Establishment and characterization of a gill cell line from pearl gentian grouper (Epinephelus lanceolatus♂×Epinephelus fuscoguttatus♀) and its application in cadmium toxicology

Xu, Xiaohui; Song, Zhan; Li, Zan; Liu, Xiumei; Feng, Yang; Sun, Guohua; Yang, Jianmin

doi:10.1016/j.ecoenv.2020.111614

Cited by 8 publications

(4 citation statements)

References 53 publications

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“…Establishment of cell lines from G. przewalskii provides a basis for studying the environmental adaptation mechanism of fishes in the subfamily Schizothoracidae. The gill is an important organ for oxygen exchange and salinity sensing of fishes; the establishment of cell lines from gills can offer an effective tool for fish environmental adaptation mechanism, viral infection, toxicity detection and environmental monitoring (Ajitha et al ., 2021; Liu, Balmori‐Cedeno, et al ., 2020; Liu, Pham, et al ., 2020; Xu et al ., 2021). In this study, for the first time, a new cell line (GPG) was successfully established from the gill of G. przewalskii using the explant method, and it was then used for conducting preliminary toxicology studies in vitro .…”

Section: Discussionmentioning

confidence: 99%

“…Using Xfect reagent, the transfection efficiency of GPG cells was calculated to be approximately 5% (Figure 5). (Ajitha et al, 2021;Liu, Balmori-Cedeno, et al, 2020;Liu, Pham, et al, 2020;Xu et al, 2021). In this study, for the first time, a new cell line (GPG) was successfully established from the gill of G. przewalskii using the explant method, and it was then used for conducting preliminary toxicology studies in vitro.…”

Section: Transfectionmentioning

confidence: 99%

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Establishment of a cell line derived from the gills of Gymnocypris przewalskii, an endemic Schizothoracine fish from Qinghai Lake of Tibet Plateau

Wei

Liang

Yue

et al. 2022

Journal of Fish Biology

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Gymnocypris przewalskii (Naked carp), a native teleost, plays an important role in maintaining the ecological balance of Lake Qinghai (altitude, 3.2 km), the largest saline lake in China. In this study, a new gill cell line from G. przewalskii was developed using the explant technique and named as GPG. This cell line was maintained in Dulbecco's Modified Eagle Medium (DMEM) (high glucose), supplemented with 15% fetal bovine serum (FBS), and was successfully subcultured up to 32 passages. Meanwhile, this cell line was also authenticated by sequencing the mitochondrial cytochrome C oxidase subunit I (COI) and 16S rRNA genes and by chromosome analysis. With the Cytomegalovirus (CMV) promoter, the GPG cell line could express green fluorescent protein (GFP) at about 5% transfection efficiency. MTT test showed that Clostridium botulinum toxin (BTX) was toxic to the cell line. After cryopreservation with 10% dimethyl sulfoxide (DMSO), this cell line could be successfully revived at an efficiency over 70%. This study revealed that the GPG cell line could be used as materials for physio‐chemical investigation of G. przewalskii and also provided a tool for gene function study and toxicological reaction in vitro.

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Section: Discussionmentioning

confidence: 99%

Section: Transfectionmentioning

confidence: 99%

Establishment of a cell line derived from the gills of Gymnocypris przewalskii, an endemic Schizothoracine fish from Qinghai Lake of Tibet Plateau

Wei

Liang

Yue

et al. 2022

Journal of Fish Biology

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“…It can be eaten or used as an ornamental fish. Especially in the Asia-Pacific region, due to its dietary requirements, growth and production efficiency, environmental adaptability, and other qualities are keeping advantages of aquaculture [32]. China is one of the major grouper producer countries and has a huge breeding potential on a commercial scale [33].…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Dietary Zinc Sources on Growth, Element Deposition, Antioxidation, Lipid Metabolism, and Related Gene Expression in Hybrid Grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus)

