Quantitative evaluation of Danqi tablet by ultra‐performance liquid chromatography coupled with triple quadrupole mass spectrometry integrated with bioassay

Abstract

Danqi tablet composed of the dried roots of Salvia miltiorrhiza and Panax notoginseng is a well‐known Chinese patent medicine commonly used for the treatment of cardio‐cerebrovascular diseases such as coronary heart disease and myocardial ischemia. Numerous chemical constituents belonging to S. miltiorrhiza and P. notoginseng were detectable in Danqi tablet. Here, we established and validated a rapid and sensitive ultra‐performance liquid chromatography coupled with triple quadrupole mass spectrometry method for simultaneous quantification of 23 components in Danqi tablet and then successfully applied to assay 12 batches of samples from ten manufacturers. Our results demonstrated that the contents of 23 components in 12 batches of Danqi tablets varied significantly and their quality indeed existed differently based on the principal component analysis. According to the quantitative data and the loading plot of principal component analysis, five abundant compounds in Danqi tablet were selected as characteristic chemical markers possibly responsible for the quality assessment. Among them, salvianolic acid B and ginsenoside Rg1 were further chosen to be combined at 2:5 ratio to evaluate the anti‐thrombotic activity on phenylhydrazine‐induced zebrafish heart thrombosis model. Expectedly, this component combination increased the heart red blood cells intensity compared with the model group and the median effective concentration was 123.4 µg/mL, suggestive of its well anti‑thrombotic effect. This study contributed to the quantitative evaluation of Danqi tablet and indicated the combination of salvianolic acid B and ginsenoside Rg1 may be capable of reflecting the effect of Danqi tablet, thereby providing a reference for further investigations on the improvement of quality control and clinical application of Danqi tablet.

Both Methanol and Water Mixture Extracts of Panax Notoginseng Flower Affect Platelet Function and Thrombus Growth by Down-Regulating PI3K/AKT and MAPKs Signaling Pathways

“…Although the antithrombotic bene ts of P.notoginseng roots have been well documented before (Fu et al, 2021), few studies have evaluated the medicinal use of PNF for the prevention and treatment of cardiovascular diseases. Our previous studies showed that two saponin monomers (Rb2 and Rd2) in PNF signi cantly attenuated platelet function (Zuo et al, 2021).…”

Simultaneous determination of eight pyrrolizidine alkaloids in various parts of Eupatorium lindleyanum by ultra high performance liquid chromatography tandem mass spectrometry and risk assessments based on a real‐life exposure scenario

“…As two groups of diastereoisomers, compounds 1−4 and 5−8 have the same plane structure and almost the same mass spectrum features, making their separation difficult [21, 22]. To overcome this barrier, UHPLC‐MS/MS has been used for improving sensitivity and separation efficiency, while reducing acquisition time [23, 24]. Moreover, in light of the health risks associated with PAs, the recommended daily intake of PAs has been studied recently.…”