High-performance thin-layer chromatography in combination with a yeast-based multi-effect bioassay to determine endocrine effects in environmental samples

Baetz, Nicolai; Rothe, L.; Wirzberger, Vanessa; Sures, Bernd; Schmidt, Torsten; Tuerk, Jochen

doi:10.1007/s00216-020-03095-5

Cited by 16 publications

(10 citation statements)

References 42 publications

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“…The IC 10 s substantiate that. In another study, the application of yeast cells on the HPTLC plate for the detection of endocrine effects was compared [ 40 ]. An overall similar sensitivity was shown between spray and immersion approaches.…”

Section: Resultsmentioning

confidence: 99%

“…S4 ). In a previous study, investigating the application of a yeast suspension, the spray approach was slightly more precise than the immersion method [ 40 ]. Differences were the use of an airbrush to apply the yeast suspension onto the HPTLC plate until it was evenly moist.…”

Section: Resultsmentioning

confidence: 99%

“…Mendoza et al [ 20 ] and Stütz et al [ 23 ] used, as in this study, indoxyl acetate as an esterase substrate but each of them employed a different method to apply the esterase solution on thin-layer plates: immersion or spraying. In a previous work, an immersion and a spray method for application of yeast suspension on HPTLC plates were compared whereas a similar sensitivity and a better precision of the spraying approach were observed [ 40 ]. Schoenborn et al [ 41 ] sprayed yeast cells onto HPTLC plates and observed an unprecedented sensitivity of the planar yeast estrogen screen compared to immersion.…”

Section: Introductionmentioning

confidence: 99%

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High-performance thin-layer chromatography in combination with an acetylcholinesterase-inhibition bioassay with pre-oxidation of organothiophosphates to determine neurotoxic effects in storm, waste, and surface water

2022

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Pesticides such as organothiophosphates (OTPs) are neurotoxically active and enter the aquatic environment. Bioassays, using acetylcholinesterase (AChE), a suitable substrate and reactant, can be applied for the photometric detection of AChE-inhibiton (AChE-I) effects. The oxidized forms of OTPs, so-called oxons, have higher inhibition potentials for AChE. Therefore, a higher sensitivity is achieved for application of oxidized samples to the AChE assay. In this study, the oxidation of malathion, parathion, and chlorpyrifos by n-bromosuccinimide (NBS) was investigated in an approach combining high-performance thin-layer chromatography (HPTLC) with an AChE-I assay. Two AChE application approaches, immersion and spraying, were compared regarding sensitivity, precision, and general feasibility of the OTP effect detection. The oxidation by NBS led to an activation of the OTPs and a strong increase in sensitivity similar to the oxons tested. The sensitivity and precision of the two application techniques were similar, although the spray method was slightly more sensitive to the oxidized OTPs. The 10% inhibition concentrations (IC10) for the spray approach were 0.26, 0.75, and 0.35 ng/spot for activated malathion, parathion, and chlorpyrifos, respectively. AChE-I effect recoveries in samples from a stormwater retention basin and receiving stream were between 69 and 92% for malathion, parathion, and chlorpyrifos. The overall workflow, including sample enrichment by solid-phase extraction, HPTLC, oxidation of OTPs, and AChE-I assay, was demonstrated to be suitable for the detection of AChE-I effects in native water samples. An effect of unknown origin was found in a sample from a stormwater retention basin.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High-performance thin-layer chromatography in combination with an acetylcholinesterase-inhibition bioassay with pre-oxidation of organothiophosphates to determine neurotoxic effects in storm, waste, and surface water

2022

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“…Nach 45‐minütiger Inkubation bei Raumtemperatur wird die Detektion durchgeführt. Diese erfolgt über eine Messung der Fluoreszenz, wobei die Anregung bei einer Wellenlänge von 505 nm und die Detektion bei einer Wellenlänge von 670 nm mit einem DC‐Platten‐Scanner durchgeführt wird 6.…”

Section: Materialien Und Methodenunclassified

“…Zur Bestimmung endokriner Effekte wurden bspw. auf der Hefe Arxula adeninivorans 6 und auf der Hefe Saccharomyces cerevisiae basierende Assays (A‐YES) 5 entwickelt. Zur Bestimmung neurotoxischer Effekte wurde ein auf der Hemmung des Enzyms Acetylcholinesterase (AChE) basierender Assay 7, 8 entwickelt.…”

Section: Introductionunclassified

Vorteile von Open‐Source‐Ansätzen bei der Etablierung einer automatisierten wirkungsbezogenen Analytik

Jochums

Kochale

Teutenberg

et al. 2021

Chemie Ingenieur Technik

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Die Bestimmung der Wirkung von toxischen Verbindungen in Abwässern erfolgt mittels dünnschichtchromatographischer Verfahren. Für eine Automation der wirkungsbezogenen Analytik wurde eine bereits bestehende technische Lösung als Open‐Source‐Hardware‐Ansatz in Kombination mit der additiven Fertigung adaptiert. Das System ist flexibel auf spezifische Fragestellungen der automatisierten Bestimmung von umweltrelevanten Verbindungen anwendbar und leistet einen wertvollen Beitrag zur Digitalisierung und Automation von Hochleistungsdünnschichtchromatographie‐Systemen.

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Sample preparation for planar chromatography

Poole

2023

J of Separation Science

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High‐performance thin‐layer chromatography has favorable properties for high‐throughput separations with a high matrix tolerance. Sample preparation, however, is sometimes required to control specific matrix interferences and to enhance the detectability of target compounds. Trends in contemporary applications have shifted from absorbance and fluorescence detection to methods employing bioassays and mass spectrometry. Traditional methods (shake‐flask, heat at reflux, Soxhlet, and hydrodistillation) are being challenged by automated instrumental approaches (ultrasound‐assisted and microwave‐assisted solvent extraction, pressurized liquid extraction, and supercritical fluid extraction) and the quick, easy cheap, efficient, rugged, and safe extraction method for faster and streamlined sample processing. Liquid‐liquid extraction remains the most widely used approach for sample clean‐up with increasing competition from solid‐phase extraction. On‐layer sample, clean‐up by planar solid‐phase extraction is increasingly used for complex samples and in combination with heart‐cut multimodal systems. The automated spray‐on sample applicator, the elution head interface, biological detection of target and non‐target compounds, and straightforward mass spectrometric detection are highlighted as the main factors directing current interest toward faster and simpler sample workflows, analysis of more complex samples, and the determination of minor contaminants requiring high concentration factors.

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High-performance thin-layer chromatography in combination with a yeast-based multi-effect bioassay to determine endocrine effects in environmental samples

Cited by 16 publications

References 42 publications

High-performance thin-layer chromatography in combination with an acetylcholinesterase-inhibition bioassay with pre-oxidation of organothiophosphates to determine neurotoxic effects in storm, waste, and surface water

High-performance thin-layer chromatography in combination with an acetylcholinesterase-inhibition bioassay with pre-oxidation of organothiophosphates to determine neurotoxic effects in storm, waste, and surface water

Vorteile von Open‐Source‐Ansätzen bei der Etablierung einer automatisierten wirkungsbezogenen Analytik

Sample preparation for planar chromatography

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