Propolis ethanolic extract has double-face in vitro effect on the planktonic growth and biofilm formation of some commercial probiotics

Alfarrayeh, Ibrahim; Fekete, Csaba; Gazdag, Zoltán; Papp, Gábor

doi:10.1016/j.sjbs.2020.11.047

Cited by 14 publications

(14 citation statements)

References 47 publications

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“…Probiotics delivered as a biofilm, i.e., an adhered or aggregated community of bacteria that produce a self-forming protective matrix of DNA, proteins, lipids, and oligosaccharides, are more resistant to harsh environmental conditions such as acidic gastric pH, laminar/turbulent fluid forces, anti-microbial agents, and host immune defenses compared to free-living planktonic bacteria [ 42 , 43 ]. The use of probiotics in their biofilm state has been investigated and utilized in a few conditions, including antagonizing pathogenic infections in implants and incorporation into anti-neoplastic strategies as immunoregulators [ 44 , 45 , 46 , 47 ]. However, the delivery of probiotics in a biofilm state is a new and innovative strategy in the management and prevention of NEC.…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus reuteri in Its Biofilm State Improves Protection from Experimental Necrotizing Enterocolitis

et al. 2021

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Necrotizing enterocolitis (NEC) is a devastating disease predominately found in premature infants that is associated with significant morbidity and mortality. Despite decades of research, medical management with broad spectrum antibiotics and bowel rest has remained relatively unchanged, with no significant improvement in patient outcomes. The etiology of NEC is multi-factorial; however, gastrointestinal dysbiosis plays a prominent role in a neonate’s vulnerability to and development of NEC. Probiotics have recently emerged as a new avenue for NEC therapy. However, current delivery methods are associated with potential limitations, including the need for at least daily administration in order to obtain any improvement in outcomes. We present a novel formulation of enterally delivered probiotics that addresses the current limitations. A single enteral dose of Lactobacillus reuteri delivered in a biofilm formulation increases probiotic survival in acidic gastric conditions, increases probiotic adherence to gastrointestinal epithelial cells, and reduces the incidence, severity, and neurocognitive sequelae of NEC in experimental models.

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Section: Introductionmentioning

confidence: 99%

Lactobacillus reuteri in Its Biofilm State Improves Protection from Experimental Necrotizing Enterocolitis

et al. 2021

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“…Every lactobacilli showed more than 90% of auto-aggregation after eight hours [14]. After bacteria attach, their autoaggregation and swarming motility accelerate and more planktonic cells present increase colony formation [18]. These activities, during the biofilm generation process, induce the maturation of biofilm [18].…”

Section: Biofilmmentioning

confidence: 99%

“…After bacteria attach, their autoaggregation and swarming motility accelerate and more planktonic cells present increase colony formation [18]. These activities, during the biofilm generation process, induce the maturation of biofilm [18]. Because probiotics can form biofilm, probiotics can initiate effective colonization and successfully treat dysbiosis, this fortifies probiotics to survive many different environmental conditions.…”

Section: Biofilmmentioning

confidence: 99%

“…Because probiotics can form biofilm, probiotics can initiate effective colonization and successfully treat dysbiosis, this fortifies probiotics to survive many different environmental conditions. Probiotics, as a consequence, can cause colonization and increased survivability of its population and species [18]. Probiotics were found by Schwendicke et al to decrease growth and biofilm formation of cariogenic-like bacteria, which includes Streptococcus mutans [19].…”

Section: Biofilmmentioning

confidence: 99%

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Quantifying the antimicrobial activity of CRISPR-Cas9-accA modified ΔB. subtilismutants againstV. harveyiandE. Coli

