Endocrine disrupting effects of binary mixtures of 17β-estradiol and testosterone in adult female western mosquitofish (Gambusia affinis)

Gui-zhen, Fang; Huang, Guo-Yong; Ying, Guang‐Guo; Qiu, Shuqing; Shi, Wenjun; Xie, Lingtian; Yang, Yuanyuan; Ma, Dongdong

doi:10.1016/j.ecoenv.2020.111566

Cited by 16 publications

(5 citation statements)

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“…17β-estradiol (E2) is one of the three main self-producing estrogens and is considered an important hormone in women (10). E2 has a crucial influence on the growth and function of the female reproductive system, and of the mammary gland under physiological conditions (11)(12)(13). E2 is involved in the induction of malignant transformation of several types of cancer cells via regulation of the associated biological processes, such as proliferation, invasion and migration (14,15).…”

Section: Introductionmentioning

confidence: 99%

Activation of GPER by E2 promotes proliferation, invasion and migration of breast cancer cells by regulating the miR‑124/CD151 pathway

et al. 2021

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Breast cancer is one of the most common malignancies worldwide and is responsible for a high mortality rate. However, the underlying pathological mechanism of breast cancer remains unclear. MicroRNAs (miRNAs/miRs) play critical roles in the progression of breast cancer. Recent studies have reported that miR-124/CD151 participates in the development of breast cancer. However, the exact molecular mechanism of miR-124/CD151 action in 17β-estradiol (E2)-treated breast cancer cells remains unknown. Thus, the present study aimed to investigate miR-124 and CD151 expression levels in MCF-7 cells treated with E2 via reverse transcription-quantitative PCR and western blot analyses. Bioinformatic analysis was performed to predict and identify whether CD151 is a potential target of miR-124. The Cell Counting Kit-8 and colony formation assays were performed to detect proliferation of MCF-7 cells. In addition, the invasive and migratory abilities of MCF-7 cells were assessed via the Transwell and wound healing assays, respectively. The results demonstrated that E2 downregulated miR-124 expression, while upregulating G protein -coupled estrogen receptor (GPER) expression in MCF-7 cells. Following treatment with the GPER antagonist, G15, miR-124 expression was significantly enhanced and E2-induced proliferation, invasion and migration of MCF-7 cells were notably inhibited. In addition, CD151 was confirmed as a direct target of miR-124. CD151 silencing remarkably suppressed the proliferation, invasion and migration of E2-induced MCF-7 cells. Taken together, these results suggest that upregulation of GPER expression induced by E2 promotes proliferation, invasion and migration of breast cancer cells by regulating the miR-124/CD151 pathway. Thus, the results of the present study provide a potential novel method for the treatment and prognosis of breast cancer.

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Section: Introductionmentioning

confidence: 99%

Activation of GPER by E2 promotes proliferation, invasion and migration of breast cancer cells by regulating the miR‑124/CD151 pathway

et al. 2021

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“…Similar results were also found in the liver of male medaka that were treated with antiandrogens vinclozolin and flutamide [ 79 ]. The steroidogenic acute regulatory protein ( star ) is involved in catalyzing the first step of the steroidogenesis pathway, and play an important role in regulating the transport of cholesterol into the inner mitochondrial membrane [ 51 ]. The high star transcripts may increase the endogenous androgen levels, which compete with exogenous antiandrogen-like PrP for androgen receptors.…”

Section: Discussionmentioning

confidence: 99%

“…Fish reproduction is regulated by synergistic interactions between steroid hormones along the HPGL axis and by steroidogenesis in the gonad [ 51 ]. During steroidogenesis, the star gene is responsible for the rate-limiting transportation of cholesterol into the mitochondrial membrane, then, the cholesterol is stepwise converted to testosterone under the catalytic action of CYP11a1, 3β-HSD, CYP17a, and 17β-HSD, finally the testosterone is converted to 17β-estradiol by the aromatase (CYP19a) [ 86 ].…”

Section: Discussionmentioning

confidence: 99%

“…The reverse transcription of the total RNA was performed using the PrimeScript reagent Kit with gDNA Eraser (TaKaRa, Japan). A total of 19 target genes related to the hypothalamic–pituitary–gonadal–liver axis were selected to detect the mRNA expression in different samples, including the estrogen receptor genes ( erα and erβ ), androgen receptor genes ( arα and arβ ) [ 50 ], vitellogenin genes ( vtgB and vtgC ), cytochrome P450 genes ( cyp19a , cyp19a1a , cyp19a1b , cyp11a1 ), steroid 17-alpha-hydroxylase/17,20 lyase ( cyp17 ), gonadotropin releasing hormone ( gnrh ), gonadotropin releasing hormone receptor ( gnrhr ), hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase cluster ( hsd3b ), hydroxysteroid 17-beta dehydrogenase 3 ( hsd17b3 ), 20β-hydroxysteroid dehydrogenase type ( hsd20b ), sonic hedgehog ( shh ), patched 1 ( ptc1 ) as well as the steroidogenic acute regulatory protein ( star ) [ 51 , 52 ]. The glyceraldehyde-3-phosphate dehydrogenase gene ( gapdh ) was served as the endogenous reference gene [ 53 ].…”

