Impact of redox-mediators in the degradation of olsalazine by marine-derived fungus, Aspergillus aculeatus strain bpo2: Response surface methodology, laccase stability and kinetics

Physicochemical characterization and antioxidant activity of polysaccharides from Chlorella sp. by microwave-assisted enzymatic extraction

“…The interactive influence of enzyme dosage, microwave power and extraction time on CSCP yield is shown in Figures 2A–F . It was reported that the steeper the slope in response surface, the greater the relationship between independent variables and CSCP yield ( Bankole et al, 2021 ; Ebrahimi et al, 2021 ). Conversely, the slope of the three-dimensional effect map in response surface is gentler, which demonstrates that the relationship between response value and variables is smaller, and the interactive influence of the two factors on CSCP yield is limited.…”

Bioprospection of ligninolytic enzymes from marine origin filamentous fungi

“…Laccase has a limited effect on bioremediation due to its specificity forlignin phenolic units and thus some substrates cannot be directly oxidized by Lac (Theerachat et al 2019), in these cases the limitation can be overcome with the addition of mediators, as well as some other enzymes, such as: feruloyl esterase, lipases, aryl-alcohol oxidase, catechol 2, 3-dioxigenase, quinone reductases (Kumar & Chandra 2020). Thus, it is necessary to optimize the adaptable subtract condition to these fungi, as well as to test different redox mediators concentrations, such as 2,20-azino-bis and p-cumárico acid, 1-hydroxybenzotriazol (Bankole et al 2021) or the inclusion of inductors, such as copper sulfate (De Salas et al 2019, Ferraroni et al 2017, ethanol, methanol, isopropanol, acetone, tween 80, glycerin, glucose, triton X, sulfate iron, calcium chloride, sodium nitrate (Aftab et al 2018), in order to improve the target enzyme activity.…”

“…Ligninolytic enzymes (LEs) are made up of three main representatives: lignin peroxidase (LiP,EC 1.11.1.14), manganese peroxidase (MnP,EC 1.11.1.13) and laccase (Lac, EC 1.10.3.2), along with others accessory enzymes (Wong 2009) and have the function of catalyzing modifi cations or degrading lignin into less complex molecules (Asemoloye et al 2020). These enzymes have great versatility that covers a wide spectrum of use in bioremediation, and it is pointed out its complex recalcitrant compounds degradation and mineralization capacity, such as petroleum compounds and their derivatives (Asemoloye et al 2020), pesticides (Zeng et al 2017), drugs, such as endocrine disrupters (Eldridge et al 2017) and polycyclic non-steroidal anti-inflammatory drugs (Bankole et al 2021), synthetic fragrances (Vallecillos et al 2017), lignocellulosic residues (Kumar & Chandra 2020) textile effluent dyes (Rather et al 2018) and also allows biocatalytic plastic recycling (Wei & Zimmermann 2017), bleaching paper (Nathan et al 2018) and biosensors production (Sidwaba et al 2019) among others. Most residues cited above have in their composition compounds that can cause adverse effects to the natural environment and to humans, among them polycyclic aromatic hydrocarbons (PAHs), formed by two or more condensed aromatic rings and responsible for several biological effects, such as high toxicity, mutagenicity and carcinogenic potential (Kadri et al 2017).…”

Marine-derived fungi as biocatalysts

“…Efforts to completely eliminate the drug in an eco-friendly manner have proven to be quite arduous due to the recalcitrant nature of the azo bond in its structure. Bankole et al (2021a) reported the degradation of 89.43% of olsalazine in 7 days by the marine-derived fungus Aspergillus aculeatus MT492456, isolated from saline soil samples collected off the Atlantic Ocean coast of Lagos. Biodegradation was carried out in the presence of the redox mediators azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), p-coumaric acid and 1-hydroxybenzotriazole, which promoted electron transfer during osalazine oxidation.…”