p-MEK expression predicts prognosis of patients with adenocarcinoma of esophagogastric junction (AEG) and plays a role in anti-AEG efficacy of Huaier

Li, Yuan; Mo, Shaowei; Xu, Zhiyuan; Lv, Hang; Xu, Jingli; Guo, Kaibo; Hu, Can; Wang, Xiaofeng; Chen, Guiping; Qin, Jiang‐Jiang; Cheng, Xiangdong

doi:10.1016/j.phrs.2020.105411

Cited by 13 publications

(15 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We used different solvents (etroleum ether, ethylacetate, n-butanol, ethanol, and water phases) to extract TRM, and yielded five types of extracts. Our previous studies have demonstrated that the TRMBE has the most potent anticancer activity ( Wang Y. et al, 2019 ; Yuan et al, 2021 ). Then, we applied LC-MS to analyze TRMBE and found several potential compounds ( Supplementary Figures S1–S8 ).…”

Section: Discussionmentioning

confidence: 99%

“…TRM was purchased from Bozhou Medical Materials Co., Ltd. (Anhui, China). And TRMBE was obtained as described previously ( Wang Y. et al, 2019 ; Yuan et al, 2021 ). Specifically, the fruiting bodies of TRM were powdered and reflux-extracted with heated 90% ethanol twice (2 h each time).…”

Section: Methodsmentioning

confidence: 99%

“…Fresh dilutions were made for each experiment. The concentration of TRMBE is according to previously study ( Yuan et al, 2021 ). All animal model experiments were divided mice into four groups as follows: 1) Vehicle group: normal saline containing 5% DMSO (200 µl, gavage, once a day) and normal saline (200 µl, intraperitoneal (i.p.)…”

Section: Methodsmentioning

confidence: 99%

“…It has been reported that TRM may exert anti-tumor activity through regulating immune cells, including CD4 + T cells ( Li et al, 2015 ), NK cells ( Li et al, 2015 ), macrophages ( Li et al, 2016 ), and dendritic cells (DCs) ( Pan et al, 2020 ). We have recently found that the Trametes robiniophila Murr n-butanol extract (TRMBE) comprises the major bioactive components of TRM and exhibits potent anticancer activity in gastric cancer (GC) and adenocarcinoma of the esophagogastric junction ( Wang Y. et al, 2019 ; Yuan et al, 2021 ). However, the effect of combinational use of TRMBE and other chemotherapeutic drugs is unclear.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Trametes robiniophila Murr Sensitizes Gastric Cancer Cells to 5-Fluorouracil by Modulating Tumor Microenvironment

et al. 2022

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

Trametes robiniophila Murr (TRM) is a traditional Chinese medicine which has been used in clinics for enhancing immunity and improving the efficacy of chemotherapy. However, the mechanisms of action of TRM are unknown. In the previous study, we found that the Trametes robiniophila Murr n-butanol extract (TRMBE) comprises the major bioactive components of TRM. In the present study, we aimed to assess the combinational effects of TRMBE and 5-fluorouracil (5-FU) on the treatment of gastric cancer (GC) and explore its mechanism of action. It was found that TRMBE significantly potentiated the anticancer activity of 5-FU and prolonged the survival time of mice bearing Mouse Forestomach Carcinoma (MFC) xenograft tumors. We observed that the combination of TRMBE and 5-FU decreased the risk of liver metastasis in vivo. Furthermore, the combination of TRMBE and 5-FU reduced the levels of immune cytokines IL-6, IL-10, and TGF-β and increased the level of IFN-γ in peripheral blood. This combination therapy also significantly decreased the levels of polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) and PD-1-positive CD8+ T cells and increased the levels of NK cells in tumor microenvironment (TME). However, TRMBE treatment was unable to enhance the chemosensitivity of GC to 5-FU in vivo after the depletion of CD8+ T and NK cells. Taken together, our results demonstrate that TRMBE can reshape the TME of GC by regulating PMN-MDSCs, CD8+ T cells, and NK cells, therefore improving the therapeutic effects of 5-FU. This study suggests that the combination of TRMBE and 5-FU could enhance immunity and could be a promising approach for GC treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Trametes robiniophila Murr Sensitizes Gastric Cancer Cells to 5-Fluorouracil by Modulating Tumor Microenvironment

et al. 2022

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cell counting kit-8 assay (CCK-8, Beyotime, China) was performed to detect the sensitivity of the breast cancer cell lines to the drugs ( 31 ). In short, when the cells were in good condition and growing exponentially, the culture medium was discarded and the cells were washed with PBS and incubated with trypsin for 3 minutes.…”

Section: Methodsmentioning

confidence: 99%

Identification of a DNA Methylation-Driven Genes-Based Prognostic Model and Drug Targets in Breast Cancer: In silico Screening of Therapeutic Compounds and in vitro Characterization

Tian

Zhang

et al. 2021

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

DNA methylation is a vital epigenetic change that regulates gene transcription and helps to keep the genome stable. The deregulation hallmark of human cancer is often defined by aberrant DNA methylation which is critical for tumor formation and controls the expression of several tumor-associated genes. In various cancers, methylation changes such as tumor suppressor gene hypermethylation and oncogene hypomethylation are critical in tumor occurrences, especially in breast cancer. Detecting DNA methylation-driven genes and understanding the molecular features of such genes could thus help to enhance our understanding of pathogenesis and molecular mechanisms of breast cancer, facilitating the development of precision medicine and drug discovery. In the present study, we retrospectively analyzed over one thousand breast cancer patients and established a robust prognostic signature based on DNA methylation-driven genes. Then, we calculated immune cells abundance in each patient and lower immune activity existed in high-risk patients. The expression of leukocyte antigen (HLA) family genes and immune checkpoints genes were consistent with the above results. In addition, more mutated genes were observed in the high-risk group. Furthermore, a in silico screening of druggable targets and compounds from CTRP and PRISM databases was performed, resulting in the identification of five target genes (HMMR, CCNB1, CDC25C, AURKA, and CENPE) and five agents (oligomycin A, panobinostat, (+)-JQ1, voxtalisib, and arcyriaflavin A), which might have therapeutic potential in treating high-risk breast cancer patients. Further in vitro evaluation confirmed that (+)-JQ1 had the best cancer cell selectivity and exerted its anti-breast cancer activity through CENPE. In conclusion, our study provided new insights into personalized prognostication and may inspire the integration of risk stratification and precision therapy.

show abstract

The potential therapeutic benefits of Huaier in digestive system cancer: Its chemical components, pharmacological applications and future direction

Tang,

Yi,

Yang

et al. 2024

Journal of Functional Foods

View full text Add to dashboard Cite

p-MEK expression predicts prognosis of patients with adenocarcinoma of esophagogastric junction (AEG) and plays a role in anti-AEG efficacy of Huaier

Cited by 13 publications

References 40 publications

Trametes robiniophila Murr Sensitizes Gastric Cancer Cells to 5-Fluorouracil by Modulating Tumor Microenvironment

Trametes robiniophila Murr Sensitizes Gastric Cancer Cells to 5-Fluorouracil by Modulating Tumor Microenvironment

Identification of a DNA Methylation-Driven Genes-Based Prognostic Model and Drug Targets in Breast Cancer: In silico Screening of Therapeutic Compounds and in vitro Characterization

The potential therapeutic benefits of Huaier in digestive system cancer: Its chemical components, pharmacological applications and future direction

Contact Info

Product

Resources

About