Section: Methodsmentioning

confidence: 99%

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Endocrine Disruption of Propylparaben in the Male Mosquitofish (Gambusia affinis): Tissue Injuries and Abnormal Gene Expressions of Hypothalamic-Pituitary-Gonadal-Liver Axis

Song

et al. 2023

IJERPH

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Propylparaben (PrP) is a widely used preservative that is constantly detected in aquatic environments and poses a potential threat to aquatic ecosystems. In the present work, adult male mosquitofish were acutely (4d) and chronically (32d) exposed to environmentally and humanly realistic concentrations of PrP (0, 0.15, 6.00 and 240 μg/L), aimed to investigate the toxic effects, endocrine disruption and possible mechanisms of PrP. Histological analysis showed time- and dose-dependent manners in the morphological injuries of brain, liver and testes. Histopathological alterations in the liver were found in 4d and severe damage was identified in 32d, including hepatic sinus dilatation, cytoplasmic vacuolation, cytolysis and nuclear aggregation. Tissue impairments in the brain and testes were detected in 32d; cell cavitation, cytomorphosis and blurred cell boundaries appeared in the brain, while the testes lesions contained spermatogenic cell lesion, decreased mature seminal vesicle, sperm cells gathering, seminiferous tubules disorder and dilated intercellular space. Furthermore, delayed spermatogenesis had occurred. The transcriptional changes of 19 genes along the hypothalamus–pituitary–gonadal–liver (HPGL) axis were investigated across the three organs. The disrupted expression of genes such as Ers, Ars, Vtgs, cyp19a, star, hsd3b, hsd17b3 and shh indicated the possible abnormal steroidogenesis, estrogenic or antiandrogen effects of PrP. Overall, the present results provided evidences for the toxigenicity and endocrine disruptive effects on the male mosquitofish of chronic PrP exposure, which highlights the need for more investigations of its potential health risks.

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“…There is also ongoing research on the effects of endocrine disruptors on the transcriptional control of morphogenetic processes in endocrine organs [ 62 , 63 ].…”

Section: Studies On the Effects Of Low Doses Of Ddt On The Adrenal Glandsmentioning

confidence: 99%

Dichlorodiphenyltrichloroethane and the Adrenal Gland: From Toxicity to Endocrine Disruption

Timokhina

Yaglov

Назимова

2021

Toxics

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Endocrine disruptors are exogenous compounds that pollute the environment and have effects similar to hormones when inside the body. One of the most widespread endocrine disruptors in the wild is the pesticide dichlorodiphenyltrichloroethane (DDT). Toxic doses of DDT are known to cause cell atrophy and degeneration in the adrenal zona fasciculata and zona reticularis. Daily exposure in a developing organism to supposedly non-toxic doses of DDT have been found to impair the morphogenesis of both the cortex and the medulla of the adrenal glands, as well as disturbing the secretion of hormones in cortical and chromaffin cells. Comparison of high and very low levels of DDT exposure revealed drastic differences in the morphological and functional changes in the adrenal cortex. Moreover, the three adrenocortical zones have different levels of sensitivity to the disruptive actions of DDT. The zona glomerulosa and zona reticularis demonstrate sensitivity to both high and very low levels of DDT in prenatal and postnatal periods. In contrast, the zona fasciculata is less damaged by low (supposedly non-toxic) exposure to DDT and its metabolites but is affected by toxic levels of exposure; thus, DDT exerts both toxic and disruptive effects on the adrenal glands, and sensitivity to these two types of action varies in adrenocortical zones. Disruptive low-dose exposure leads to more severe affection of the adrenal function.

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Endocrine disrupting effects of binary mixtures of 17β-estradiol and testosterone in adult female western mosquitofish (Gambusia affinis)

Cited by 16 publications

References 61 publications

Activation of GPER by E2 promotes proliferation, invasion and migration of breast cancer cells by regulating the miR‑124/CD151 pathway

Activation of GPER by E2 promotes proliferation, invasion and migration of breast cancer cells by regulating the miR‑124/CD151 pathway

Endocrine Disruption of Propylparaben in the Male Mosquitofish (Gambusia affinis): Tissue Injuries and Abnormal Gene Expressions of Hypothalamic-Pituitary-Gonadal-Liver Axis

Dichlorodiphenyltrichloroethane and the Adrenal Gland: From Toxicity to Endocrine Disruption

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