Effect of injectable platelet-rich fibrin (i-PRF) on the rate of tooth movement:

Abstract

Objectives To evaluate the efficiency of injectable platelet-rich fibrin (i-PRF) in accelerating canine tooth movement and to examine levels of the matrix metalloproteinase-8 (MMP-8), interleukin-1β (IL-1β), receptor activator of nuclear factor kappa-light-chain-enhancer of activated B cells ligand (RANKL), and osteoprotegerin (OPG) in the gingival crevicular fluid during orthodontic treatment. Materials and Methods Twenty patients (mean age = 21.4 ± 2.9 years) with Class II Division 1 malocclusion were included in a split-mouth study. The treatment plan for all patients was extraction of maxillary first premolars followed by canine distalization with closed-coil springs using 150 g of force on each side. The study group received i-PRF two times, with a 2-week interval, on one side of the maxilla. The contralateral side served as the control and did not receive i-PRF. Maxillary canine tooth movement was measured at five time points (T1–T5) on each side. Also, the activity of inflammatory cytokines was evaluated at three time points in the gingival crevicular fluid samples. Results There was a significant difference in canine tooth movement between the two groups (P < .001). i-PRF significantly increased the rate of tooth movement, and stimulation in the levels of inflammatory cytokines supported this result (P < .001). The levels of cytokines changed in both groups between T1 and T2. The IL-1β, MMP8, and RANKL values were significantly increased in the study group compared with the control group, while the OPG values were significantly decreased. Conclusions i-PRF-facilitated orthodontics is an effective and safe treatment modality to accelerate tooth movement, and this method can help shorten orthodontic treatment duration.

Inflammasomes in Alveolar Bone Loss

“…IL-1β is frequently detected in the GCF during OTM, and its production can be upregulated by increasing the orthodontic force ( 287 – 289 ). The expression of IL-1β and RANKL was found to be increased in patients undergoing orthodontic treatment using injectable platelet-rich fibrin, whereas that of OPG was found to be significantly decreased, indicating the promotion of osteoclastogenesis ( 191 ). The levels of NLRP3, caspase-1, and IL-1β were shown to be increased in the periodontium tissues of rats subjected to excessive orthodontic force, and activation of the NLRP3/caspase-1/IL-1β axis as well as polarization of M1-like macrophages was also detected in THP-1 cells when cocultured with force-pretreated hPDLCs but inhibited by the NLRP3 inhibitor MCC950 ( 156 ).…”

Platelet-rich derivatives for accelerating the rate of orthodontic tooth movement - a systematic review and meta-analysis

“…[21] A total of 133 patients were enrolled in split-mouth trials and 40 patients were enrolled in the RCT by Karakasli et al Out of the included studies, four were carried out over 12 weeks. [21][22][23][24] Two studies were carried out over a period of 5 months. [25,26] e studies by El-Timamy et al and Tehranchi et al were carried out over 4 months.…”

“…[27,28] e study by Nemtoi et al was carried out for 6 months. [16] Four out of the nine studies included iPRF, [21,23,24,26] two studies used iPRP, [22,27] and the remaining used LPRF membrane and PRF plugs as intervention. [16,25,28] In the studies using i-PRP, the administration was done at 0, 3, and 6 weeks by El-Timamy et al [27] and only once before retraction by Ali et al, [22] Erdur et al, and Karakasli et al administered PRF twice immediately after extraction of premolar teeth and during the 2 nd week of distalization.…”

“…[16,25,28] In the studies using i-PRP, the administration was done at 0, 3, and 6 weeks by El-Timamy et al [27] and only once before retraction by Ali et al, [22] Erdur et al, and Karakasli et al administered PRF twice immediately after extraction of premolar teeth and during the 2 nd week of distalization. [21,23] Zeitounlouian et al administered i-PRF twice, just before refraction and 4 weeks later. [26] Karci et al administered i-PRF only once before canine distalization [24] and in the studies by [16,25,28] All included studies reported on the amount of canine distal movement except the study by Karakasli et al, in which incisor distal movement was measured.…”

Letter to the Editor

“…1. In another study 1 published by the same authors, the site and technique of i-PRF (injectable plateletrich fibrin) was discussed. However, the present study does not give details of the injection at the four incisors.…”