Arabidopsis TCX8 functions as a senescence modulator by regulating LOX2 expression

Noh, Minsoo; Shin, Jong Son; Hong, Jong Chan; Kim, Soo Youn; Shin, Jeong Sheop

doi:10.1007/s00299-021-02663-y

Cited by 6 publications

(4 citation statements)

References 46 publications

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“…It will thus be interesting to see in future if such a phosphorylation impacts DREAM complex assembly and if so, which kinase is involved. Furthermore, to our knowledge, only TCX proteins with the ALM motif have been found to interact with other MuvB-core proteins in planta ( Kobayashi et al, 2015 ; Ning et al, 2020 ), although recently TCX8 has been found to bind to ALY3 in the Y2H system ( Noh et al, 2021 ). So either the non-ALM motif-bearing LIN54 homologs of Arabidopsis are part of less stable or less abundant versions of the DREAM complex, making complex precipitation from plant material difficult or they do not build a DREAM complex at all but rather function as transcriptional regulators in different assemblies.…”

Section: Discussionmentioning

confidence: 92%

“…In addition to the previously described DREAM components these complexes also included the RBBP4 homolog MSI1, two LIN37 homologs as well as two uncharacterized proteins termed DREAM COMPONENT 1 (DRC1) and DREAM COMPONENT 2 (DRC2) ( Ning et al, 2020 ). In addition, different plant homologs of LIN54, namely, TSO1, TCX2 (SOL2), TCX3 (SOL1), and TCX8, have been shown to be involved in different developmental processes, for example, in reproductive development ( Liu et al, 1997 ; Hauser et al, 2000 ; Song et al, 2000 ; Andersen et al, 2007 ; Sijacic et al, 2011 ), the formation of stomata ( Simmons et al, 2019 ) and tracheary elements ( Clark et al, 2019 ), as well as senescence ( Noh et al, 2021 ). However, the formation of a DREAM complex remained hypothetical in these contexts.…”

Section: Introductionmentioning

confidence: 99%

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The DREAM complex represses growth in response to DNA damage in Arabidopsis

et al. 2021

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The DNA of all organisms is constantly damaged by physiological processes and environmental conditions. Upon persistent damage, plant growth and cell proliferation are reduced. Based on previous findings that RBR1, the only Arabidopsis homolog of the mammalian tumor suppressor gene retinoblastoma, plays a key role in the DNA damage response in plants, we unravel here the network of RBR1 interactors under DNA stress conditions. This led to the identification of homologs of every DREAM component in Arabidopsis, including previously not recognized homologs of LIN52. Interestingly, we also discovered NAC044, a mediator of DNA damage response in plants and close homolog of the major DNA damage regulator SOG1, to directly interact with RBR1 and the DREAM component LIN37B. Consistently, not only mutants in NAC044 but also the double mutant of the two LIN37 homologs and mutants for the DREAM component E2FB showed reduced sensitivities to DNA-damaging conditions. Our work indicates the existence of multiple DREAM complexes that work in conjunction with NAC044 to mediate growth arrest after DNA damage.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

The DREAM complex represses growth in response to DNA damage in Arabidopsis

et al. 2021

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“…This could be realised through incorporation of different component homologues with separate sets of target genes, e.g. whereas TCX5 and TCX6 promote flowering, TCX8 is able to bind to ALY3 and represses LIPOXYGENASE 2 (LOX2) expression (Noh et al, 2021). Interestingly, it has been demonstrated that LOX2 is up-regulated upon floral transition, and there are hints at a delayed-flowering phenotype in the corresponding mutants (Bañuelos et al, 2008;Wang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

The two plant-specific DREAM components FLIC and FLAC repress floral transition inArabidopsis

Lang,

Böwer,

Elbaşı

et al. 2023

Preprint

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The DREAM complex is a key transcriptional regulator especially involved in the control of the cell cycle and development. Here, we characterise two novel plant- specific DREAM components, FLIC and FLAC, which we identified through tandem affinity purification experiments as interactors of conserved core DREAM constituents. We demonstrate that plants lacking both FLIC and FLAC exhibit pleiotropic phenotypes, including stunted growth and reduced fertility. Notably,flic flacdouble mutants show an early-flowering phenotype, an aspect that we found to be shared with mutants of the core DREAM component LIN37, with which FLIC and FLAC interact in binary protein-protein interaction assays. Performing reverse affinity purification experiments, we detected the JMJ14/NAC050/NAC052 module, known for its involvement in flowering repression, in the interactome of both FLIC and FLAC. Subsequent binary interaction studies then link the JMJ14/NAC050/NAC052 module via LIN37 to the DREAM complex providing a mechanistic framework on how flowering time could be transcriptionally controlled by the DREAM complex.Summary blurbThis study identifies two plant-specific members of the DREAM complex, explores their roles by mutant analysis and protein interaction investigation, and links them and additional DREAM complex components to the regulation of floral transition.

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“…AtTCP15 and related proteins mediate the development of the gynoecium through their involvement in auxin and cytokinin responses [45]. Noh et al provided evidence that AtTCP20 and TCX8 delay plant senescence through their participation in jasmonic acid biosynthesis [46]. Previous studies reported that plants adapt to salt stress by regulating the dynamic changes in hormone biosynthesis [41] (e.g., ABA plays an essential position in salt stress signal transduction of B. platyphylla [47] and Populus euphratica [5]).…”

Section: Discussionmentioning

confidence: 99%

BpTCP3 Transcription Factor Improves Salt Tolerance of Betula platyphylla by Reducing Reactive Oxygen Species Damage

Ren

Jiang

et al. 2021

Forests

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The plant-specific transcription factors TEOSINTE BRANCHED1/CYCLO IDEA/PROLIFERATING CELL FACTOR1 (TCP) act as developmental regulators that have many roles in the growth and development processes throughout the entire life span of plants. TCP transcription factors are responsive to endogenous and environmental signals, such as salt stress. However, studies on the role of the TCP genes in salt stress response have rarely focused on woody plants, especially forest trees. In this study, the BpTCP3 gene, a CYC/TB1 subfamily member, isolated from Betula platyphylla Sukaczev, was significantly influenced by salt stress. The β-glucuronidase (GUS) staining analysis of transgenic B. platyphylla harboring the BpTCP3 promoter fused to the reporter gene GUS (pBpTCP3::GUS) further confirmed that the BpTCP3 gene acts a positive regulatory position in salt stress. Under salt stress, we found that the BpTCP3 overexpressed lines had increased relative/absolute high growth but decreased salt damage index, hydrogen peroxide (H2O2), and malondialdehyde (MDA) levels versus wild-type (WT) plants. Conversely, the BpTCP3 suppressed lines exhibited sensitivity to salt stress. These results indicate that the BpTCP3 transcription factor improves the salt tolerance of B. platyphylla by reducing reactive oxygen species damage, which provides useful clues for the functions of the CYC/TB1 subfamily gene in the salt stress response of B. platyphylla.

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Arabidopsis TCX8 functions as a senescence modulator by regulating LOX2 expression

Cited by 6 publications

References 46 publications

The DREAM complex represses growth in response to DNA damage in Arabidopsis

The DREAM complex represses growth in response to DNA damage in Arabidopsis

The two plant-specific DREAM components FLIC and FLAC repress floral transition inArabidopsis

BpTCP3 Transcription Factor Improves Salt Tolerance of Betula platyphylla by Reducing Reactive Oxygen Species Damage

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