Peptide-Based Photocathodic Biosensors: Integrating a Recognition Peptide with an Antifouling Peptide

Abstract

Accurate and sensitive detection of targets in practical biological matrixes such as blood, plasma, serum, or tissue fluid is a frontier issue for most biosensors since the coexistence of both potential reducing agents and protein molecules has the possibility of causing signal interference. Herein, aiming at detection in a complex environment, an advanced and robust peptide-based photocathodic biosensor, which integrated a recognition peptide with an antifouling peptide in one probe electrode, was first proposed. Selecting human chorionic gonadotropin (hCG) as a model target, the recognition peptide with the sequence PPLRINRHILTR was first anchored on the CuBi2O4/Au (CBO/Au) photocathode and then the antifouling peptide with the sequence EKEKEKEPPPPC was further anchored to generate an antifouling biointerface. The peptide-based photocathodic biosensor demonstrated excellent anti-interference to both nonspecific proteins and reducing agents because of the capability of the antifouling peptide. It also exhibited good sensitivity owing to the utilization of the recognition peptide rather than an antibody probe. This peptide-integrated method offers a new perspective for practical applications of photocathodic biosensors.

Anti-Fouling Magnetic Beads Combined with Signal Amplification Strategies for Ultra-Sensitive and Selective Electrochemiluminescence Detection of MicroRNAs in Complex Biological Media

“…Due to the strong hydration capacity of the peptide, it can resist nonspecific adsorption in complex biological environments. By adjusting the type and quantity of amino acids to make the peptide neutral, the nonspecific adsorption of proteins due to electrostatic attraction can be avoided. , In recent years, our group has developed a series of quantitative biosensors with anti-fouling properties based on peptides for detecting biomolecules in real samples. − …”

Peptide-Derived Biosensors and Their Applications in Tumor Immunology-Related Detection

“…The antifouling performance of the peptide is attributed to its hydrophilicity, neutral charge, and stable conformation, which may be used for reference in sample detection. 32 In addition, peptides can coassemble with other nanoparticles or polymers to increase the specific surface area and antifouling properties of the biosensor, thereby increasing the accumulation of signal transmission units. Xu et al built an immunoassay platform for carcinoembryonic-antigen (CEA) detection.…”

“…The biosensor had a sensitivity from 0.5 mIU mL –1 to1000 mIU mL –1 with a LOD of 0.19 mIU mL –1 , exhibiting promising feasibility for quantitative analysis. The antifouling performance of the peptide is attributed to its hydrophilicity, neutral charge, and stable conformation, which may be used for reference in real sample detection . In addition, peptides can coassemble with other nanoparticles or polymers to increase the specific surface area and antifouling properties of the biosensor, thereby increasing the accumulation of signal transmission units.…”

Argentivorous Molecules with Oxyethylene Chains in Side-Arms: Silver Ion-Induced Selectivity Changes toward Alkali Metal Ions

“…The coordination bonds are relatively strong, and the metal type and oxidation number determine the coordination angle. Also, because they share functions with bio-containing molecules such as porphyrins, peptides, and biopolyamines, − it is expected that molecules and supramolecular compounds with bio-like functions will be developed.…”