluated how this improvement in MC4100 pSTB7's ability to carry out this biotransformation correlated with some of the phenotypes previously investigated, we observed that this increase in performance was better correlated to the ability of these polymers to increase the amount of biomass and promote aggregation in MC4100 pSTB7 (Figure 5A,B) but not to curli expression (Figure 5C). This correlation is in line with our observation that this adhesin is overexpressed in floating but not attached biofilms, 49 and that biofilms are a suitable platform for this biotransformation. [42][43][44] Similarly, there was a very poor correlation to the degree of functionalization (Figure S58) or to the effect these polymers have on the metabolic activity of…”

“…This observation is in agreement with some recent results that show that curli expression in E. coli K-12 strains is upregulated in planktonic cells and floating biofilms, also known as pellicles, but gets downregulated once bacteria settle on solid surfaces. 49 When we compared curli expression for MC4100 in the presence of these polymers to that of PHL644, we could see that, like in the case of biomass, MC4100 was now reaching levels of GFP fluorescence similar to, if not higher than, those for PHL644 (Figure 3B). This increase in fluorescence was particularly the case for P1-mod-2AFPA, P1-mod-OctA and P1-mod-9AntA, the best performing polymers in this assay.…”

Polymer-Induced Biofilms for Enhanced Biocatalysis

“…Interestingly, when we evaluated how this improvement in MC4100 pSTB7's ability to carry out this biotransformation correlated with some of the phenotypes previously investigated, we observed that this increase in performance was better correlated to the ability of these polymers to increase the amount of biomass and promote aggregation in MC4100 pSTB7 (Figure 5A,B) but not to curli expression (Figure 5C). This correlation is in line with our observation that this adhesin is overexpressed in floating but not attached biofilms, 46 and that biofilms are a suitable platform for this biotransformation. [39][40][41] Similarly, there was a very poor correlation to the effect these polymers have on the metabolic activity of MC4100 (Figure 5D).…”

Capture and Ex-Situ Analysis of Environmental Biofilms in Livestock Buildings

“…Microscopic visualization of extracellular matrix compounds may be considered in future experiments. Alternative labeling methods can be used in future experiments to distinguish other components of the biofilm, such as fluorescent lectins that bind specifically to exopolysaccharides, thioflavin T for amyloid fiber labeling, or fluorescence in situ hybridization (FISH) technique by detecting specific species having access to their spatial organization [45,46].…”