XPF expression and its relationship with the risk and prognosis of colorectal cancer

Abstract

Background XPF (xeroderma pigmentosum complementation group F) is a key factor contributing to DNA damage excision of nucleotide excision repair pathway. The relationship between XPF expression and the risk and prognosis of colorectal cancer (CRC) is unclear. Methods In this experiment, a total of 824 cases of colorectal tissue were collected. XPF protein expression was detected by immunohistochemical staining. We conducted a Mann–Whitney U test in order to explore the differential expression of XPF between CRC and non-cancer controls, and the correlation between XPF expression and CRC clinicopathological parameters. Univariate and multivariate Cox regression analyses were conducted to investigate the relationship between XPF expression and CRC prognosis. The Java based software GSEA as well as STRING, David, GO, KEGG were used to explore the function and regulation network of XPF. Results The results demonstrated that the XPF expression in CRC was significantly up-regulated compared with non-tumor controls (P < 0.001) and adenoma tissue (P < 0.001). XPF protein was increased in the dynamic sequence of anal diseases to adenoma tissue to CRC. Expression of XPF was related to tumor location (P = 0.005) and tumor growth pattern (P = 0.009). The results of prognosis analysis suggested that in patients with stage T1-T2, XPF low expression may be significantly associated with better overall survival (HR = 7.978, 95% CI 1.208–52.673, P = 0.031). XPF and its interacting genes played a vital role in different processes of nucleotide excision repair pathway. XPF expression was related with Ubiquitin like protein specific protease activity. Conclusions XPF might be a promising biomarker for CRC risk, and also showed potential as a prognostic predictor in CRC patients.

Optimization of concentrations of different n-3PUFAs on antioxidant capacity in mouse hepatocytes

“…As the main member of the second defense line in the antioxidant system, GST can catalyze covalent conjugation reactions between glutathione and toxic electrophilic and hydrophobic substances to produce substances with anti-lipid peroxidation effects. In addition, it can react with some exogenous or endogenous metabolites, such as cholic acid and hormones, which are insoluble in water, to generate some water-soluble compounds, and then they are excreted from the body, thereby protecting brain tissue from antioxidant damage and reducing cellular damage by free radicals and toxic substances [ 39 ]. The results showed that appropriate concentrations of EPA, ALA, or DHA could improve GST activity, thereby reducing oxidative damage and enhancing the antioxidant capacity.…”

Perspectives of ERCC1 in early-stage and advanced cervical cancer: From experiments to clinical applications

“…The C-terminal domain of ERCC1 interacts with xeroderma pigmentosum group F (XPF), which forms a heterodimeric protein complex. The complex is considered to be the main component of the nucleotide excision repair (NER) pathway ( 15 ). There are several major pathways for repairing DNA damage in human cells, one of which is NER ( 16 ).…”