Diterpenoid anthraquinones as chemopreventive agents altered microRNA and transcriptome expressions in cancer cells

Su, Ying; Kuo, Min Zhan; Kuo, Yi Ting; Huang, Shao-Lun; Lee, Chia-Jung; Su, Zheng‐Yuan; Ni, Yu; Li, Dian Kun; Wu, Tien Yuan

doi:10.1016/j.biopha.2021.111260

Cited by 10 publications

(5 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…26 Compared with normal colonic mucosa, miR-200a-5p was significantly up-regulated in colorectal cancer, but colon adenoma is not substantially different from normal mucosa. 27,28 It was reported that miR-200a-5p and its target gene RNF11 were differentially expressed in cardiac tissue, and overexpressed miR-200a-5p induces RIP3-dependent necroptosis. 29 In our study, the expression of hsa-miR-500a-3p, hsa-miR -16-5p, and hsa-miR-148a-3p significantly affected the OS outcomes.…”

Section: Discussionmentioning

confidence: 99%

A Novel Defined Necroptosis-Related miRNAs Signature for Predicting the Prognosis of Colon Cancer

Yang¹,

Lü²,

Wang³

et al. 2022

IJGM

View full text Add to dashboard Cite

This study aims at exploring the relationship between necroptosis-related miRNAs and colon cancer prognosis. Methods: We downloaded the miRNA sequencing data from the TCGA, and eight differentially expressed necroptosis-related miRNAs were screened. Then, we used Cox regression analysis to establish a prediction model of necroptosis-related miRNA. Finally, the prognosis related miRNAs were used to predict the target genes, and functional analysis was used to explore the potential mechanism of these target genes. Results:The miRNA-seq data of 444 COAD cases were downloaded from TCGA. We identified 8 differentially expressed miRNAs (has-miR-16-5p, has-miR-141-3p, has-miR -148a-3p, has-miR-425-5p, has-miR-7-5p, has-miR-223-3p, has-miR-200a-5p, and has-miR -500a-3p), then Cox analysis was performed for determining eight-miRNA signature prognostic biomarkers with obviously different OS. The area under the curve (AUC) of receiver operating characteristic (ROC) curve for predicting 1-, 3-, and 5-year survival were 0.663, 0.653 and 0.639, respectively. The multivariate analysis also implied that the risk score was an independent prognostic factor considering other confounding factors (HR = 1.847, 95% CI = 1.197-2.848, P = 0.006). According to the Kaplan-Meier analysis, the expression of hsa-miR-500a-3p (P = 0.003), hsa-miR-16-5p (P = 0.004) and hsa-miR-148a-3p (P = 0.035) significantly affected OS outcomes. We predicted the target genes of these three miRNAs and then screened 10 hub genes (CCND1, SMAD3, SMAD2, CDK1, TGFB2, CDC25A, CHEK1, VEGFA, CCNE1, WEE1). In addition, CHEK1 was associated with the survival prognosis. Conclusion: Our study demonstrated that necroptosis is closely associated with colon cancer, and the model of eight necroptosis-related miRNAs are potentially useful prognostic biomarkers and therapeutic targets for colon cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

A Novel Defined Necroptosis-Related miRNAs Signature for Predicting the Prognosis of Colon Cancer

Yang¹,

Lü²,

Wang³

et al. 2022

IJGM

View full text Add to dashboard Cite

show abstract

“…DNA methylation and histone H3 methylation also reduce miR-200 expression [ 36 – 41 ]; thus, inhibitors of DNA methylation or histone methylation should increase miR-200 expression. It has also been shown that compounds such as cryptotanshinone, phthalates, dihydrotanshinone, alkylphenols, and retinoic acid affect miR-200 levels [ 42 – 44 ].…”