Hillman¹

2021

Preprint

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Probiotics are increasingly popular, currently. Probiotics have been described with the ability to treat many disorders of the gastrointestinal tract (GIT) such as irritable bowel syndrome (IBS)and Crohns disease. Types of probiotics include bacterial strains from Lactobacillus and Bifidobacterium. Probiotics can restore balance to gut microbiota by outcompeting pathogenic bacteria for nutrients and secrete antimicrobials to eliminate these bacterial pathogens. However, the viability of most advertised probiotics lose their potency due to being freeze dried into powders during storage or for consuming. Many probiotics become ineffective and produce lower CFUs while traversing through the gastric acids of the digestive system. For these reasons, this study sought to enhance the antimicrobial response of a highly potent probiotic known as Bacillus subtilis. B. subtilis has been used to treat many disorders of the gut and secrete many antimicrobials lethal for pathogenic microbes. B. subtilis was genetically modified to express CRISPR-Cas9 nuclease deletion of the accA gene B.subtilis mutants, which inhibits expression of an essential accA gene a part of the fatty acid synthesis (FAS) metabolic pathway. The CRISPR-Cas9-accA B.subtilis mutants were co-cultured with V. harveyi and E. Coli. Bacterial growth, biofilm formation, antimicrobial activity, and antibiotic resistance were quantified. It was found that B. subtilis mutants co-cultured with V. harveyi and E. Coli lessened bacterial growth, amplified biofilm with V. harveyi, reduced biofilm formation of E. Coli, the co-cultures with the mutants lacked antimicrobial activity, and increased the antibiotic resistance of V. harveyi and E. Coli. It can be concluded that there is an immense potential for using genetically engineered probiotic strains to enhance the antimicrobial activity of B. subtilis, which can amplify the reduction of pathogenic bacteria. However, the safety and frugality of using B. subtilis as a probiotic requires further consideration.

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“…Several studies have aimed to collect data concerning the antifungal properties of propolis, and the results were positive; however, differences in the antifungal properties were observed in different propolis samples because of their varying chemical composition, geographical origin, season of collection, flora, and method of extraction [ 4 , 5 ]. Propolis has antifungal activity alone [ 6 , 7 ] but also functions synergistically when used simultaneously with other antifungal agents against Trichophyton , Mycrosporum, and Candida albicans [ 8 ]. Further, a study using propolis extract showed antifungal activity towards fungi in both the planktonic form and in biofilms [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

The Scent of Antifungal Propolis

Papp

Bouchelaghem

Szekeres

et al. 2021

Sensors

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Propolis contains many effective antifungal compounds that have not yet been identified and evaluated. In addition, distinguishing samples of propolis with high antifungal activity from less active ones would be beneficial for effective therapy. Propolis samples were collected from four different geographical regions in Hungary and used to prepare ethanol extracts for analysis. First, an antifungal susceptibility test was performed on Candida albicans. Then, gas chromatography-mass spectrometry (GC-MS) and an opto-electronic nose were applied for the classification of propolis samples. In three propolis samples, the IC50 was measured between 72 and 134 µg/mL, but it was not calculable in the fourth sample. GC-MS analysis of the four propolis samples identified several compounds belonging to the various chemical classes. In the antifungal samples, the relative concentration of 11,14-eicosadienoic acid was the highest. Based on the opto-electronic electronic nose measurements, 98.4% of the original grouped antifungal/non-antifungal cases were classified correctly. We identified several molecules from propolis with potential antifungal properties. In addition, this is the first report to demonstrate the usefulness of a portable opto-electronic nose to identify propolis samples with high antifungal activity. These results may contribute to the rapid and efficient selection of new fungicide-candidate molecules and effective propolis samples for treatment.

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Propolis ethanolic extract has double-face in vitro effect on the planktonic growth and biofilm formation of some commercial probiotics

Cited by 14 publications

References 47 publications

Lactobacillus reuteri in Its Biofilm State Improves Protection from Experimental Necrotizing Enterocolitis

Lactobacillus reuteri in Its Biofilm State Improves Protection from Experimental Necrotizing Enterocolitis

Quantifying the antimicrobial activity of CRISPR-Cas9-accA modified ΔB. subtilismutants againstV. harveyiandE. Coli

The Scent of Antifungal Propolis

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