“…From this figure, it is shown that the fibrous structure prevents changes in cell shape, cells attach all over the mat and remain spherical as found in literature [11]. This is claimed to preserve the native phenotype by keeping the same cell morphology as in original tissues [12][13][14].…”
This paper describes the development of our work on electrospinning of chitosan (CS) to get pure chitosan nanofiber mats proposed as support for chondrocyte culture. Electrospinning is carried out in presence of PEO as aid, and dissolution of CS and PEO in formic acid/water mixture. The fibers were recovered using different structured collectors to evidence their role on the mat structure and advantage for cell development. Rotary cylinder, at different rotation rates, allows to align the fibers and increases their crystallinity and anisotropic mechanical performances. Square-patterned collector gives a more porous material with more cells attached. The cells fixed on nanofibers have their normal round-type morphology, contrary to the elongated structure on films or culture dish. This indicates a preservation of their original phenotype. At end, dispersion of short fibers with adhered cells is proposed for injection to repair cartilage avoiding chirurgical implantation.
“…From this figure, it is shown that the fibrous structure prevents changes in cell shape, cells attach all over the mat and remain spherical as found in literature [11]. This is claimed to preserve the native phenotype by keeping the same cell morphology as in original tissues [12][13][14].…”
This paper describes the development of our work on electrospinning of chitosan (CS) to get pure chitosan nanofiber mats proposed as support for chondrocyte culture. Electrospinning is carried out in presence of PEO as aid, and dissolution of CS and PEO in formic acid/water mixture. The fibers were recovered using different structured collectors to evidence their role on the mat structure and advantage for cell development. Rotary cylinder, at different rotation rates, allows to align the fibers and increases their crystallinity and anisotropic mechanical performances. Square-patterned collector gives a more porous material with more cells attached. The cells fixed on nanofibers have their normal round-type morphology, contrary to the elongated structure on films or culture dish. This indicates a preservation of their original phenotype. At end, dispersion of short fibers with adhered cells is proposed for injection to repair cartilage avoiding chirurgical implantation.
“…For effective contact, the adhesive must be wetted by the adherend. Wetting occurs when the bonding force at the interface between the adhesive and adherend molecules is greater than the cohesive force between the molecules of the adhesive [ 27 , 28 ]. Wettable compounds or surfaces have a contact angle <90°.…”
Wound closure is a critical step in postoperative wound recovery. Substantial advancements have been made in many different means of facilitating wound closure, including the use of tissue adhesives. Compared to conventional methods, such as suturing, tissue bioadhesives better accelerate wound closure. However, several existing tissue adhesives suffer from cytotoxicity, inadequate tissue adhesive strength, and high costs. In this study, a series of bioadhesives was produced using non-swellable spider silk-derived silk fibroin protein and an outer layer of swellable polyethylene glycol and tannic acid. The gelation time of the spider silk-derived silk fibroin protein bioadhesive is less than three minutes and thus can be used during rapid surgical wound closure. By adding polyethylene glycol (PEG) 2000 and tannic acid as co-crosslinking agents to the N-Hydroxysuccinimide (NHS), and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) reaction, the adhesive strength of the bioadhesive became 2.5 times greater than that of conventional fibrin glue adhesives. Silk fibroin bioadhesives do not show significant cytotoxicity in vitro compared with other bioadhesives. In conclusion, silk fibroin bioadhesive is promising as a new medical tool for more effective and efficient surgical wound closure, particularly in bone fractures.
“…In contrast, agglomeration of adherent chondrocytes with a round phenotype on chitosan/alginate fibers was demonstrated by Iwasaki [41]. Recent studies by Yeh et al [38] and Rogina et al [42] describe spontaneous spheroid formation on chitosan-based scaffolds for chondrocytes cultured under chondrogenic conditions. In the existing studies, parameters such as scaffold type (membranes, freeze-dried sponges, fibers, and thermogels), material topology, the nature of the neutralizing solutions, chitosan deacetylation degree (75-99%), molecular weight, combination with other materials (alginate, fibronectin, hyaluronic acid) and cell culture conditions differ.…”
The replacement of damaged or degenerated articular cartilage tissue remains a challenge, as this non-vascularized tissue has a very limited self-healing capacity. Therefore, tissue engineering (TE) of cartilage is a promising treatment option. Although significant progress has been made in recent years, there is still a lack of scaffolds that ensure the formation of functional cartilage tissue while meeting the mechanical requirements for chondrogenic TE. In this article, we report the application of flock technology, a common process in the modern textile industry, to produce flock scaffolds made of chitosan (a biodegradable and biocompatible biopolymer) for chondrogenic TE. By combining an alginate hydrogel with a chitosan flock scaffold (CFS+ALG), a fiber-reinforced hydrogel with anisotropic properties was developed to support chondrogenic differentiation of embedded human chondrocytes. Pure alginate hydrogels (ALG) and pure chitosan flock scaffolds (CFS) were studied as controls. Morphology of primary human chondrocytes analyzed by cLSM and SEM showed a round, chondrogenic phenotype in CFS+ALG and ALG after 21 days of differentiation, whereas chondrocytes on CFS formed spheroids. The compressive strength of CFS+ALG was higher than the compressive strength of ALG and CFS alone. Chondrocytes embedded in CFS+ALG showed gene expression of chondrogenic markers (COL II, COMP, ACAN), the highest collagen II/I ratio, and production of the typical extracellular matrix such as sGAG and collagen II. The combination of alginate hydrogel with chitosan flock scaffolds resulted in a scaffold with anisotropic structure, good mechanical properties, elasticity, and porosity that supported chondrogenic differentiation of inserted human chondrocytes and expression of chondrogenic markers and typical extracellular matrix.
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