Biomolecular assembly on interdigitated electrode nanosensor for selective detection of insulin-like growth factor-1

Abstract

This research is focussed to quantify IGF1 by electroanalytical analysis on InterDigitated electrode surface and characterized by the microscopic observations. For the detection, antibody and aptamer were used to analyze the level of IGF1. The sandwich pattern (aptamer-IGF1-antibody) was designed on the chemically modified IDE surface and reached the limit of detection to 10 fM with 100 folds enhancement in the sensitivity. Different control experiments (absence of IGF1, binding with IGF2 and with non-complementary aptamer) were failed to show the current changes, discriminated the specific detection. A good detection strategy is to complement the currently following imaging systems for AAA.

High‐sensitivity immunoassay on interdigitated electrode to detect osteoporosis biological marker

“…In general, probe immobilization on the sensor surface plays a major role in target-analyte interaction. 39,40 A higher number of probe attachments enhances the binding of the analyte-target and lowers the detection limit of the target. Here, to improve antibody immobilization, two various nanomaterials such as SiNP and GNP were used.…”

“…This way of antibody immobilization improves the number of antibody attachments on the sensing surface and enhances analytical performance. In general, probe immobilization on the sensor surface plays a major role in target–analyte interaction 39,40 . A higher number of probe attachments enhances the binding of the analyte–target and lowers the detection limit of the target.…”