Wang

Ren

Javad

et al. 2022

Aquaculture Nutrition

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This study investigated the effect of different supplements of Zn on the growth performance, antioxidant, lipid metabolism, and element deposition of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus). A total of 180 juvenile groupers (bodyweight 10.02 ± 0.01 g) were divided into three groups of 20 fish each and fed with ZnSO4, nanozinc oxide (Nano-ZnO), and dihydromyricetin-Zn (DMY-Zn) for 8 weeks(the content of Zn were 123,122.11, and 120.72 mg/kg). The findings revealed that DMY-Zn had a substantial impact on growth, feed conversion ratio, and protein efficiency ratio, whereas Nano-ZnO had a significant impact on feed conversion ratio and protein efficiency ratio. Furthermore, DMY-Zn had a positive effect on the high-density lipoprotein (HDL) content and significantly upregulated the lipid synthesis gene expression (FASN and ACACA) in the liver. The fish fed DMY-Zn had larger levels of catalase (CAT) in both the serum and liver, as well as Cu/Zn-superoxide dismutase (SOD) enzymes in muscle. In terms of antioxidant-related gene expression, the DMY-Zn treatment upregulated the expression of SOD1, Keap1b, and Mt2. Moreover, DMY-Zn also had a prominent effect on element deposition, increasing the concentration of Fe and Zn in the fish liver and muscle and Cu only in the fish muscle. The results revealed that a fish-fed diet enriched with DMY-Zn had significantly higher antioxidant and lipid metabolism activities than a ZnSO4-fed diet. In conclusion, dietary DMY-Zn treatment appears to be effective in increasing hybrid grouper growth, element deposition, antioxidant activity, and lipid metabolism.

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“…As an excellent in vitro platform, fish cell lines have been widely applied to the study of virology, immunology, toxicology, preservation of germplasm resources, gene screening and functional analysis (Collet et al., 2018; Liu et al., 2020; Ruiz‐Palacios et al., 2020). For instance, fathead minnow (FHM) cell line was applied to the propagation of multiple fish viruses, including viral haemorrhagic septicaemia virus (VHSV), nervous necrosis virus (NNV) and infectious pancreatic necrosis virus (Huang et al., 2018; Lorenzen et al., 1999; Zhang, Li, et al., 2019); Lateolabrax japonicus brain cell line, derived from the brain of sea perch, was used for studying the interaction between NNV and sea perch (Jia et al., 2016; Xiang et al., 2021); and Fundulus heteroclitus brain cell line, pearl gentian grouper gill cell line and Trachinotus ovatus kidney cell line were applied to investigate the toxicity of PS‐100, Cd, Cu and Hg, respectively (Ruiz‐Palacios et al., 2020; Xu et al., 2021; Zhou et al., 2017). Since the establishment of the first fish cell line RTG‐2 in 1962, more than 880 fish cell lines have been established to date (Robin et al., 2020; Wolf & Quimby, 1962).…”

Section: Introductionmentioning

confidence: 99%

Establishment and characterization of a liver cell line, ALL, derived from yellowfin sea bream, Acanthopagrus latus, and its application to fish virology

Li¹,

Jia

Yu³

et al. 2021

Journal of Fish Diseases

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Yellowfin sea bream (Acanthopagrus latus) is an important economic fish, which is seriously threatened by various fish viruses. In this study, a cell line designated as ALL derived from the liver of yellowfin sea bream was developed and characterized. The cell line grew well in Dulbecco's modified Eagle's medium containing 10%-20% foetal bovine serum at 28°C. Amplification of the cytochrome B gene indicated that ALL cells originated from yellowfin sea bream. The modal chromosome number of ALL cells was 48. ALL cells were efficiently transfected with pEGFP-N3 plasmids, indicating the potential application of ALL cells in exogenous gene manipulation studies. ALL cells were susceptive to three main fish viruses, including viral haemorrhagic septicaemia virus (VHSV), red-spotted grouper nervous necrosis virus (RGNNV) and largemouth bass virus (LMBV). The replication of VHSV, RGNNV and LMBV in ALL cells was confirmed by quantitative real-time polymerase chain reaction, virus titre and transmission electron microscopy assays. Moreover, ALL cells could respond to VHSV, RGNNV and LMBV infections, as indicated by the differential expression of antiviral genes involving in the innate immune response. In conclusion, the newly established ALL cell line will be an excellent in vitro platform for the study of the virus-yellowfin sea bream interaction.

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Establishment and characterization of a gill cell line from pearl gentian grouper (Epinephelus lanceolatus♂×Epinephelus fuscoguttatus♀) and its application in cadmium toxicology

Cited by 8 publications

References 53 publications

Establishment of a cell line derived from the gills of Gymnocypris przewalskii, an endemic Schizothoracine fish from Qinghai Lake of Tibet Plateau

Establishment of a cell line derived from the gills of Gymnocypris przewalskii, an endemic Schizothoracine fish from Qinghai Lake of Tibet Plateau

Effect of Different Dietary Zinc Sources on Growth, Element Deposition, Antioxidation, Lipid Metabolism, and Related Gene Expression in Hybrid Grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus)

Establishment and characterization of a liver cell line, ALL, derived from yellowfin sea bream, Acanthopagrus latus, and its application to fish virology

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