Section: Introductionmentioning

confidence: 99%

The miR-200 family in normal mammary gland development

Roth

Moorehead

2021

BMC Dev Biol

View full text Add to dashboard Cite

The miR-200 family of microRNAs plays a significant role in inhibiting mammary tumor growth and progression, and its members are being investigated as therapeutic targets. Additionally, if future studies can prove that miR-200s prevent mammary tumor initiation, the microRNA family could also offer a preventative strategy. Before utilizing miR-200s in a therapeutic setting, understanding how they regulate normal mammary development is necessary. No studies investigating the role of miR-200s in embryonic ductal development could be found, and only two studies examined the impact of miR-200s on pubertal ductal morphogenesis. These studies showed that miR-200s are expressed at low levels in virgin mammary glands, and elevated expression of miR-200s have the potential to impair ductal morphogenesis. In contrast to virgin mammary glands, miR-200s are expressed at high levels in mammary glands during late pregnancy and lactation. miR-200s are also found in the milk of several mammalian species, including humans. However, the relevance of miR-200s in milk remains unclear. The increase in miR-200 expression in late pregnancy and lactation suggests a role for miR-200s in the development of alveoli and/or regulating milk production. Therefore, studies investigating the consequence of miR-200 overexpression or knockdown are needed to identify the function of miR-200s in alveolar development and lactation.

show abstract

“…A DT-rich fraction was isolated from S. miltiorrhiza and analyzed through HPLC to reveal the presence of tanshinone IIA, tanshinone I, cryptotanshinone, and dihydrotanshinone (Figures and ). All of these are anthraquinone derivatives.…”

Section: Discussionmentioning

confidence: 99%

Diterpenoid Tanshinones Can Inhibit Lung Cancer Progression by Improving the Tumor Microenvironment and Downregulation of NF-κB Expression

Xu,

Guo,

Zhu

et al. 2024

ACS Omega

View full text Add to dashboard Cite

Diterpenoid tanshinones (DTs) are a bioactive fraction extracted from Salvia miltiorrhiza. High-performance liquid chromatography analysis revealed the presence of four compounds, namely, tanshinone IIA, tanshinone I, cryptotanshinone, and dihydrotanshinone. In this study, we aimed to propose a possible mechanism for the anti-lung cancer effect of DT. To do so, we utilized a lung cancer nude mice model and a lung cancer cell line (PC9) to investigate the effect of DT on lung cancer. We employed immunohistochemistry, enzyme-linked immunosorbent assay, hematoxylin and eosin staining, and immunofluorescence to analyze the pharmacological role of DT in the inhibition of lung cancer growth. The results showed that DT inhibited tumor growth, induced apoptosis in the nude mice model, and reduced inflammatory cell infiltration. Additionally, DT inhibited PC9 lung cancer cells, growth, proliferation, and migration. The mechanism of action of DT involves not only directly inhibiting cell proliferation and migration but also improving the tumor microenvironment. DT significantly increased the expression of important intestinal gap junction proteins, such as zonula occludens 1 (ZO-1) and occludin I. This upregulation contributes to the reinforcement of the intestinal mucosal barrier, thereby reducing the paracellular transport of lipopolysaccharides (LPS) through the intestine. Consequently, the decreased LPS levels lead to the inhibition of NF-κB expression and downregulation of macrophage polarization, as indicated by the decreased expression of CD68. In conclusion, this study has confirmed that DT has anti-lung cancer properties by improving the inflammatory tumor microenvironment via regulating macrophage polarization and inhibiting LPS-associated immune response. These results provide new insights into the mechanism of DT action against lung cancer.

show abstract

Diterpenoid anthraquinones as chemopreventive agents altered microRNA and transcriptome expressions in cancer cells

Cited by 10 publications

References 25 publications

A Novel Defined Necroptosis-Related miRNAs Signature for Predicting the Prognosis of Colon Cancer

A Novel Defined Necroptosis-Related miRNAs Signature for Predicting the Prognosis of Colon Cancer

The miR-200 family in normal mammary gland development

Diterpenoid Tanshinones Can Inhibit Lung Cancer Progression by Improving the Tumor Microenvironment and Downregulation of NF-κB Expression

Contact Info

Product

Resources

About